Phosphoric acid

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Spain, Belgium, France, Germany, China, Australia, Italy

Phosphoric acid is a clear, colorless liquid that is commonly used in various laboratory applications. It serves as a chemical reagent and has a wide range of industrial applications. The core function of phosphoric acid is to act as a source of phosphorus, which is an essential element in many chemical processes and reactions.

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Lab products found in correlation

113 protocols using phosphoric acid

Topical Delivery of Botanical Compounds

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HSYA (purity>90%) was provided by Chengdu University (Chengdu, China). Icariin (batch number 489-32-7, purity>98%) was purchased from Shyuanye Biotechnology Co., Ltd (Shanghai, China). Cinnamaldehyde (batch number FY1265VX53J, purity>98%) and 3, 4-dihydroxybenzoic acid (batch number FY17830102, purity>98%) were obtained from Nantong Feiyu Biotechnology Co., Ltd (Nantong, China). Phospholipon 90G, Lipoid 75 andNatipide II were gifts from Lipoid GmbH (Newark, NJ, USA). Soybean lecithin was purchased from TCI America (Boston, MA, USA). Oleic acid (OA), isopropyl myristate (IPM), Tween 20, and Span 80 were purchased from Sigma (Saint Louis, MO, USA). Labrosol ALF, Labrafac 1349, Transcutol P were gifts from GATTEFOSSE (Paramus, NJ, USA). Phosphate buffer saline (PBS), Brij58, ethanol, propylene glycol, acetonitrile, and phosphoric acid were purchased from ACROS Organics (Newark, NJ, USA). All other ingredients and reagents were of analytical grade. Cadaver skin from the posterior torso (female, aged 56) was supplied by the NewYork Firefighters Skin Bank.

Lin H.M., Lin L.F., Sun M.Y., Liu J, & Wu Q. (2020). Topical Delivery of Four Neuroprotective Ingredients by Ethosome-Gel: Synergistic Combination for Treatment of Oxaliplatin-Induced Peripheral Neuropathy. International Journal of Nanomedicine, 15, 3251-3266.

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Preparation of Aqueous Buffers for HPLC

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HPLC grade methanol was purchased from Fisher Scientific (Pittsburgh, PA). Trifluoroacetic acid spectrophometric grade 99% was purchased from Aldrich Company (Milwaukee, WI). All water used in this study was filtered through a double deionized purification system (Milli Q Plus Water System) from Millipore Company (Bedford, MA).
pH 2.0 phosphate buffer was prepared using sodium phosphate dibasic heptahydrate from Acros Organics, potassium dihydrogen phosphate from Sigma-Aldrich, and phosphoric acid from Acros Organics. pH 4.5 acetate buffer was prepared using acetic acid purchased from Sigma-Aldrich and sodium acetate purchased from Sigma Chemical Company. pH 6.8 phosphate buffer was prepared using monobasic potassium phosphate purchased from Sigma- Aldrich and sodium hydroxide pellets (NaOH) from J.T. Baker (Philipsburg, NJ).

Cavanagh K.L., Maheshwari C, & Rodríguez-Hornedo N. (2017). Understanding the differences between cocrystal and salt aqueous solubilities. Journal of pharmaceutical sciences, 107(1), 113-120.

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Polymer Electrolyte Membrane Fabrication

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PBI (purity > 99.95%, MW 51000, with the molecular formula: (C₂₀H₁₂N₄)_n) was purchased from Danish Power Systems (Danish Power Systems, Kvistgaard, Denmark). LiCl, N,N-dimethylacetamide (DMAc) 99.8%, 1-butyl-3-methylimidazolium chloride (BMIM–Cl), 1-butyl-3-methylimidazolium bromide (BMIM–Br), 1-butyl-3-methylimidazolium iodide (BMIM–I), 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM–BF₄), 1-butyl-3-methylimidazolium hexafluorophosphate (BMIM–PF₆), 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (BMIM–NTf₂), and 1-butyl-3-methylimidazolium thiocyanate (BMIM–NCS) were purchased from Sigma–Aldrich (Sigma–Aldrich Química SL, Madrid, Spain). N,N–Dimethylacetamide (DMAc, 99.5% extra pure) and phosphoric acid (extra pure, 85% solution in water) were purchased from Acros Organics (Fisher Scientific SL, Madrid, Spain).

Escorihuela J., García-Bernabé A., Montero Á., Sahuquillo Ó., Giménez E, & Compañ V. (2019). Ionic Liquid Composite Polybenzimidazol Membranes for High Temperature PEMFC Applications. Polymers, 11(4), 732.

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Analytical Method Development for Ketoconazole

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Ketoconazole (lot # BS1203355108, 98% purity) was purchased from Bosche Scientific (New Brunswick, NJ) and used as received. Adipic acid (lot # 06807BE, 99% purity), succinic acid (lot # 037K0021, 99% purity), fumaric acid (lot # 09426EE, 99+% purity), acetic acid (lot # 074K3658, 99%), sodium acetate anhydrous (lot # 100K0272), dipotassium hydrogen phosphate (lot # 103H0287, ACS reagent), and sodium chloride (lot # 094K0183, ACS reagent) were purchased from Sigma-Aldrich (St. Louis, MO) and used as received.
HPLC grade methanol, HPLC grade 2-propanol, sodium phosphate monobasic (lot # 017316), and hydrochloric acid (lot # 2AJK15038, ACS grade) were purchased from Fisher Scientific (Fair Lawn, NJ). Acetone (ACS reagent 99.5%) and phosphoric acid (lot # B0506524, 85+%) were purchased from Acros Organics (NJ) and used as received. Trifluoroacetic acid (spectrophometric grade, 99%) was purchased from Aldrich Company (Milwaukee, WI). NaOH (pellets) was purchased from J.T. Baker (Philipsburg, NJ). Water used in this study was filtered through a double deionized purification system (Milli Q Plus Water System) from Millipore Co. (Bedford, MA).

Chen Y.M, & Rodríguez-Hornedo N. (2018). Cocrystals Mitigate Negative Effects of High pH on Solubility and Dissolution of a Basic Drug. Crystal growth & design, 18(3), 1358-1366.

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Characterization of Indomethacin Polymorphs

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Indomethacin γ-form (IND) and SAC were purchased from Sigma Chemical Company (St. Louis, MO) and used as received. IND polymorph γ was confirmed by differential scanning calorimetry (DSC) and powder X-ray diffraction (PXRD). Polyoxyethylene (20) oleyl ether (Brij99®), sodium lauryl sulfate (SLS) and polyoxyethylene (40) stearate (Myrj52®) were also purchased from Sigma Chemical Company (St. Louis, MO). High performance liquid chromatography (HPLC) grade acetonitrile and monosodium phosphate were purchased from Fisher Scientific (Fair Lawn, NJ). Phosphoric acid was purchased from Acros Organics (NJ). Water used in this study was filtered through a double deionized purification system (Milli Q Plus Water System) from Millipore Co. (Bedford, MA).

Stefan C.J., Overton M.C, & Blumer K.J. (1998). Mechanisms Governing the Activation and Trafficking of Yeast G Protein-coupled Receptors. Molecular Biology of the Cell, 9(4), 885-899.

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Compound Procurement for Pharmacokinetic Studies

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Tariquidar and Ko143 were obtained from Sigma. MK571 sodium salt (purity ≥98.0%) was purchased from MedChem Express. Probenecid was obtained from Fisher Scientific. Levofloxacin (purity ≥98.0%) was purchased from CSNpharm. Dimethyl sulfoxide (DMSO) (purity ≥99.5%), polyethylene glycol 300 (PEG300), Tween‐80, phosphoric acid (85 wt%), and Chromatographic‐grade methanol were purchased from Acros Organics. All other chemicals were commercially available and of the highest grade available.

Cen Y., Shan Y., Zhao J., Xu X., Nie Z, & Zhang J. (2022). Multiple drug transporters contribute to the brain transfer of levofloxacin. CNS Neuroscience & Therapeutics, 29(1), 445-457.

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Moringa oleifera Seed Extraction Protocol

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Moringa oleifera seeds were obtained commercially. Sigma-Aldrich (Suomi) supplied sodium chloride (NaCl 99%) and sodium hydroxide (NaOH 98.9%). Phosphoric acid (85 wt %) and diclofenac sodium salt (98.5%) were purchased from Acros (Geel, Belgium).

Bagheri A., Abu-Danso E., Iqbal J, & Bhatnagar A. (2019). Modified biochar from Moringa seed powder for the removal of diclofenac from aqueous solution. Environmental Science and Pollution Research International, 27(7), 7318-7327.

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Phosphoric Acid Quantification Protocol

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Phosphoric acid (for analysis, 85% solution in water, density 1.680) was purchased from Acros Organics (Fisher Scientific France, Illkirch, France). Deionized water (18 M) was produced by a Milli-Q apparatus (Millipore, Bedford, MA, USA). Acetonitrile was purchased as LC-MS grade from VWR (Fontenay-sous-bois, France). Phosphoric acid samples were prepared at a concentration of 5 mM using a volume of 0.035% of the initial Phosphoric acid solution in water/Acetonitrile (50/50 v/v). This 5 mM solution of Phosphoric acid was enriched with 50 µM ammonia to perform the collision induced experiments (CID) on the ammonia adducts of multiply charged Phosphoric acid cluster anions. Ammonium hydroxide solution 29.3% NH3 was from Sigma Chemical (St. Louis, MO, USA). The mixture of hexakis-(fluooalkyloxy)-phosphazines (Tuning Mix, Agilent Technologies) was diluted to 1:4 with 95% Acetonitrile in water and was used either alone or in mixture with Phosphoric acid.

Calabrese V., Lavanant H., Rosu F., Gabelica V, & Afonso C. (2020). Collision Cross Sections of Phosphoric Acid Cluster Anions in Helium Measured by Drift Tube Ion Mobility Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 31(4).

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Antimicrobial Susceptibility Screening

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Calcium chloride, sodium carbonate, citric acid, and phosphoric acid were purchased from Acros Organics (Geel, Belgium). Ammonium solution and Muller Hinton media were procured from Merck (Darmstadt, Germany), while diammonium hydrogen phosphate was purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium bicarbonate was purchased from Fisher Scientific Company (Waltham, MA, USA). All the materials were of the analytical grade. The local source provided amoxicillin as a gift sample. In addition, we obtained clinical strains of MRSA, P. aeruginosa, and E. coli from a research-based institute, the National institute for biotechnology and genetic engineering (NIBGE) Faisalabad, Pakistan.

Alotaibi N.H., Munir M.U., Alruwaili N.K., Alharbi K.S., Ihsan A., Almurshedi A.S., Khan I.U., Bukhari S.N., Rehman M, & Ahmad N. (2022). Synthesis and Characterization of Antibiotic–Loaded Biodegradable Citrate Functionalized Mesoporous Hydroxyapatite Nanocarriers as an Alternative Treatment for Bone Infections. Pharmaceutics, 14(5), 975.

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Synthesis of Porous Coordination Polymers

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Zinc nitrate tetrahydrate (for an EMSURE^® analysis), 2-Methylimidazole (Hmim with 99% purity), cobalt nitrate hexahydrate (99.999% trace metals basis), and lithium chloride (LiCl) were purchased from Sigma-Aldrich (Sigma-Aldrich Química SL, Madrid, Spain). N,N-Dimethylacetamide (DMAc, 99.5% extra pure) and phosphoric acid (extra pure, 85% solution in water) were purchased from Acros Organics (Fisher Scientific SL, Madrid, Spain). PBI (purity > 99.95%, MW 65000, with the molecular formula: (C₂₀H₁₂N₄)_n) was purchased from Danish Power Systems (Danish Power Systems, Kvistgaard, Denmark).

Escorihuela J., Sahuquillo Ó., García-Bernabé A., Giménez E, & Compañ V. (2018). Phosphoric Acid Doped Polybenzimidazole (PBI)/Zeolitic Imidazolate Framework Composite Membranes with Significantly Enhanced Proton Conductivity under Low Humidity Conditions. Nanomaterials, 8(10), 775.

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