Aoc 5000

Manufactured by Shimadzu

Sourced in Japan, Germany

The AOC-5000 is an automated sample injection system designed for use with gas chromatography (GC) instruments. It is capable of performing liquid, headspace, and solid-phase micro-extraction (SPME) sample injections. The AOC-5000 provides automated, high-precision sample handling to improve analytical reproducibility and throughput.

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Lab products found in correlation

Gc 2010, by Shimadzu (7 mentions) Gcms qp2010, by Shimadzu (5 mentions) Ms2010 plus, by Shimadzu (4 mentions) Gc 17 v3, by Shimadzu (3 mentions) Qp2010, by Shimadzu (3 mentions) Gc 17a v3, by Shimadzu (2 mentions) Class 5000 chromatography workstation software, by Shimadzu (2 mentions) Rxi 5ms capillary column, by Restek (2 mentions) Gc 2010 plus, by Shimadzu (1 mentions) Gc 2010 chromatograph, by Shimadzu (1 mentions) Db waxetr column, by Shimadzu (1 mentions) Gc ms, by Shimadzu (1 mentions) Stabilwax, by Restek (1 mentions) Rtx 5ms, by Restek (1 mentions) Pdms dvb, by Agilent Technologies (1 mentions) Stabilwax da, by Restek (1 mentions) Rtx 5ms capillary column, by Shimadzu (1 mentions) Fame mix c4 c24, by Merck Group (1 mentions) Dgu 12a, by Shimadzu (1 mentions) Sil 10 autoinjector, by Shimadzu (1 mentions)

31 protocols using aoc 5000

GC-MS Analysis of Free Fatty Acids

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The free fatty acids in the oils were analyzed by gas chromatography-mass spectrometry (QP 2010 Plus, Shimadzu, Tokyo, Japan) in a device equipped with a Split/Splitless injector (AOC-5000, Shimadzu, Tokyo, Japan) with an automatic injector (model AOC-5000). A capillary column (100 m × 0.25 mm id × 0.20 μm df, SP-2560 Supelco, Bellefonte, USA) was used as a stationary phase, with helium as the carrier gas, at a flow rate of 1.59 mL/min. The samples were injected into an injector with a Split ratio of 1:40 and a temperature of 250 °C; the pressure in the column was 300 kPa.
The oven temperature and data treatment were applied similarly to the TFA analysis, however, this analysis considered peaks with an area ≥ 0.4 % in at least two of the three replicates of each sample.
The values were shown as the percentage of the total area detected in the analysis. Since this study proposed a qualitative analysis of the free fatty acids, the values were not normalized. Therefore, the actual mean values detected during the analysis were shown.

Medeiros Vicentini-Polette C., Rodolfo Ramos P., Bernardo Gonçalves C, & Lopes De Oliveira A. (2021). Determination of free fatty acids in crude vegetable oil samples obtained by high-pressure processes. Food Chemistry: X, 12, 100166.

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Quantitative Lipid Extraction and FAME Analysis

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Total lipid was extracted from 300 mg plant samples (fresh leaves) using chloroform–methanol–phosphate buffer (1:2:0.9 v/v/v, pH 7.5; 10 mL), and fatty acids were converted to corresponding methyl esters (FAMEs) by transmethylation. For transmethylation, 1 mL of NaOH (1% v/v in methanol) was added, and mixture was incubated at 55°C for 15 min, after that 2 mL of methanolic HCl (5% v/v) was added and further incubated at 55°C for 15 min. Finally 3 mL of deionized water–hexane mixture (1:2 v/v) was added. FAMEs were extracted in three times in hexane, samples were pooled together and dried under vacuum. Dried sample was resuspended in 200 μl hexane and analyzed by using a RTX 5MS capillary column in GCMS-QP2010 (Shimadzu, Japan) coupled with an auto-sampler (AOC-5000) [6 ].

Alexander A., Singh V.K., Mishra A, & Jha B. (2019). Plant growth promoting rhizobacterium Stenotrophomonas maltophilia BJ01 augments endurance against N2 starvation by modulating physiology and biochemical activities of Arachis hypogea. PLoS ONE, 14(9), e0222405.

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Headspace Analysis of Candida and Scaptotrigona

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Headspace analysis of Candida sp. SDCP2 supernatant was performed at Small Molecules Mass Spectrometry Facility (Faculty of Arts and Sciences Harvard University) using an Agilent 6890 gas chromatograph and CTC CombiPAL autosampler. A DB-624UI column (30m x 0.32mm x 1.80um) was used, at flow rate of 2.3 mL/min, injection temperature of 220°C, interface at 220°C, ion source of 200°C, and m/z 10–500. Initial temperature was set at 30°C for 6 min, then increased at 10°C/min to 150°C, and then at 50°C/min to 250°C, and kept at 250°C for 15 min. The split ratio was 200 and headspace method adopted was 80°C for 10 min. The reads were compared to the NIST MS search 2.0 library.
Scaptotrigona depilis brood cells headspace analysis was performed at Research Support Center in Natural and Synthetic Products (NPPNS), University of São Paulo, using a Shimadzu GC-2010 Plus gas chromatograph and the auto sampler AOC 5000 with 2.5 mL syringe. A ZB-624 column (30m x 0.32mm x 1.80um) was used, at flow rate of 2.68 mL/min, injection temperature of 260°C, interface at 220°C, ion source of 200°C, and m/z 10–200. Initial temperature was set at 30°C for 6 min, then increased at 10°C/min to 150°C, and then at 50°C/min to 250°C, and kept at 250°C for 15 min. The split ratio was 5 and headspace method adopted was 80°C for 45 min. The reads were compared to the NIST 11 library.

Paludo C.R., Pishchany G., Andrade-Dominguez A., Silva-Junior E.A., Menezes C., Nascimento F.S., Currie C.R., Kolter R., Clardy J, & Pupo M.T. (2019). Microbial community modulates growth of symbiotic fungus required for stingless bee metamorphosis. PLoS ONE, 14(7), e0219696.

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GC-MS Analysis of Aldehydes

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A Shimadzu GC-2010 chromatograph was used, with a SPL1 injector splitless mode with a DB-WAX ETR column (30 m × 0.25 mm × 0.5 μm). As a detector, a Shimadzu GCMS-QP 2010 quadrupole was used, and as automatic injector, the Shimadzu AOC-5000 was selected. The quantification ion for isobutyraldehyde was 250 (m/z), that for 2-methylbutanal was 239 (m/z), for 3-metylbutanal it was 239 (m/z), for methional it was 299 (m/z) and for phenylacetaldehyde it was 297 (m/z).

Marrufo-Curtido A., Ferreira V, & Escudero A. (2022). Factors That Affect the Accumulation of Strecker Aldehydes in Standardized Wines: The Importance of pH in Oxidation. Molecules, 27(10), 3056.

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Nut Fatty Acid Profiling by GC

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Fatty acid analysis of nuts was performed using gas chromatography (GC-17 V3; Shimadzu Corporation, Kyoto, Japan) equipped with a flame ionization detector and an autosampler (AOC-5000), as described [16 (link)]. Fatty acid concentrations were expressed as percentage of the total area of all FA methyl esters (% of total fatty acid methyl esters, FAME) using GC solution software version 2.3 (Shimadzu).

Müller A.K., Helms U., Rohrer C., Möhler M., Hellwig F., Glei M., Schwerdtle T., Lorkowski S, & Dawczynski C. (2020). Nutrient Composition of Different Hazelnut Cultivars Grown in Germany. Foods, 9(11), 1596.

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GC and GC/MS Analysis of Essential Oils

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GC and GC/MS analysis were performed through flame ionization detector (FID) on Shimadzu GC 2010 gas chromatograph and QP2010 (Shimadzu Corp., Tokyo, Japan) fitted with an AOC 5000 auto-injector. The auto-injector comprises of 30 m long ZB-5 MS capillary column with 0.25 mm i.d. and 0.25 μm thick film (SGE International, Ringwood, Australia). Ten μL of EO was dissolved in 2 mL of dichloromethane with 2μL auto-injection volume in split mode. Carrier gas (nitrogen) with 1.05 mL min^-1 flow rate; oven temperature of 70 °C for 3 min and afterwards to 220 °C for 5 min at the rate of 4 °C min^-1 and injector and detector temperature was programmed at 220 °C and 250 °C, respectively. The temperature programming, injection volume, and carrier gas conditions utilized for performing GC and GC/MS were as per the procedure mentioned in German chamomile (Matricaria chamomilla L.)^{60 (link)}. For identification and recognition of compounds, a series of hydrocarbons was utilized for retention index (RI) estimation (Table 2). The EO components were identified through harmonizing the experimental RIs with the reported RIs in literature^{41 (link),61}. The components were also identified by matching the minimum mass spectral fragmentation pattern of the components with the NIST library⁶².

Rathore S., Mukhia S., Kapoor S., Bhatt V., Kumar R, & Kumar R. (2022). Seasonal variability in essential oil composition and biological activity of Rosmarinus officinalis L. accessions in the western Himalaya. Scientific Reports, 12, 3305.

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Fatty Acid Profiling by GC-MS

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Analyses by gas chromatography coupled to mass spectrometry (GC-MS) were performed in a Shimadzu/GC 2010 instrument coupled to a Shimadzu/AOC-5000 autoinjector and a mass detector (Shimadzu MS2010 Plus) with electron impact (70 eV), equipped with a DB-5MS fused silica column (Agilent J&W Advanced 30 m × 0.25 mm × 0.25 µm) (65 kPa). The parameters were as follows: split ratio 1:20, helium as carrier gas, injection volume of 1.0 μL, injector temperature of 250 °C, detector temperature at 250 °C, initial column temperature of 120 °C, remaining for 2 min, and a heating rate of 5 °C/min up to 270 °C. The total analysis time was 36 min. The identification of fatty acid esters was determined by comparing the fragmentation spectrum with those contained in the GC-MS library (MS database, NIST 5.0) [103 ].

Sales P.F., do Nascimento A.L., Pinheiro F.C., Alberto A.K., Teixeira dos Santos A.V., Carvalho H.D., de Souza G.C, & Carvalho J.C. (2023). Effect of the Association of Fixed Oils from Abelmoschus esculentus (L.) Moench, Euterpe oleracea Martius, Bixa orellana Linné and Chronic SM® on Atherogenic Dyslipidemia in Wistar Rats. Molecules, 28(18), 6689.

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Metabolomic Profiling of Plant Samples

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About 100 mg of fresh samples was ground into a fine powder using a mortar and pestle. Metabolites were extracted with ice-cold methanol (2 mL, 70%, v/v) and further analysis of extracted metabolites was conducted by liquid chromatography–mass spectrometry. The background of each spectrum was subtracted, and the data were smoothed and then centered using Mass Lynx software version 4.1 (Micromass, Waters, Milford, MA, USA). Peaks were identified by comparison with the online METLIN database.
Similar to the above method, about 100 mg fine powdered sample was extracted with 100% ice-cold methanol. Adonitol (0.2 mg/mL) was used as an internal reference for quantification. Samples were prepared and analyzed by a gas chromatography–mass spectrometry instrument (GC-MS, Shimadzu, Kyoto City, Japan; Modal GC-MS TQ8040) equipped with an autosampler (AOC-5000, Shimadzu, Japan) and flame ionization detection (FID)/capillary column [22 (link)]. Mass spectra were recorded, and metabolites were identified by comparison with the NIST library. The concentration was measured with respect to the internal reference peak area and expressed as μg/g fresh weight.

Tanna B., Yadav S., Patel M.K, & Mishra A. (2024). Metabolite Profiling, Biological and Molecular Analyses Validate the Nutraceutical Potential of Green Seaweed Acrosiphonia orientalis for Human Health. Nutrients, 16(8), 1222.

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GC-MS Analysis of Chloroform Residue

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Chloroform residue was measured by a headspace autosampler AOC-5000 connected to the GCMS-QP2010 Ultra device (Shimadzu, Kyoto, Japan) equipped with a fused silica capillary column (Stabilwax, 30 m × 0.25 mm × 0.25 µm, Restek, PA, USA). Headspace samples were prepared in 20 mL gas tied vials filled with cca 0.05 mg of sample for an equilibrium time of 30 min at 95 °C. Helium was used as the carrier gas, at a flow rate of 1.26 mL/min. The temperature of the injection was maintained at 230 °C at a split ratio of 1:20, the volume of the injected sample equalling 1 mL. The temperature of the column was held at 50 °C for 1 min, and then increased to 70 °C at a rate of 25 °C/min, and increased to 240 °C for additional 2 min. The temperatures of the ion source (EI, 70 eV) and the interface were set at 200 °C and 240 °C, respectively. The range of the scan was 25–250 (m/z) at event time 0.3. The peaks obtained in the resulting TIC spectrum were identified with the help of the NIST11 Spectra Library.

Amini Moghaddam M., Di Martino A., Šopík T., Fei H., Císař J., Pummerová M, & Sedlařík V. (2021). Polylactide/Polyvinylalcohol-Based Porous Bioscaffold Loaded with Gentamicin for Wound Dressing Applications. Polymers, 13(6), 921.

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Analysis of Hop Volatiles by GC-MS

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The analysis of volatile compounds of hop leaves and cones samples after supercritical fluid extraction was performed using GC-2010 (Shimadzu, Kyoto, Japan) gas chromatography system with AOC-5000 (Shimadzu, Kyoto, Japan) autoinjector and GCMS-QP2010 mass-spectrometry detector (Shimadzu, Kyoto, Japan). Separation was carried out using Rtx-5MS (Restek, Bellefonte, PA, USA) column (30 m × 0.25 mm × 0.25 µm) and helium as the carrier gas. The analysis was performed under the following conditions: injector temperature 240 °C; split ratio 1:10; injection volume 1 µL; and flow rate 1.2 mL/min. The column oven temperature was programmed from 60 °C (held for 3 min), heated up to 150 °C at the speed of 2 °C/min, held at 150 °C for 5 min and then heated to 285 °C at the speed of 10 °C/min (held for 8 min). Mass spectrometry detection was performed using electron impact (EI) ionization using 200 °C ion source temperature, 70 eV ionization energy, and 30–400 m/z scan range. Volatile compounds were identified using NIST05 (NIST, Gaithersburg, MD, USA) mass spectra library and by calculating RI (retention indices), which were calculated using n-alkanes (C₈–C₂₀) retention times for the same analysis conditions as for the samples.

Stanius Ž., Dūdėnas M., Kaškonienė V., Stankevičius M., Skrzydlewska E., Drevinskas T., Ragažinskienė O., Obelevičius K, & Maruška A. (2022). Analysis of the Leaves and Cones of Lithuanian Hops (Humulus lupulus L.) Varieties by Chromatographic and Spectrophotometric Methods. Molecules, 27(9), 2705.

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