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Ethovision xt 15

Manufactured by Noldus
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Sourced in Netherlands, United States, China

EthoVision XT 15 is a video tracking system designed for behavioral research. It automatically tracks and analyzes the movement and activity of animals in a variety of settings.

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Lab products found in correlation

Daniovision observation chamber, by Noldus (3 mentions) Daniovision system, by Noldus (2 mentions) Aca1300 60gc, by Basler (2 mentions) Nvoke2, by Inscopix (2 mentions) Mini io box system, by Noldus (2 mentions) Phenotyper cage, by Noldus (2 mentions) Y maze apparatus, by San Diego Instruments (2 mentions) Prism 7, by GraphPad (1 mentions) Stemi 508 microscope, by Zeiss (1 mentions) Stereo discovery v8 microscope, by Zeiss (1 mentions) Daniovision, by Noldus (1 mentions) Phytocannabinoids, by Merck Group (1 mentions) Hdr cx130, by Sony (1 mentions) Fear conditioning chamber, by Ugo Basile (1 mentions) Coolpix l340, by Nikon (1 mentions) Hdr cx270, by Sony (1 mentions) Hdr pj675, by Sony (1 mentions) Genicam, by Basler (1 mentions) Temperature control unit, by Noldus (1 mentions) Prism 9, by GraphPad (1 mentions)

114 protocols using ethovision xt 15

1

Behavioral Analysis of Stressed Fish

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Behavioral trials were conducted in the same order and not randomized. First, we examined behavior upon introduction to a new environment, as a proxy for stress (Chin et al., 2018 (link)). Second, we assayed aggression in response to a mirror. These first two trials were conducted on the same day in 19 L tanks separated by opaque dividers to prevent fish from seeing conspecifics during experiments. All trials were recorded continuously using one Wyze Cam v2 for each tank.
Next, the fish were acclimated to circular arenas for 70 h to test for wall-following behavior over a 1 h window. The fish were then returned to their 19 L experimental tanks, starved for three days, and tested for food consumption. After all behavioral measurements, fish were weighed, and photographs were taken to measure neuromast number, melanophore number, standard length, head depth, and eye diameter. All trials were conducted with tanks at room temperature (20–22 °C).
All video recordings of the trials were analyzed using EthoVision XT 15 (Noldus) behavioral tracking software to track subjects and calculate variables of interest. To ensure accurate tracking, each recording was reviewed manually, with erroneous data removed and subsequently interpolated using EthoVision XT 15 (Noldus). Fish videos with greater than 20% missing data were removed from their respective datasets prior to statistical analysis.
Swanson N.E., Gluesenkamp A.G., Donny A.E, & McGaugh S.E. (2023). Developmental environment contributes to rapid trait shifts among newly colonized subterranean habitats. Zoological Research, 44(4), 808-820.
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2

Open Field Exploration in Mice

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During the active phase, and in low light (<15 lux measured on arena floor), mice were placed in one corner of an open field arena (L43 × W43 × H35 cm) and given free access to explore for 10 min. Animal movement was detected with an automated 3-point software (Ethovision XT15, Noldus, US). For analysis, the arena was subdivided into sixteen equal zones (4 inner and 12 outer squares). Ethovision XT15 (Noldus, US) software calculated the time spent in each of the 16 zones. To measure time spent in center, the 4 inner zones were collapsed together.
Birnie M.T., Short A.K., de Carvalho G.B., Taniguchi L., Gunn B.G., Pham A.L., Itoga C.A., Xu X., Chen L.Y., Mahler S.V., Chen Y, & Baram T.Z. (2023). Stress-induced plasticity of a CRH/GABA projection disrupts reward behaviors in mice. Nature Communications, 14, 1088.
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3

Open Field Exploration in Mice

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Before the experiment, mice underwent a 30-minute habituation period in a behavioral laboratory. The open field box used had dimensions of 50 × 50 × 50 cm³, and a camera was positioned directly above it. During the experiment, mice were placed in the center of the open field box, and their movements were recorded for 5 minutes. Following the recording session, the mice were removed, and the inner walls and floor of the open field box were meticulously cleaned using 75% alcohol. The primary parameters assessed included the total distance traveled, mean speed, duration spent in the central square, and the number of times traversing the square. Ethovision XT 15 software (Noldus, Netherlands) was employed to analyze the footage for specific parameters, including total distance moved, mean velocity, and time spent in the center.
Chen S., Li M., Tong C., Wang Y., He J., Shao Q., Liu Y., Wu Y, & Song Y. (2024). Regulation of miRNA expression in the prefrontal cortex by fecal microbiota transplantation in anxiety-like mice. Frontiers in Psychiatry, 15, 1323801.
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4

Optogenetic Manipulation of Mouse Behaviors

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Optogenetic manipulation was performed using nVoke 2.0 (Inscopix). For optogenetic activation, 20 Hz, 60-pulse trains (5 ms each) of LED light (620 ± 30 nm, 20 mW/mm2) were initiated every 30 s during the light on epoch 48 (link). For optogenetic inhibition, constant LED light (620 ± 30 nm, 5 mW/mm2) was delivered during the light on epoch 49 (link). The EthoVision XT 15 software and a mini-IO box system (Noldus) were used to record live tracking of mice, and to trigger the laser on during specific behaviors.
Kin K., Francis-Oliveira J., Kano S.I, & Niwa M. (2023). Adolescent stress impairs postpartum social behavior via anterior insula-prelimbic pathway. bioRxiv.
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5

Larval Behavior Analysis Protocol

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At 7 days postfertilization (dpf), the larvae without morphological abnormalities were selected and transferred into 24-well plates with one larva in 2 mL solution per well. At 8 dpf, all larvae were removed from incubators and acclimated at room temperature for 2 h. Then, the movement behavior of individual larvae was continuously recorded for 10 min in dark conditions using a DanioVision system (Noldus, Wageningen, The Netherlands) after acclimation in an observation chamber for 10 min. The total distance traveled, average velocity, absolute sinuosity, absolute turn angle, and absolute angular velocity were analyzed using EthoVision XT15 software (Noldus, Wageningen, The Netherlands). Tests were carried out in triplicate (n = 24) for each concentration.
Gao F., Yuan Z., Zhang L., Peng Y., Qian K, & Zheng M. (2023). Toxic Effects of Copper Fungicides on the Development and Behavior of Zebrafish in Early-Life Stages. Nanomaterials, 13(19), 2629.
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6

Drosophila suzukii locomotion behavior

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Drosophila suzukii adult locomotion assays were performed in 9 cm diameter and 0.5 cm depth sterile Petri dishes that allowed free walking movement but restricted flight. Groups of eight 5–10-day-old conventional or axenic flies that had either been given open access to food or had been food-deprived for 15 h (provided with water) were placed into Petri dishes. Locomotion behaviors of flies were filmed in real-time using GigE cameras acA1300-60gc (Basler AG, Germany) for seven consecutive trials of 1 h duration from 12PM to 7PM on a laboratory bench under constant light condition and 23°C ambient temperature throughout the experiments. Each Petri dish contained eight flies that were tracked individually. Each fly in the assay was considered a replicate. For each experiment, four cameras were set up for four different groups of flies (female/male; conventional/axenic; and fed/starved), and each group was repeated once. Video footage was processed and analyzed in EthoVision XT 15 software (Noldus, Netherlands). Slightly modified from a previous study (Schretter et al., 2018 (link)), we set 0.3 mm/s and 0.1 mm/s as the threshold walking speeds for characterizing the start and stop of movement of the flies, respectively. The LOWESS (Locally Weighted Scatterplot Smoothing) method was applied to reduce the tracking noise and the small movements of the fly (“body wobble”).
Shu R., Hahn D.A., Jurkevitch E., Liburd O.E., Yuval B, & Wong A.C. (2021). Sex-Dependent Effects of the Microbiome on Foraging and Locomotion in Drosophila suzukii. Frontiers in Microbiology, 12, 656406.
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7

Footshock Sensitivity in Rats

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Rats were placed in a StartFear chamber (Panlab, Barcelona, Spain) and footshocks were delivered at 0.2, 0.25 and 0.3 mA for experiment 3 (to test whether there was any decrease in footshock sensitivity) and at 0.1, 0.15, 0.2 and 0.25 mA for experiment 5 (to test whether there was any increase in footshock sensitivity). For each intensity, triplicates were made with 20 s intervals, and 1 min was left before increasing the intensity. The footshock threshold was defined as a twitching, a freeze, or a jump off the grid. Footshock responses were recorded with a video-tracking system (EthoVision XT 15 software, Noldus Information Technology) and scored by two observers blind to the experimental conditions.
Goutaudier R., Joly F., Mallet D., Bartolomucci M., Guicherd D., Carcenac C., Vossier F., Dufourd T., Boulet S., Deransart C., Chovelon B, & Carnicella S. (2022). Hypodopaminergic state of the nigrostriatal pathway drives compulsive alcohol use. Molecular Psychiatry, 28(1), 463-474.
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8

Preference Assay for Fly Foraging

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Preference assays were performed in 9 cm diameter and 0.5 cm depth sterile Petri dishes that allowed free locomotion but restricted flight. Groups of eight 5–10 days old female flies that had been food-deprived for 15 h (provided with water) were placed into the dishes preloaded with sugar (sucrose)-yeast-casein diets at P:S=1:4 and P:S=1:22 (1cm diameter patches) on opposite sides of the dish (Figure 3A). Both diets had the same total sugar+yeast+casein concentration (90g/L). Relative protein content was manipulated by varying the quantity of casein hydrolysate (Thermo Scientific™), while the yeast concentration was kept constant (8.5g/L, MP Biomedicals) to minimize differences in yeast volatiles and other nutrient components between the diets. (Lihoreau et al., 2016 (link)). Fly foraging behavior was recorded in real-time using GigE cameras acA1300-60gc (Basler AG, Germany) for 6 hours under constant light condition and 23°C ambient temperature. Video footage was processed and analyzed by the EthoVision XT 15 software (Noldus, Netherlands). The LOWESS (Locally Weighted Scatterplot Smoothing) method was applied to reduce the tracking noise and the small movements of the fly (“body wobble”).
Shu R., Uy L, & Wong A.C. (2021). Nutritional phenotype underlines the performance trade-offs of Drosophila suzukii on different fruit diets. Current Research in Insect Science, 2, 100026.
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9

Water Maze Assessment of Cognitive Function

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Cognitive function was determined using the water maze test as previously described with some modifications (Ma et al., 2013 (link)). Mice were first subjected to the positioning and navigation test for five consecutive days and four trials per day after BCAS surgery. The swimming speed and escape latency (water maze-learning) were recorded. The probe test (water maze-memory) was conducted in the absence of the platform with a cut-off time of 120 s on the sixth day. The first latency to the platform, time spent in quadrant, frequency of crossing the platform area and track plots were collected. Data were analyzed using the Ethovision XT 15 software (Noldus Inc.).
Zhang Z., Guo Z., Tu Z., Yang H., Li C., Hu M., Zhang Y., Jin P, & Hou S. (2023). Cortex-specific transcriptome profiling reveals upregulation of interferon-regulated genes after deeper cerebral hypoperfusion in mice. Frontiers in Physiology, 14, 1056354.
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10

Investigating Locomotor Activity Modulation

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To investigate the role of A14 neurons and their neurotransmitter systems in defining locomotor activity, we used different viruses (Fig. 5a, Supplementary Fig. 7e) including negative controls (Supplementary Fig. 7c, d). Seventeen days after the last surgery, animals were single housed in PhenoTyper cages (Noldus) with one day of habituation and 6 days of continuous activity measurements. Food and water were available ad libitum. DREADDs were stimulated by diluting CNO in the drinking water (5 mg/kg, calculated upon average water intake being 5 ml per day) with 2–3 experimental repeats for each animal. Home-cage activity was analyzed from CT 00:00–06:00 (“day-time”) and CT 12:00–18:00 (“nighttime”). The following parameters were defined: distance moved (m), total activity (%), walking duration (%), and wheel running (number of wheel rotations). At the end of the experiments, the animals were sacrificed, with their brains used for post-hoc verification of the injection sites. Data were analyzed using EthoVision XT15 software (Noldus) and GraphPad Prism 7 (GraphPad).
Korchynska S., Rebernik P., Pende M., Boi L., Alpár A., Tasan R., Becker K., Balueva K., Saghafi S., Wulff P., Horvath T.L., Fisone G., Dodt H.U., Hökfelt T., Harkany T, & Romanov R.A. (2022). A hypothalamic dopamine locus for psychostimulant-induced hyperlocomotion in mice. Nature Communications, 13, 5944.
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