The extracted DNA samples were quantified using a Quantifiler Trio Quantification kit (Thermo Fisher Scientific, Waltham, MA, USA) and an Applied Biosystems 7500 Real-Time Polymerase Chain Reaction (PCR) System (Thermo Fisher Scientific, Waltham, MA, USA). The cycling parameters were set and data analysis was performed in accordance with the recommendations of the manufacturer. The samples were amplified in triplicate. The DNA concentration was adjusted to 1.0 ng/μL in a final PCR mixture volume of 25 μL. The samples with higher DNA concentrations were diluted to 1 ng/μL.
The PCR product (1 μL) was added to 8.5 μL of highly deionized (Hi-Di) formamide (Applied Biosystems, Zug, Switzerland) and 0.5 μL of 600 LIZ (20–600 nucleotide range, Applied Biosystems) Size Standard (Applied Biosystems). STR amplification was performed using 1 ng of template DNA and an AmpFLSTR Identifiler PCR Amplification Kit (Thermo Fisher Scientific). Amplification was performed using an ABI Prism 310 Genetic Analyzer, and the data were analyzed with GeneMapper ID software v3.2.1 (Thermo Fisher Scientific, MA, USA).
The PCR product (1 μL) was added to 8.5 μL of highly deionized (Hi-Di) formamide (Applied Biosystems, Zug, Switzerland) and 0.5 μL of 600 LIZ (20–600 nucleotide range, Applied Biosystems) Size Standard (Applied Biosystems). STR amplification was performed using 1 ng of template DNA and an AmpFLSTR Identifiler PCR Amplification Kit (Thermo Fisher Scientific). Amplification was performed using an ABI Prism 310 Genetic Analyzer, and the data were analyzed with GeneMapper ID software v3.2.1 (Thermo Fisher Scientific, MA, USA).