HL-60 cells were obtained from NCI 60-Panel. Jurkat and MV4-11 cells were obtained from ATCC. OCI-AML5, RS4;11, SEM, ML-2, MOLM-13, MOLM14, NOMO-1, OCI-AML2, KOPN-8, EOL-1, and OCI-AML3 cells were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Braunschweig, Germany). All used cells were cultured in the appropriate media and conditions.
Oci aml2
Manufactured by Leibniz Institute DSMZ
Sourced in Germany, United States
The OCI-AML2 is a compact and automated microbial identification system developed by the Leibniz Institute DSMZ. The device utilizes matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology to rapidly and accurately identify a wide range of microorganisms from various sample types.
Automatically generated - may contain errors
Lab products found in correlation
Oci aml3, by Leibniz Institute DSMZ (21 mentions)
Mv4 11, by Leibniz Institute DSMZ (15 mentions)
Molm 13, by Leibniz Institute DSMZ (12 mentions)
Thp 1, by American Type Culture Collection (10 mentions)
Mycoalert mycoplasma detection kit, by Lonza (9 mentions)
Hl 60, by Leibniz Institute DSMZ (7 mentions)
Hek293t, by American Type Culture Collection (7 mentions)
Thp 1, by Leibniz Institute DSMZ (6 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (6 mentions)
Nomo 1, by Leibniz Institute DSMZ (5 mentions)
Rpmi 1640, by Thermo Fisher Scientific (5 mentions)
Jurkat, by American Type Culture Collection (5 mentions)
Kasumi 1, by Leibniz Institute DSMZ (5 mentions)
Thp 1 cell, by American Type Culture Collection (5 mentions)
Set 2, by Leibniz Institute DSMZ (4 mentions)
Hl 60, by American Type Culture Collection (4 mentions)
Raw 264.7 cells, by American Type Culture Collection (4 mentions)
Hek293t cell, by American Type Culture Collection (4 mentions)
Penicillin streptomycin solution, by Beyotime (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
32 protocols using oci aml2
1
Cell Line Procurement and Culture
2
Detailed Cell Line Cultivation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All human cell lines were cultured in RPMI-1640 supplemented with 10% fetal bovine serum (FBS) (from here on termed R10) or in the recommended medium. The following cell lines were used: T2, THP-1, and HS-5 (from American Type Culture Collection, Manassas, VA, USA); Set-2, OCI-AML2, OCI-AML3, and U2940 (from Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany); PB2B and MAC-2A (a kind gift from Niels Ødum, University of Copenhagen, Denmark); K562 cells stably transduced with HLA-A2 or HLA-A3; K562-A2 and K562-A3 (a kind gift from Mariam Heemskerk, University Hospital Leiden, The Netherlands); Marimo (a kind gift from Steffen Koschmieder, Universitätsklinikum Aachen, Germany); and UKE-1 (a kind gift from W. Fiedler, University Hospital of Eppendorf, Germany). PB2B and MAC-2A were from cutaneous lymphoma; Marimo, OCI-AML2, OCI-AML-3, THP-1, Set-2, and UKE-1 from acute myeloid leukemia (AML); and U2960 from diffuse large B cell lymphoma. The murine cancer cell line EO771.LMB (a kind gift from Janine Erler, University of Copenhagen, Denmark, and originally described by Johnstone et al.28 (link)) was maintained in Dulbecco’s modified Eagle´s medium (DMEM) with 20% FBS, 20 mM HEPES, and penicillin and streptomycin (Pen/Strep) to a final concentration of 100 U/ml and 100 μg/ml, respectively. All cell lines were tested and confirmed negative for mycoplasma.
3
Cell Line Cultivation for Myeloid Leukemia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The OCI-AML3 and OCI-AML2, human myeloid leukemia cell lines, were bought from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, NI, Germany) and cultivated in RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA, #11875093) containing 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, #10099141C) and 1% penicillin-streptomycin solution (P-S) (Beyotime, Shanghai, China, #C0222). The THP-1, NB4, and KG-1a, human myeloid leukemia cell lines, were acquired from the American Type Culture Collection (ATCC, Manassas, VA, USA) and grew in RPMI-1640 with 10% FBS and 1% P-S. The human embryonic kidney (HEK) 293T cells were obtained from the ATCC and cultivated in DMEM with 10% FBS (Thermo Fisher Scientific, #10091155) and 1% P-S. The aforementioned cells were incubated at 37 °C with 5% CO2.
4
Cell Lines Acquisition and Culturing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell lines were acquired from American Type Culture Collection (ATCC) or the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), as indicated: HEK293T cells (ATCC #CRL-3216), MOLM13 (DSMZ Cat#ACC554), MV4;11 (ATCC Cat#CRL-9591), OCI-AML2 (DSMZ Cat#ACC-99), THP-1 (ATCC Cat#TIB-202), OCI-AML3 (DSMZ Cat#ACC-582), U937 (ATCC Cat#CRL-1593.2), and HL60 (ATCC Cat#CCL-240). The IMS-M2 cell line (NPM1c) was a kind gift from Dr. Daniel Tenen, Harvard Medical School, with original source as previously described70 . Identification cell lines was independently confirmed by cytogenetics profiling. Routine mycoplasma testing was negative. Cells were cultured in RPMI 1640 (Gibco Cat #11875–093) supplemented with 10% foetal bovine serum, 100U/mL penicillin (Gibco), 100 μg/mL streptomycin (Gibco), and L-glutamine 2 mM at 37°C and 5% CO2.
5
Cell Lines Acquisition and Culturing
6
Generation and Characterization of FLT3-ITD Cell Lines
7
Cell Line Cultivation Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AML-derived THP-1 and KG-1a cells were purchased from RIKEN biological resource center (BRC, Japan). AML-derived Kasumi-1, HL60, SKNO-1, NOMO-1 cells and embryonic kidney derived HEK293T cells were from Japanese Collection of Research Bioresources (JCRB, Japan). AML-derived KO52, NB4, ME-1, ML-2, OCI-AML2, MV4-11, MOLM-13 and HEL cells were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Germany). HEK293T cells were maintained in Dulbecco's modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% Penicillin–Streptomycin (PS) in a humidified incubator with 5% CO2 and 95% air at 37 °C. The other cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium containing 10% FBS and 1% PS under 5% CO2 and 95% air at 37 °C.
8
AML Cell Lines and Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acute Myeloid Leukemia (AML) cell lines F-36P, HL-60, KASUMI-1, MOLM-13, MONO-MAC-6, MV-4-11, NB-4, NOMO-1, OCI-AML2, OCI-AML3, and THP-1 were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Germany). Cell lines were maintained at 37°C and 5% CO2 in appropriate media (Supplementary Table 1 ). 293T cells were cultured in DMEM (Gibco, Germany) along with 10% FBS (Biochrom, Germany). Cell lines were routinely verified for mycoplasma contamination using Venor®GeM Mycoplasma Detection Kit (Sigma-Aldrich, Germany). Cell lines were authenticated by Multiplexion, Germany. The inhibitors were purchased from Abcam (UK), Biozol (Germany), and Santa Cruz Biotechnology (US).
9
Detailed Cell Line Verification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
OCI-AML2, OCI-AML3, KG-1, MV4–11, THP-1 and Molm-13 were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen and American type Culture Collection repositories and cultured under their instructions. Chemo-resistant cell lines were generated as described previously22 (link). All cell lines were verified by short tandem repeat (STR) analysis and tested for mycoplasma contamination by ICBR sequencing core at University of Florida.
10
Generation and Characterization of Myeloid Leukemia Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human myeloid leukemia cell lines OCI-AML2 and OCI-AML3 were obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, NI, Germany). The OCI-AML3 cells carry NPM1 mutation type A (NPM1-mA). Stable NPM1-mA-GFP expressing OCI-AML2 cells (OCI-AML2 + NPM1-mA) were generated (Additional file 6 : Figure S1a-c) using lentiviral transduction and selected with 5–10 μg/mL blasticidin and fluorescence-activated cell sorter using a FACSCalibur-Sort instrument (Becton Dickinson Biosciences, Copenhagen, Denmark) equipped with a 15-mW argon ion laser (488 nm) for excitation. Human myeloid leukemia cell lines KG-1a, NB4, THP-1, HL-60, U937 and human embryonic kidney cell line HEK293T were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). These myeloid leukemia cell lines were cultured in RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific) and 1% penicillin/streptomycin solution (Beyotime, Shanghai, China). HEK293T cells were maintained in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin solution. All cell lines were incubated at 37 °C in the presence of 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!