GSH-Px activity in blood hemolysates was determined spectrophotometrically with an automated biochemical analyser RX-Daytona (Randox Laboratories, Crumlin, UK) using the commercial Ransel kit (Randox Laboratories, Crumlin, UK), which is based on the method of Paglia and Valentine (1967 (link)). Blood hemolysates were diluted 41-fold before being analyzed with Ransel Diluent (Diluting agent, Randox Laboratories, Crumlin, UK). GSH-Px activity was expressed as units per gram of hemoglobin (U/g Hgb). SOD activity in blood hemolysates was determined spectrophotometrically with an automatic biochemical analyser RX Daytona (Randox Laboratories, Crumlin, UK), using a commercially available Ransod kit (Randox Laboratories, Crumlin, UK), which is based on the original method of McCord and Fridovich (1969 (link)). Before the analyses, samples of hemolysates were diluted 1:200 with the Ransod Sample Diluent (0.01 mmol/L phosphate buffer, pH 7.0; Randox Laboratories, Crumlin, UK). The activity was expressed as U/g Hgb. Hemoglobin concentration was determined by the cyanmethemoglobin method using an automated hematological analyser ADVIA 120 (Siemens, Munich, Germany) (Paglia and Valentine, 1967 (link)).
Rx daytona
Manufactured by Randox
Sourced in United Kingdom, United States, Ireland, Germany
The RX Daytona is a fully automated clinical chemistry analyzer designed for high-throughput testing in medical laboratories. It is capable of performing a wide range of routine clinical chemistry tests efficiently and accurately.
Automatically generated - may contain errors
Lab products found in correlation
Advia 120, by Siemens (7 mentions)
Evidence investigator, by Randox (6 mentions)
Ransod kit, by Randox (3 mentions)
Ransel kit, by Randox (3 mentions)
Ilyte, by Werfen (3 mentions)
Heraeus primo r, by Thermo Fisher Scientific (3 mentions)
Ransel diluent, by Randox (2 mentions)
Ransod sample diluent, by Randox (2 mentions)
Ba 88a, by Mindray (2 mentions)
Nefa kit, by Randox (2 mentions)
Plus blood collection tubes, by BD (2 mentions)
Cobas 702, by Roche (2 mentions)
Cobas 602, by Roche (2 mentions)
Evidence investigator metabolic syndrome array, by Randox (2 mentions)
Au680 chemistry analyser, by Beckman Coulter (2 mentions)
Adiponectin, by R&D Systems (2 mentions)
Au640, by Olympus (1 mentions)
Abx micros esv 60, by Horiba (1 mentions)
Vacutainer, by BD (1 mentions)
Tas kit, by Randox (1 mentions)
97 protocols using rx daytona
1
Antioxidant Enzyme Activities in Blood
2
Spectrophotometric Glutathione Peroxidase Assay
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Glutathione peroxidase activity was measured spectrophotometrically with an automated biochemistry analyser RX Daytona (Randox, Crumlin, Great Britain) using a commercial Ransel kit (Randox, Crumlin, Great Britain), which is based on the method of Paglia and Valentine [48 (link)]. According to the method, GPX activity is determined indirectly by measuring the rate of formation of oxidized glutathione. GPX activity was expressed as units per gram of haemoglobin (U/g Hgb). Haemoglobin concentration in the whole blood haemolysates was determined spectrophotometrically by the cyano-methaemoglobin method using an automated biochemistry analyser RX Daytona (Randox, Crumlin, Great Britain).
3
Enzymatic Antioxidant Activities in Whole Blood
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Activity of GPx in whole blood hemolysates was determined spectrophotometrically with an automated biochemical analyzer RX-Daytona (Randox, Crumlin, UK) using the commercial Ransel kit (Randox Laboratories, Crumlin, UK), which is based on the method of Paglia and Valentine (1967) . Blood hemolysates were diluted 41-fold before analyses with Ransel Diluent (Diluting agent, Randox Laboratories, Crumlin, UK). Activity of GPx was expressed as units per gram of hemoglobin (U/g Hgb). Hemoglobin concentration was determined by the cyanmethemoglobin method using automated hematological analyzer ADVIA 120 (Siemens, Munich, Germany) (Paglia and Valentine, 1967 ). SOD activity in whole blood hemolysates was determined spectrophotometrically with an automatic biochemical analyzer RX Daytona (Randox Laboratories), using commercially available Ransod kit (Randox Laboratories), which is based on the original method of McCord and Fridovich (1969) . Before analyses samples of hemolysates were diluted 1:200 with Ransod Sample Diluent (0.01 mmol/L phosphate buffer, pH 7.0; Randox Laboratories). Activity was expressed as U/g Hgb. Hemoglobin concentration was determined by the cyanmethemoglobin method using automated hematological analyzer ADVIA 120 (Siemens, Munich, Germany) (McCord and Fridovich, 1969 ).
4
Comprehensive Metabolic Profiling of T2DM
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Blood samples were drawn following an overnight fast. Serum glucose was measured using hexokinase methodology in an automated analyser (Olympus AU640; Olympus, Germany/Rx Daytona, Randox Laboratories, Wülfrath, Germany). Serum total, low-density lipoprotein- and high-density lipoprotein-cholesterol and trigaclycerol concentrations were collected in serum tubes and measured using enzymatic reagents (Olympus AU640; Olympus, Germany/Rx Daytona, Randox Laboratories). In T2DM subjects, serum insulin was measured using an automated monoclonal antibody-based two-site immunoenzymometric assay (AIA-1800 system; Tosoh Europe NV, Tessenderlo, Belgium), and glycosylated haemoglobin was measured with an automated HPLC instrument-reagent system (model HLC-723 G7; Tosoh Europe NV).
5
Biochemical Evaluation in Fasted Mice
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Mice abstained for 12 h with a constant water supply before being ethically sedated with diethyl ether. Blood was obtained from the orbital venous plexus in plain tubes and centrifuged at 2500 rpm for 15 min. The sera were separated and kept at 4 °C before direct evaluation of AST, ALT, ALP, Total bilirubin (TBIL), Lactate dehydrogenase (LDH), Total protein (TP), Glucose (GLU), Triglycerides (TG), Cholesterol (CHOL), High-density lipoprotein cholesterol (HDL-C), Low-density lipoprotein cholesterol (LDL-C), and Urea (BUN) (Abou-Kassem et al., 2021 , Reda et al., 2021 ). Analysis was adopted via an automated clinical chemistry analyzer (RX DaytonaTM; Randox Laboratories, Crumlin, County Antrim, UK).
6
Automated Clinical Chemistry Analysis
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Analysis was carried out using an automated clinical chemistry analyzer (RX Daytona TM ; Randox Laboratories, Crumlin, County Antrim, UK) using the Randox range of biochemistry reagents. As a standard practice, operation of the analyzer was verified by running standard controls prior and post sample analyses. All the values obtained from the standard control for the different biochemical parameters were always within the expected ranges. An External Quality Assurance System (EQAS) program was used periodically to assess the analytical performance of the instrument. Every single crucial precautionary measure such as sampling time, randomization and storage conditions were harmonized to rule out pre-analytical, analytical and post-analytical variations. Different methodologies using the RX Daytona TM automated analyzer for analysis of different analytes are listed in Table 1.
7
Comprehensive Metabolic Biomarker Assessment
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A clinical bioanalyser (RX Daytona; Randox Laboratories) was used to measure levels of glucose, TAG and total and HDL-cholesterol in serum samples (19) . LDL-cholesterol levels were calculated as (Total cholesterol/HDL-cholesterol) -(TAG/2•2). Insulin, leptin and TNFα levels were measured using a biochip array system (Evidence Investigator; Randox Laboratories). Adiponectin levels were measured using ELISA (ALPCO Diagnostics kit; ALPCO) and homocysteine levels using a flourescence polarisation immunoassay. A detailed description of the lipid extraction methodology and fatty acid analysis has been outlined elsewhere (20) . The National Cholesterol Education Programme's Adult Treatment Panel III criterion was applied to evaluate risk for the MetS (21) .
8
Comprehensive Blood Lactate and Glucose Measurement
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Tubes containing EDTA and sodium fluoride were used for blood sampling. EDTA plasma was used for l -lactate and d -lactate, and plasma from sodium fluoride tubes were used for glucose analysis. The plasma l -lactate (levogyre) and glucose were determined using a commercial enzymatic kit (l -lactate-LAC and Randox Glucose GOD-PAP, respectively; Randox®, Crumlin, UK) in an automatic biochemical analyzer (Rx Daytona; Randox®). Further, a d -lactate (dextrogyre) enzyme assay (BioVision®, Milpitas, CA, USA) was used to measure the d -lactate plasma concentration.
9
Plasma Lipid and Glucose Analysis
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10
Comprehensive Biomarker Analysis in Animal Study
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At the end of the study, blood samples were collected for haematology, clinical pathology investigations and biomarker analysis. The haematology analyzer (model ABX Micros ESV 60; HORIBA Instruments Inc., Irvine, CA, USA) was used for haematological evaluation. Clinical chemistry parameters were estimated using the RX Daytona+ (Randox Laboratories, Kearneysville, WV, USA) instrument. Commercially available enzyme-linked immunosorbent assay kits (KinesisDx kits; Krishgen Biosystems, Mumbai, India) were used to quantify insulin, leptin, adiponectin and free fatty acids. At the end of the study, all animals were subjected to a detailed gross necropsy examination. External body surfaces, orifices and cavities such as abdominal, thoracic and cranial were included in the examination.
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