Illustrator 2022

All figures were generated using a combination of ImageJ, MATLAB (both versions R2020b and R2022a), Inkscape (version 1.1.2), and Adobe Illustrator 2022 (version 26.1). ImageJ was used for figures showing histology. MATLAB produced numerical plots. Inkscape was used to compile and complete these figures, with some help from Adobe Illustrator. Videos were produced using ImageJ and Adobe Media Encoder 2022 (22.61).

All graphs were produced and statistical analysis were performed in GraphPad Prism Version 9.4.0 (GraphPad Software LLC). Figures were compiled using Adobe Illustrator 2022 Version 26.2.1 (Adobe).

All analyses were performed using SPSS version 25 (SPSS, Inc., Chicago, IL, USA). Figures were plotted using GraphPad Prism 5 (version 5.02; GraphPad Software, La Jolla, CA, USA) and Adobe Illustrator 2022 (version 26.5; Adobe Inc., San Jose, CA, USA). All variables were assessed for normality with Kolmogorov–Smirnov and Shapiro–Wilk tests. Comparisons between groups were performed by Chi-square for categorical variables, t-test for continuous variables with normal distribution, or Mann–Whitney U test for continuous variables with skewed distribution. The bivariate relationships were assessed using the Spearman or Pearson correlation coefficient. Repeated measurements were assessed by one-way repeated ANOVA followed by post hoc comparisons. Inter-group analysis on differences in repeated measurements between the IG and CG of IIM patients were assessed by two-way repeated (group × time) ANOVA. Data are presented as median (inter-quartile range) unless stated otherwise, and p < 0.05 was considered statistically significant.

Data were processed using Microsoft Excel 2019. One–way analysis of variance (ANOVA) was conducted to compare the effect of different treatments. The effects of the same tillage cultivations on different straw returning methods were identified using SPSS 23.0 software. Statistical significance was determined at P = 0.05 [27 ]. The relationship between characterization indexes was determined using Pearson’s correlation matrix in RStudio [28 ]. Diagrams and tables were drawn using AUTOCAD 2019, Origin 2019, and Adobe Illustrator 2022.

Diagrams shown in Figs. 1a, 2c, 2f, 4b, 5m, 6a, 7a, b, 10 and Supplementary Fig. 1a, b, and 7a were created using adobe illustrator 2022 (version 26.3.1, Adobe).

For statistical analysis of studies consisting of two groups, unpaired two-tailed t-tests were applied. When comparing more than two study groups, 1-way or 2-way analysis of variance (ANOVA) with multiple comparison post hoc tests as specified were used to assess statistical difference between samples. The number of individual biological replicates (n values) and exact P values are shown in the figures or schematics. The threshold of statistical significance (α) was set to 0.05. Source data and statistical analysis are shown in the S5 Data and S6 Data files, respectively. Statistical analyses were carried out in Prism version 10.0.3 (GraphPad), graphs were assembled in R, Prism 10.0.3, Adobe illustrator 2022, or PyMOL. Sequence alignments were performed with MAFFT version 7 and Jalview 2.11.2.7. Cartoons and study design overviews were created in Biorender and assembled in Adobe illustrator 2022.