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Rx daytona

Manufactured by Randox
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Sourced in United Kingdom, United States, Ireland, Germany

The RX Daytona is a fully automated clinical chemistry analyzer designed for high-throughput testing in medical laboratories. It is capable of performing a wide range of routine clinical chemistry tests efficiently and accurately.

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Lab products found in correlation

Advia 120, by Siemens (7 mentions) Evidence investigator, by Randox (6 mentions) Ransod kit, by Randox (3 mentions) Ransel kit, by Randox (3 mentions) Ilyte, by Werfen (3 mentions) Heraeus primo r, by Thermo Fisher Scientific (3 mentions) Ransel diluent, by Randox (2 mentions) Ransod sample diluent, by Randox (2 mentions) Ba 88a, by Mindray (2 mentions) Glucose kit, by Randox (2 mentions) Nefa kit, by Randox (2 mentions) Lactate kit, by Randox (2 mentions) Plus blood collection tubes, by BD (2 mentions) Au680 chemistry analyser, by Beckman Coulter (2 mentions) Cobas 602, by Roche (2 mentions) Cobas 702, by Roche (2 mentions) Evidence investigator metabolic syndrome array, by Randox (2 mentions) Luminex, by Bio-Rad (2 mentions) Adiponectin, by R&D Systems (2 mentions) Abx micros esv 60, by Horiba (1 mentions)

95 protocols using rx daytona

1

Antioxidant Enzyme Activities in Blood

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GSH-Px activity in blood hemolysates was determined spectrophotometrically with an automated biochemical analyser RX-Daytona (Randox Laboratories, Crumlin, UK) using the commercial Ransel kit (Randox Laboratories, Crumlin, UK), which is based on the method of Paglia and Valentine (1967 (link)). Blood hemolysates were diluted 41-fold before being analyzed with Ransel Diluent (Diluting agent, Randox Laboratories, Crumlin, UK). GSH-Px activity was expressed as units per gram of hemoglobin (U/g Hgb). SOD activity in blood hemolysates was determined spectrophotometrically with an automatic biochemical analyser RX Daytona (Randox Laboratories, Crumlin, UK), using a commercially available Ransod kit (Randox Laboratories, Crumlin, UK), which is based on the original method of McCord and Fridovich (1969 (link)). Before the analyses, samples of hemolysates were diluted 1:200 with the Ransod Sample Diluent (0.01 mmol/L phosphate buffer, pH 7.0; Randox Laboratories, Crumlin, UK). The activity was expressed as U/g Hgb. Hemoglobin concentration was determined by the cyanmethemoglobin method using an automated hematological analyser ADVIA 120 (Siemens, Munich, Germany) (Paglia and Valentine, 1967 (link)).
Leskovec J., Levart A., Nemec Svete A., Perić L., Đukić Stojčić M., Žikić D., Salobir J, & Rezar V. (2018). Effects of supplementation with α-tocopherol, ascorbic acid, selenium, or their combination in linseed oil-enriched diets on the oxidative status in broilers. Poultry Science, 97(5), 1641-1650.
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2

Spectrophotometric Glutathione Peroxidase Assay

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Glutathione peroxidase activity was measured spectrophotometrically with an automated biochemistry analyser RX Daytona (Randox, Crumlin, Great Britain) using a commercial Ransel kit (Randox, Crumlin, Great Britain), which is based on the method of Paglia and Valentine [48 (link)]. According to the method, GPX activity is determined indirectly by measuring the rate of formation of oxidized glutathione. GPX activity was expressed as units per gram of haemoglobin (U/g Hgb). Haemoglobin concentration in the whole blood haemolysates was determined spectrophotometrically by the cyano-methaemoglobin method using an automated biochemistry analyser RX Daytona (Randox, Crumlin, Great Britain).
Nemec Svete A., Verk B., Čebulj-Kadunc N., Salobir J., Rezar V, & Domanjko Petrič A. (2021). Inflammation and its association with oxidative stress in dogs with heart failure. BMC Veterinary Research, 17, 176.
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3

Enzymatic Antioxidant Activities in Whole Blood

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Activity of GPx in whole blood hemolysates was determined spectrophotometrically with an automated biochemical analyzer RX-Daytona (Randox, Crumlin, UK) using the commercial Ransel kit (Randox Laboratories, Crumlin, UK), which is based on the method of Paglia and Valentine (1967) . Blood hemolysates were diluted 41-fold before analyses with Ransel Diluent (Diluting agent, Randox Laboratories, Crumlin, UK). Activity of GPx was expressed as units per gram of hemoglobin (U/g Hgb). Hemoglobin concentration was determined by the cyanmethemoglobin method using automated hematological analyzer ADVIA 120 (Siemens, Munich, Germany) (Paglia and Valentine, 1967 ). SOD activity in whole blood hemolysates was determined spectrophotometrically with an automatic biochemical analyzer RX Daytona (Randox Laboratories), using commercially available Ransod kit (Randox Laboratories), which is based on the original method of McCord and Fridovich (1969) . Before analyses samples of hemolysates were diluted 1:200 with Ransod Sample Diluent (0.01 mmol/L phosphate buffer, pH 7.0; Randox Laboratories). Activity was expressed as U/g Hgb. Hemoglobin concentration was determined by the cyanmethemoglobin method using automated hematological analyzer ADVIA 120 (Siemens, Munich, Germany) (McCord and Fridovich, 1969 ).
Pirman T., Rezar V., Vrecl M., Salobir J, & Levart A. (2021). Effect of Olive Leaves or Marigold Petal Extract on Oxidative Stress, Gut Fermentative Activity, and Mucosa Morphology in Broiler Chickens Fed a Diet Rich in n-3 Polyunsaturated Fats. The Journal of Poultry Science, 58(2), 119-130.
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4

Comprehensive Metabolic Profiling of T2DM

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Blood samples were drawn following an overnight fast. Serum glucose was measured using hexokinase methodology in an automated analyser (Olympus AU640; Olympus, Germany/Rx Daytona, Randox Laboratories, Wülfrath, Germany). Serum total, low-density lipoprotein- and high-density lipoprotein-cholesterol and trigaclycerol concentrations were collected in serum tubes and measured using enzymatic reagents (Olympus AU640; Olympus, Germany/Rx Daytona, Randox Laboratories). In T2DM subjects, serum insulin was measured using an automated monoclonal antibody-based two-site immunoenzymometric assay (AIA-1800 system; Tosoh Europe NV, Tessenderlo, Belgium), and glycosylated haemoglobin was measured with an automated HPLC instrument-reagent system (model HLC-723 G7; Tosoh Europe NV).
Breen C., Ryan M., McNulty B., Gibney M.J., Canavan R, & O'Shea D. (2014). High saturated-fat and low-fibre intake: a comparative analysis of nutrient intake in individuals with and without type 2 diabetes. Nutrition & Diabetes, 4(2), e104-.
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5

Comprehensive Metabolic Biomarker Assessment

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A clinical bioanalyser (RX Daytona; Randox Laboratories) was used to measure levels of glucose, TAG and total and HDL-cholesterol in serum samples (19) . LDL-cholesterol levels were calculated as (Total cholesterol/HDL-cholesterol) -(TAG/2•2). Insulin, leptin and TNFα levels were measured using a biochip array system (Evidence Investigator; Randox Laboratories). Adiponectin levels were measured using ELISA (ALPCO Diagnostics kit; ALPCO) and homocysteine levels using a flourescence polarisation immunoassay. A detailed description of the lipid extraction methodology and fatty acid analysis has been outlined elsewhere (20) . The National Cholesterol Education Programme's Adult Treatment Panel III criterion was applied to evaluate risk for the MetS (21) .
Lenighan Y.M., Nugent A.P., Li K.F., Brennan L., Walton J., Flynn A., Roche H.M, & McNulty B.A. (2017). Processed red meat contribution to dietary patterns and the associated cardio-metabolic outcomes. The British journal of nutrition, 118(3).
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6

Comprehensive Blood Lactate and Glucose Measurement

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Tubes containing EDTA and sodium fluoride were used for blood sampling. EDTA plasma was used for l-lactate and d-lactate, and plasma from sodium fluoride tubes were used for glucose analysis. The plasma l-lactate (levogyre) and glucose were determined using a commercial enzymatic kit (l-lactate-LAC and Randox Glucose GOD-PAP, respectively; Randox®, Crumlin, UK) in an automatic biochemical analyzer (Rx Daytona; Randox®). Further, a d-lactate (dextrogyre) enzyme assay (BioVision®, Milpitas, CA, USA) was used to measure the d-lactate plasma concentration.
Minami N.S., Sousa R.S., Oliveira F.L., Dias M.R., Cassiano D.A., Mori C.S., Minervino A.H, & Ortolani E.L. (2020). Subacute Ruminal Acidosis in Zebu Cattle: Clinical and Behavioral Aspects. Animals : an Open Access Journal from MDPI, 11(1), 21.
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7

Plasma Lipid and Glucose Analysis

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Clinical chemistry analysis was performed using a RxDaytona™ chemical analyser autoanalyser (Randox Laboratories, Crumlin, UK) and Randox reagents. The plasma lipid profile, glucose and insulin were measured as previously described [15 (link)].
Jackson K.G., Li Y., Ryan M.F., Gibney E.R., Brennan L., Roche H.M., Williams C.M., Lovegrove J.A, & Vimaleswaran K.S. (2016). Association of the tumor necrosis factor-alpha promoter polymorphism with change in triacylglycerol response to sequential meals. Nutrition Journal, 15, 70.
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8

Comprehensive Biomarker Analysis in Animal Study

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At the end of the study, blood samples were collected for haematology, clinical pathology investigations and biomarker analysis. The haematology analyzer (model ABX Micros ESV 60; HORIBA Instruments Inc., Irvine, CA, USA) was used for haematological evaluation. Clinical chemistry parameters were estimated using the RX Daytona+ (Randox Laboratories, Kearneysville, WV, USA) instrument. Commercially available enzyme-linked immunosorbent assay kits (KinesisDx kits; Krishgen Biosystems, Mumbai, India) were used to quantify insulin, leptin, adiponectin and free fatty acids. At the end of the study, all animals were subjected to a detailed gross necropsy examination. External body surfaces, orifices and cavities such as abdominal, thoracic and cranial were included in the examination.
Shanmugham V, & Subban R. (2022). Comparison of the Anti-Obesity Effect of Enriched Capsanthin and Capsaicin from Capsicum annuum L. Fruit in Obesity-Induced C57BL/6J Mouse Model. Food Technology and Biotechnology, 60(2), 202-212.
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9

Lipid Biomarker Measurement Protocol

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Plasma cholesterol, LDL cholesterol, apo B-100, apo A1, small dense LDL, and triglyceride assays were all perfomed on the RX Daytona clinical analyser (Randox, Laboratories Ltd., Antrim, UK). All assays were performed with authentic standards and controls.
Graham D.L., Lorenz M., Young A.J, & Lowe G.M. (2017). A Possible Indicator of Oxidative Damage in Smokers: (13Z)-Lycopene?. Antioxidants, 6(3), 69.
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10

Hematological and Biochemical Profiling Protocol

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Hematological parameters including the red blood cell (RBC), the white blood cell (WBC), hemoglobin (Hb), monocytes, lymphocytes, eosinophils, basophils, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), hematocrit (HCT), mean cell hemoglobin concentration (MCHC), and platelets count were analyzed with the help of an automated hematology analyzer (Sysmax-1800i, Japan). Biochemical parameters of glucose, cortisol, creatinine, urea, low-density lipoproteins (LDL), high-density lipoproteins (HDL), total protein, and albumin were analyzed by using a fully automated Clinical chemistry analyzer (Rx Daytona, Randox, United Kingdom). MCV, MCH, and MCHC were calculated using the following equations: MCV(fl)=(Hct×10)RBC
MCH(pg)=Hb(in g/L)RBC(in millions/μL)
MCHC(g/dL)=(Hb×10)Hct
Almanaa T.N., Aref M., Kakakhel M.A., Elshopakey G.E., Mahboub H.H., Abdelazim A.M., Kamel S., Belali T.M., Abomughaid M.M., Alhujaily M., Fahmy E.M., Ezzat Assayed M., Mostafa-Hedeab G, & Daoush W.M. (2022). Silica Nanoparticle Acute Toxicity on Male Rattus norvegicus Domestica: Ethological Behavior, Hematological Disorders, Biochemical Analyses, Hepato-Renal Function, and Antioxidant-Immune Response. Frontiers in Bioengineering and Biotechnology, 10, 868111.
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