Sk mel 2

B16F10 (mouse melanoma cell line), SK-MEL-2 (human melanoma cell line), SK-MEL-28 (human melanoma cell line), UACC-257 (human melanoma cell line), and HEK293 (human embryonic kidney cell line) cells were cultured in RPMI (Welgene, Gyeongsan, South Korea) with 10% fetal bovine serum (FBS, RMBIO, Missoula, MT, USA) and 1% antibiotic-antimycotic (Gibco, Grand Island, NY, USA). All cells were maintained at 37 °C with 5% CO₂ in a humidified chamber. UACC-257 was provided by the Chungnam National University Hospital (Daejeon, South Korea). B16F10, HEK293, SK-MEL-2, and SK-MEL-28 cells were purchased from the Korean Cell Line Bank (KCLB, Seoul, South Korea).

The human melanoma MNT-1 cell line was kindly provided by Dr. Ai-Young Lee at Dongguk University, who originally received it as a gift from Dr. Vincent J. Hearing at National Institutes of Health, Bethesda, Maryland, USA [42 (link)]. The cells were cultured at 37°C in MEM (Gibco, Carlsbad, CA, USA) containing 20% FBS, 10% DMEM (Lonza, Basel, Switzerland), 20 mM HEPES and antibiotics. The WM266-4 human melanoma cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). The SK-MEL-1, SK-MEL-2, SK-MEL-3, and SK-MEL-5 human melanoma cell lines were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea). Those cells were maintained at 37°C in RPMI1640 medium (Gibco) containing 10% FBS and antibiotics. Normal human melanocytes (Cascade Biologics, Portland, OR, USA) were cultured in M254 medium (Cascade) containing human melanocyte growth supplement-2 (Cascade). Endo H and PNGase F were purchased from New England Biolabs (Hitchin, UK).