The following antibodies and DAPI were used: SSEA4-FITC (BioLegend, San Diego, CA, United States), TRA-1-81-PE (Thermo Fisher Scientific, Waltham, MA, United States), TRA-1-60-PE (Thermo Fisher Scientific, Waltham, MA, United States), E-cadherin-Alexa Flour 647 (BD, Franklin Lakes, NJ, United States), and EPCAM-APC (BD, Franklin Lakes, NJ, United States). The cells were incubated with antibody on ice for 30 min then washed twice. The cells were analyzed in FACS Jazz (BD, Franklin Lakes, NJ, United States) and FlowJo v. 7.6.1 (FlowJo LCC, Ashland, CO, United States).
Tra 1 60 pe
Manufactured by Thermo Fisher Scientific
Sourced in United States
The TRA-1-60-PE is a laboratory instrument used for the detection and quantification of specific target molecules or cells in a sample. It is designed to provide reliable and accurate results for applications in fields such as cell biology, immunology, and flow cytometry. The core function of the TRA-1-60-PE is to analyze samples and generate data based on the presence and characteristics of the target analytes.
Automatically generated - may contain errors
Lab products found in correlation
Ssea4 fitc, by BioLegend (1 mentions)
Epcam apc, by BD (1 mentions)
Facsjazz, by BD (1 mentions)
Cd31 alexa fluor 647, by BioLegend (1 mentions)
Cd34 pe, by Thermo Fisher Scientific (1 mentions)
Cd73 pe, by Thermo Fisher Scientific (1 mentions)
Cd90 pe, by Thermo Fisher Scientific (1 mentions)
Cell cycle assay solution deep red, by Dojindo Laboratories (1 mentions)
Cd105 apc, by Thermo Fisher Scientific (1 mentions)
Cd44 pe, by Thermo Fisher Scientific (1 mentions)
Annexin 5 fitc apoptosis detection kit, by Nacalai Tesque (1 mentions)
Facsaria 3, by BD (1 mentions)
2 protocols using tra 1 60 pe
1
Stem Cell Immunophenotyping Protocol
2
Comprehensive Flow Cytometry Analysis
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Apoptosis was evaluated using the Annexin V-FITC Apoptosis Detection Kit (Nacalai, 15342-54), which includes an Annexin V-FITC conjugate and propidium iodide (PI) according to the protocol provided by the manufacturer, and was further analyzed using flow cytometry. For the cell cycle analysis, Cell Cycle Assay Solution Deep Red (Dojindo, C548) was used; the solution provided with the kit was added to the cell suspension, followed by incubation according to the manufacturer’s protocol, and a flow cytometric analysis. For staining, CD105-APC (eBioscience, 17-1057-42), CD90-PE (eBioscience, 555596), CD73-PE (eBioscience, 550257), CD44-PE (eBioscience, 550989), CD34-PE (eBioscience, 560941), CD31-Alexa Fluor® 647 (BioLegend, 303112), and TRA-1-60-PE (Thermo Fisher, 12-8863-82) (1:50) was added to the cell suspension and incubated for 30 min before the flow cytometric analysis. BD FACSAria™ III was used to detect fluorescence. Results were plotted using FlowJo_v10.8.1 software.
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