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Hsp90 antibody

Manufactured by Enzo Life Sciences
Sourced in Germany, United States

The HSP90 antibody is a laboratory reagent used to detect and quantify the presence of the Heat Shock Protein 90 (HSP90) in biological samples. HSP90 is a highly conserved molecular chaperone protein that plays a crucial role in the folding, stability, and function of various client proteins. The HSP90 antibody can be used in various analytical techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of HSP90 in cells and tissues.

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2 protocols using hsp90 antibody

1

Immunoblot Analysis of Key Proteins

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Western blot was done as previously described[20 (link)]. Antibodies specific for the following targets were from Santa Cruz (Heidelberg, Germany): BAX, histone deacetylase 2 (HDAC2), HDAC1, and p53. Anti-survivin was obtained from Novus Biologicals (Cambridge, United Kingdom). HSP90 antibody was provided by Enzo Life Sciences (Lörrach, Germany).
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2

Multi-Omics Evaluation of Anticancer Compounds

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Vitexin, acridine orange (AO), Tricaine, 5-fluorouracil (5-FU), cisplatin (CIS), docetaxel (DOC), vincristine (VIN), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Hoechst 33342, 3-methyladenine (3-MA), dimethylsulfoxide (DMSO), dichloro-dihydrofluorescein diacetate (DCFH-DA) and other chemicals, unless stated otherwise, were purchased from Sigma-Aldrich (St. Louis, MO, USA). β-actin, MDR-1, HSF-1, lamin B, HSP70, HSP27, BID, BAX, cytochrome C, caspase-3, caspase-9, and Bcl-2 antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). HSP90 antibody was purchased from Enzo Life Science (Farmingdale, NY, USA). BECN-1 and ATG5 were purchased from Cell Signaling Technology (Beverly, MA, USA). LC3 was obtained from Abcam (Cambridge, MA, USA). Horseradish peroxidase (HRP)-conjugated secondary antibodies for western blot analysis were purchased from Santa Cruz Biotechnology.
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