Lambda 25 uv

Manufactured by PerkinElmer

Sourced in Italy, United States

The PerkinElmer Lambda 25 UV is a double-beam UV-Vis spectrophotometer designed for routine analysis and research applications. It features a wavelength range of 190 to 1,100 nanometers and can measure absorbance, transmittance, and reflectance. The instrument is equipped with a halogen and deuterium lamp for a wide spectral coverage.

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Lab products found in correlation

Winlab v6, by PerkinElmer (1 mentions) Lambda 25 uv vis, by PerkinElmer (1 mentions) Originpro, by OriginLab (1 mentions) Zetasizer nano z, by Malvern Panalytical (1 mentions) Evo 40, by Zeiss (1 mentions) X pert pro, by Malvern Panalytical (1 mentions)

Close spelling variants of this product

UV–vis spectrometer model lambda 25 Lambda 25 UV/Visible spectrophotometer Lambda 25 UV/vis absorption spectrophotometer Lambda 25 UV spectrophotometer Lambda 25 UV/VIS Lambda 25 UV-Vis spectrophotometer Lambda 25 UV-visible Precisely Lambda 25 UV/Vis Spectrometer Lambda 25 UV/VIS Spectrometer LAMBDA 25 UV/Vis Systems

9 protocols using lambda 25 uv

Firocoxib Solubility and Dissolution Evaluation

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Firocoxib solubility was determined using the shake-flask method in deionized water at 25 °C. An aliquot of the saturated solution was filtered (0.45 µm, Millipore). The drug concentration was determined by spectrophotometric detection at 275 nm (Lambda 25 UV-Vis, Perkin-Elmer, Monza, Italy) after 2 h (considered as the average transit time to reach the small bowel, which is the site of absorption [15 (link),16 ]) and after 24–48 h to evaluate the equilibrium solubility [17 (link)].
Samples of firocoxib, loaded fibers (-el), physical mixtures (-pm), and the commercial product were evaluated in in vitro dissolution tests using the USP Apparatus 2 (Erweka DT-D6, Dusseldorf, Germany) paddle with a rotation speed of 100 rpm in 1000 mL of deionized water at 37 °C. The concentrations of the dissolved drug were determined on a filtered portion of the dissolution medium by UV detection at 275 nm (Lambda 25 UV, Perkin-Elmer, Monza, Italy). The data were processed through suitable software (Winlab V6 software, Perkin-Elmer, Monza, Italy) to obtain the dissolution profiles. All samples contained a dose of 57 mg of firocoxib and for each sample, six replicates were performed.

Maggi L., Friuli V., Chiesa E., Pisani S., Sakaj M., Celestini P, & Bruni G. (2019). Improvement of the Firocoxib Dissolution Performance Using Electrospun Fibers Obtained from Different Polymer/Surfactant Associations. International Journal of Molecular Sciences, 20(12), 3084.

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Characterization of Nanomaterials via Spectroscopy

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Perkin-Elmer lambda 25 UV–vis spectrometer was employed for UV–vis absorption measurement in the range of 200–800 nm. The morphology and shape of NPLs were studied using a field-emission scanning electron microscope (Supra 400VP, Zeiss, Oberkochen, Germany). Dynamic light scattering experiments were carried out at room temperature using a Zetasizer Nano-ZS90 Malvern. The elementary compositions of the samples were determined using EDS on a Tescan energy-dispersive spectrometer. The ImageJ software was used to determine the gray intensity of the blue color of the paper-based microfluidic device.

Pebdeni A.B., AL-Baiati M.N, & Hosseini M. (2024). New application of bimetallic Ag/Pt nanoplates in a colorimetric biosensor for specific detection of E. coli in water. Beilstein Journal of Nanotechnology, 15, 95-103.

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Enzyme Kinetics with Competitive Inhibitors

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The inhibition assays were conducted in a buffer system containing 50 mM Tris-HCl, 200 mM NaCl, 5% ν/ν glycerol (pH 8.0) at room temperature. The reaction was initiated by adding 1 µM enzyme to the mixture containing 1 mM L-ascorbate and L-Trp (20 – 3200 µM) in the absence or presence of probe 1 or 2 at three different concentrations. Steady-state formation of NFK was monitored at 321 nm (extinction coefficient, 3752 M⁻¹cm⁻¹) using a Perkin Elmer Lambda 25 UV-visible spectrophotometer.
The data were analyzed using OriginPro (OriginLab, Northampton, Massachusetts, USA). Global fittings were conducted across the datasets using the following equation:

\frac{1}{ν_{0}} = [(1 + \frac{[I]}{K_{i} 1}) \frac{K_{m}}{k_{cat}}] \frac{1}{[S]} + (1 + \frac{[I]}{K_{i} 2}) \frac{1}{k_{cat}}

in which parameters, k_cat, K_m, K_i1, and K_i2 are shared.

Shin I., Ambler B.R., Wherritt D., Griffith W.P., Maldonado A.C., Altman R.A, & Liu A. (2018). Stepwise O atom Transfer in Heme-Based Tryptophan Dioxygenase: Role of Substrate Ammonium in Epoxide Ring Opening. Journal of the American Chemical Society, 140(12), 4372-4379.

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Detailed Spectroscopic Analysis of Color

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Visible spectra in the range 330–650 nm at 5 nm intervals were obtained using a PerkinElmer LAMBDA 25 UV–vis spectrophotometer (Waltham, MA, USA). Quartz cuvettes of 1 mm thickness were used. Color analysis was performed by measurements at 420 nm, 520 nm, and 620 nm to calculate color intensity (CI) as the sum of these absorbances as defined by Glories, 1984 [83 (link)]. The CIELab parameters were calculated using the “OIV-MA-AS2-11 Method: Determination of chromatic characteristics according to CIELab” [84 ] (OIV, 2006). These parameters were: L*, describing black to white lightness; b*, blue to yellow; a*, red to green; C*, chroma or saturation; and H*, slant angle. The total polyphenol index (TPI) was analyzed by measuring the absorbance of the sample (1:25 pre-dilution) at 280 nm in a 10 mm quartz cell using the Ribereau-Gayon, 1970 [85 ] method.

Carrasco-Quiroz M., del Alamo-Sanza M., Martínez-Gil A.M., Sánchez-Gómez R., Martínez-Martínez V, & Nevares I. (2023). Influence of Oxygen Management on Color and Phenolics of Red Wines. Molecules, 28(1), 459.

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UV-Vis Spectroscopy Protocol

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All UV–vis measurements were recorded on a PerkinElmer Lambda
25 UV–vis spectrometer, using water as a reference.

Koch A.H., Morsbach S., Bereau T., Lévêque G., Butt H.J., Deserno M., Landfester K, & Fytas G. (2020). Probing Nanoparticle/Membrane Interactions by Combining Amphiphilic Diblock Copolymer Assembly and Plasmonics. The Journal of Physical Chemistry. B, 124(5), 742-750.

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Characterization of Synthesized Gold Nanoparticles

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The extinction profile
for the synthesized HBF_GNPs was obtained from the UV–visible
absorption spectra performed using the LAMBDA 25 UV–visible
spectrometer, PerkinElmer. Spectra were obtained by performing a scan
from 200 to 700 nm in a 1 mL quartz cuvette of 1 cm path length. The
gold colloids were diluted with 100 mM potassium phosphate buffer
of pH 7.4 before performing the scans.
The size and morphological
features of the HBF_GNPs and SAED pattern were obtained by performing
electron microscopy using the transmission electron microscope, JEOL
2100F, with an incident energy of 200 keV. Prior to viewing, the GNPs
were concentrated by centrifugation of the colloidal gold solutions,
and the concentrated samples were drop-casted onto copper grids of
300 mesh size.
The crystallinity of the HBF_GNPs was investigated
using X-ray
diffraction (PANalytical X’pert PRO) with Cu Kα radiation
(λ = 1.54 Å). For this, thin films of HBF_GNPs were prepared
on clean glass slides by repeated drop-casting. The elemental analysis
of GNPs was performed using the scanning electron microscope coupled
with energy-dispersive X-ray spectroscopy (Zeiss EVO40). Zeta potential
and particle size analysis using DLS were carried out with Zetasizer
Nano Z from Malvern Panalytical.

Madhavan A.A., Juneja S., Moulick R.G, & Bhattacharya J. (2020). Growth Kinetics of Gold Nanoparticle Formation from Glycated Hemoglobin. ACS Omega, 5(8), 3820-3827.

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UV-Vis Spectroscopy of Printed Polymer

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UV/vis spectrum of the printed
polymer was measured from 890 to 300 nm using a PerkinElmer Lambda
25 UV/vis spectrophotometer. The analyzed NLO lens used as a sample
was placed in front of the sample cuvette holder, and the reference
cuvette holder was left empty as air was used as reference. A slit
of 1.0 nm was used, with a scan speed of 240 nm/min and a data interval
of 5.0 nm.

Kukkonen E., Lahtinen E., Myllyperkiö P., Konu J, & Haukka M. (2018). Three-Dimensional Printing of Nonlinear Optical Lenses. ACS Omega, 3(9), 11558-11561.

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UV-Vis Analysis of Biogenic AgNPs

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The reduction in pure silver ions was recorded by measuring the UV–Vis spectrum of the synthesized AgNP’s of G. crassa at room temperature with a Perkin Elmer Lambda 25 UV–Vis spectrometer at the wavelength of 200–800 nm [28 ].

Lavakumar V., Masilamani K., Ravichandiran V., Venkateshan N., Saigopal D.V., Ashok Kumar C.K, & Sowmya C. (2015). Promising upshot of silver nanoparticles primed from Gracilaria crassa against bacterial pathogens. Chemistry Central Journal, 9, 42.

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Enzymatic Activity Characterization of BChE

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Enzyme activity was measured on a Perkin Elmer Lambda 25 UV/VIS spectrophotometer according to the method of Ellman et al. (1961) as described earlier. Here, the substrate butyrylthiocholine (BTCh) was hydrolyzed by BChE, and thiocholine was released. The free thiocholine then reacted with 5,5′-dithio-2-bis-nitrobenzoate (DTNB) and produced the thionitrobenzoate (TNB) anion which absorbed light at 412 nm. For the physicochemical characterization of horse serum BChE, optimum pH, optimum temperature, energy of activation (Ea) and temperature coefficient (Q10) were determined accordingly (Segel, 1975) .

Dalmizrak O., Teralı K., Yetkin O., Ogus I.H, & Ozer N. (2019). Computational and experimental studies on the interaction between butyrylcholinesterase and fluoxetine: implications in health and disease. Xenobiotica; the fate of foreign compounds in biological systems, 49(7).

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