Poly(ethylene glycol) methyl ether (4-cyano-4-pentanoate dodecyl trithiocarbonate) (PEG-CTA), 2-diisopropylaminoethyl methacrylate (DIPAMA), and pyridyl disulfide ethyl methacrylate (PDSEMA) were purchased from Sigma (Saint Louis, MO, USA). Fetal bovine serum (FBS) was purchased from R&D Systems (Minneapolis, MN, USA). Anti-mouse CTLA-4 (clone 9H10) and PD-1 (clone 29F.1A12) antibodies were purchased from Bio X cell. All other chemicals were purchased from Sigma and used as received.
Pd 1 clone 29f 1a12
Manufactured by BioXCell
The PD-1 (clone 29F.1A12) is a monoclonal antibody that recognizes the programmed cell death-1 (PD-1) receptor. PD-1 is an immune checkpoint protein expressed on the surface of T cells, B cells, and natural killer cells. The PD-1 (clone 29F.1A12) antibody can be used to detect and study the expression of PD-1 on various cell types.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pd 1 clone 29f 1a12
1
PEG-CTA Polymer Synthesis
2
Intratumoral ZIKV and Checkpoint Blockade
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At day 7 after tumor implantation, mice with similar flux were randomized between groups. Using the same coordinates as for tumor implantation, mice were inoculated intratumorally with mouse-adapted ZIKV (105 FFU), Δ10 3′-UTR-ZIKV (106 FFU), or PBS, each in 10 μL. We injected CD8-depleting antibodies (clone 2.43, Bio X Cell) or isotype control IgG2b (clone LTF-2, Bio X Cell) intraperitoneally starting at day 14 after tumor implantation with an initial dose of 25 mg/kg and followed with booster doses of 12.5 mg/kg every 5 days until day 26. Representative mice were bled to confirm depletion. Checkpoint blockade antibodies against PD-1 (clone 29F.1A12, Bio X Cell), or corresponding IgG2a control (2A3, Bio X Cell), were injected intraperitoneally on days 8, 10, 12, and 14 with a dose of 10 mg/kg. Mice were monitored daily for signs of neurological impairment and were euthanized when moribund. Animal caretakers were blinded to treatments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!