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4 protocols using isopropanol ipa

1

Synthesis of Cerium-Nickel Nanocomposites

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There was no further purification of any of the chemicals used in the study since all of them were of analytical grade. Ce(NO3)3.6 H2O and Ni(NO3)2. 6 H2O were purchased from Fluka. Ethylenediaminetetraacetic acid disodium (EDTA-2Na) and ethanol were purchased from BDH Chemicals (England). 1, 4-benzoquinone (BQ), and Isopropanol (IPA) were purchased from Scharlau (Spain). Cetyltrimethylammonium Bromide (CTAB) and Ammonium hydroxide were purchased from Sigma-Aldrich and Merch, respectively.
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2

Photopolymer Resin and Metal Salt Synthesis

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Clear photopolymer standard resin (a mixture of proprietary acrylic monomers and oligomers and phenylbis (2,4,6-trimethyl benzoyl)-phosphine oxide as a photoinitiator) was purchased from XYZprinting, Inc (XYZprinting, New Taipei City, Taiwan). Silver nitrate (AgNO3) was purchased from VWR Chemicals, silver perchlorate (AgClO4) was purchased from Alfa Aesar, and isopropanol (IPA) was purchased from Scharlau. All products were used as received.
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3

Metabolite Extraction and Derivatization

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For the sample preparation, LC-MS-grade solvents and reagents were used. Methoxyamine, N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA), tridecanoic acid, and hexadecane were purchased from Merck (Darmstad, Germany); water, acetonitrile, and pyridine were obtained from VWR (Milano, Italy); isopropanol (IPA) was from Scharlab (Barcelona, Spain).
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4

Liquid Chromatography-Mass Spectrometry Materials

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Ultra-pure water was generated employing a Milli-Q Integral Water Purification System from Merck Millipore (Darmstadt, Germany). LC-MS grade solvents (CH 3 CN, isopropanol (IPA), and CH 3 OH) were acquired from Scharlau (Barcelona, Spain). Formic acid (≥95%), and ammonium acetate (≥98%) were purchased from Sigma-Aldrich Química SL (Madrid, Spain). Internal standards (ISs) phenylalanine-D 5 , tryptophan-D 3 and caffeine-D 9 were purchased from CDN Isotopes (Pointe-Claire, Canada). Valproic acid (VPA) (>98%), acetaminophen (APAP) (>99%) and citric acid (CA) (>99%) were obtained from Sigma-Aldrich (San Luis, MI, USA).
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