The levels of FSH, luteinizing hormone (LH), testosterone and oestradiol were determined using the chemiluminescence method (Siemens, Germany). The levels of anti-Müllerian hormone (AMH) were determined by the immunoelectrochemiluminescence method (Roche, Austria). In order to determine AMH, due to the weakness of the method, we often had to use dilutions of the sample.
Chemiluminescence method
Manufactured by Siemens
Sourced in Germany
The Chemiluminescence method is a laboratory technique that measures the light produced by a chemical reaction. This method is used to detect and quantify various analytes, such as proteins, enzymes, and small molecules. The core function of this technique is to generate and measure the light emission that occurs during specific chemical reactions.
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Lab products found in correlation
4 protocols using chemiluminescence method
1
Hormone Levels Determination Protocol
2
Assessment of Urological Symptoms and Prostate Characteristics
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All participants underwent a urological examination, including measurement of serum prostate-specific antigen (PSA) level and prostate volume (PV) and completion of an International Prostate Symptom Score (IPSS) questionnaire. PSA level was assayed with commercially available kits using the chemiluminescence method (Siemens, Malvern, PA, USA). PV was determined using transrectal ultrasonography, which was performed using a 7.5-MHz rectal probe (Aloka, Tokyo, Japan); PV was then calculated using the ellipsoid formula {PV=π/6×[width (cm)×thickness (cm)×length (cm)]} [12 (link)]. The IPSS questionnaire is composed of three domains related to storage symptoms (frequency, urgency, and nocturia), four domains related to voiding symptoms (hesitancy, weak stream, intermittence, and incomplete emptying), and one domain related to QoL. Each IPSS domain uses a 6-point scale ranging from 0 (none) to 5 (5 or more). The total score for symptoms was defined as the sum of scores for seven domains: the total score ranges from 0 to 35, with a higher score indicating a higher severity of symptoms [3 (link)]. Uroflowmetry data (Medtronics, Minneapolis, MN, USA) were collected to calculate maximal flow rate (Qmax). Qmax <10 mL/sec reflects the presence of bladder outlet obstruction, such as that which occurs in BPH [3 (link)].
3
Circadian Rhythm Assessment in UAA and PBMAH
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Plasma levels of cortisol (midnight, 8 AM, 4 PM), ACTH (midnight, 8 AM, 4 PM), and 24 hours urine free cortisol (UFC) were determined using the chemiluminescence method (Siemens, Germany). The standard reference ranges of cortisol at 8 AM, 4 PM, and midnight are 198.7 to 797.5 nmol/L, 85.3 to 459.6 nmol/L, and 0 to165.7 nmol/L. The normal reference range of ACTH at 8 AM, 4 PM, and midnight is <10.12 pmol/L. The normal reference range of 24 hours UFC is 98.0 to 500.1 nmol/24 h.
Blood samples of 4 patients with UAA and 14 patients with PBMAH were taken every 2 hours during 24 hours for assessment of plasma ACTH and cortisol concentrations. This exploratory hormone level monitoring can help further compare the 24-hour ACTH and cortisol rhythms between the two groups.
Blood samples of 4 patients with UAA and 14 patients with PBMAH were taken every 2 hours during 24 hours for assessment of plasma ACTH and cortisol concentrations. This exploratory hormone level monitoring can help further compare the 24-hour ACTH and cortisol rhythms between the two groups.
4
Bone Metabolism in Childhood Diabetes
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Szymańska M., Michałus I., Kaszkowiak M., Wyka K., Chlebna-Sokół D., Fendler W., Jakubowska-Pietkiewicz E., Młynarski W., Szadkowska A., Zmysłowska A.
of clinical diabetes diagnosis and after an average of seven (6-8) months of follow-up. In the control group the bone turnover parameters were determined only once. Serum osteocalcin levels were evaluated by the electrochemiluminescence method (ECLIA) (Roche Diagnostics GmbH, Germany), whereas urine DPD levels were assessed using the chemiluminescence method (Siemens, UK). Serum OPG and s-RANKL levels were assessed by the ELISA method (respectively, BI-20403, BIOMEDICA, Austria and BI-20462, BIOMEDICA, Austria).
The body mineral density was evaluated by DXA using a Prodigy device (GE Lunar, US) in total body projection (TBLH) and lumbar projection (L1-L4). BMDs results were interpreted as the Z-score index referring to the mean and standard deviation for age and gender [15] (link).
Results of metabolic bone markers were also related to parameters of the clinical course of diabetes, including: pH at T1D onset, HbA 1c level at onset and at follow-up (%), BMI-Z-score at onset and at follow-up, and both fasting and stimulated C-peptide levels (ng/ml) at two time points.
of clinical diabetes diagnosis and after an average of seven (6-8) months of follow-up. In the control group the bone turnover parameters were determined only once. Serum osteocalcin levels were evaluated by the electrochemiluminescence method (ECLIA) (Roche Diagnostics GmbH, Germany), whereas urine DPD levels were assessed using the chemiluminescence method (Siemens, UK). Serum OPG and s-RANKL levels were assessed by the ELISA method (respectively, BI-20403, BIOMEDICA, Austria and BI-20462, BIOMEDICA, Austria).
The body mineral density was evaluated by DXA using a Prodigy device (GE Lunar, US) in total body projection (TBLH) and lumbar projection (L1-L4). BMDs results were interpreted as the Z-score index referring to the mean and standard deviation for age and gender [15] (link).
Results of metabolic bone markers were also related to parameters of the clinical course of diabetes, including: pH at T1D onset, HbA 1c level at onset and at follow-up (%), BMI-Z-score at onset and at follow-up, and both fasting and stimulated C-peptide levels (ng/ml) at two time points.
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