Cd4 cre mice

C57BL/6 mice were purchased from Charles River. We previously generated Ikzf2^fl/fl mice (8 (link)). These mice were crossed to CD4Cre mice, obtained by The National Institute of Allergy and Infectious Diseases (NIAID) and maintained under contract by Taconic Biosciences (Germantown, NY), to generate Ikzf2^fl/fl × CD4Cre mice. Ikzf2^fl/fl × CD4Cre mice were then crossed to OT-II × Foxp3^GFP mice to generate OT-II-Foxp3^GFP × Ikzf2^fl/fl -CD4Cre mice. OT-II TCR transgenic mice and Foxp3^GFP reporter mice were also obtained by (NIAID) and maintained under contract by Taconic Biosciences (Germantown, NY). Congenic F1 SAP^−/− mice were kindly provided by Dr. Pam Schwartzberg (NIAID, NIH). LCMV GP_61-80-specific TCR transgenic SMARTA mice (15 (link)) were originally obtained from the La Jolla Institute of Allergy and Immunology and then crossed at NIH to RAG1^−/− mice that were maintained for NIAID under contract by Taconic Biosciences (Germantown, NY). SMARTA × RAG^−/− mice were further crossed in our lab to B6.SJL (CD45.1) mice. B6.SJL, RAG1^−/−, B6.SJL OT-II RAG1^−/−, and C57BL/6J × B6.SJL F1 mice were obtained by NIAID and were maintained by Taconic Biosciences (Germantown, NY). In all experiments, both male and female mice were used. All animal protocols used in this study were approved by the NIAID Animal Care and Use Committee.

All the mice in this study were maintained in Specific Pathogen Free (SPF) facilities at Northwestern University or University of Florida. Mice were littermates and were 6–8 weeks old unless otherwise indicated in the text. Ikzf1^ΔF4/ΔF4, Ikzf1^f/f and Ahr^−/− mice were described previously and are backcrossed to C57BL/6 background (Fernandez-Salguero et al., 1995 (link); Heizmann et al., 2013 (link); Schjerven et al., 2013 (link)). Cd4-cre mice were purchased from Taconic Farms. Rorc-cre mice and Rorc^gfp/gfp mice were generated previously (Eberl and Littman, 2004 (link); Sun et al., 2000 (link)). All studies with mice were approved by the Animal Care and Use Committee of Northwestern University and University of Florida.

S1pr1^loxp/loxp mice were kindly provided by Dr. Richard Proia (National Institutes of Health) and crossed with CD4-Cre mice (Taconic) to generate S1pr1 deletion in T cells (S1pr1^−/−). Stat3^loxp/loxp mice were kindly provided by Drs. Shizuo Akira and Kiyoshi Takeda (Osaka University, Japan) and crossed with CD4-Cre mice to generate Stat3 deletion in T cells (Stat3^−/−). Transgenic mice expressing human S1pr1 under the control of the human CD2 promoter (S1pr1-Tg) were kindly provided by Dr. Markus Gräler (Hannover Medical School, Germany). S1pr1-Tg mice were crossed with the CD4-Cre/Stat3^loxp/loxp mice to generate mice with S1pr1-Tg/Stat3^−/− T cells. Foxp3-GFP knock-in mice were obtained from Dr. Defu Zeng (City of Hope) and subsequently crossed with S1pr1-Tg mice. C57BL/6 mice were purchased from the National Cancer Institute. Rag1^−/− and Rag2^−/− mice were obtained from the Jackson Laboratory. Mouse care and experimental procedures were performed in accordance with established institutional guidance and approved protocols from Institutional Animal Care and Use Committee at the Beckman Research Institute of City of Hope National Medical Center.