Resolvin d1 elisa kit

The release kinetics of RvD1 were studied in collagen membrane (CM) discs of 6 mm diameter divided into two equal groups, biotinylated and non-biotinylated, using a resolvin D1 ELISA kit (Cayman Chemical). Within each group, either ethanol, 100 ng RvD1, or 800 ng RvD1 were added to the disks as described above. After lyophilization, discs were immersed in 3 mL of DPBS at 37 °C. Half of the buffer was collected at the following time points: 2, 5, 10, 24, and 192 h. In the 800 ng group, we added two more time points: 72 and 96 h. Every time a sample was collected, the buffer was replenished with an equal amount of fresh DPBS at 37 °C. The concentration, and hence the content, of RvD1 in each sample was calculated and a cumulative release curve was constructed for each group using the mean and se of four replicates.

RvD1, FPR2 receptor, TNF-α, and VEGF levels were measured in photoreceptors by using: Resolvin D1 ELISA Kit (Cayman Chemical, Cat# 500380), Mouse Formyl Peptide Receptor 2 (FPR2) ELISA Kit (MyBiosource, Cat# MBS764510), Mouse Interleukin 18 (IL-18) ELISA Kit (Abcam, Cat# ab216165), Mouse VEGF Quantikine ELISA Kit (RandD System, Cat# MMV00) in retinal photoreceptors and Human VEGF ELISA Kit (MyoBiosurce, Cat# MBS355343) in HUVEC cells.