The pyramidal probe (SN-AF01S-NT; spring constant: 0.02 N/m) was purchased from Seiko Instruments Inc. (Tokyo, Japan). Human embryonic kidney HEK293 cells were obtained from Health Science Research Resources Bank (Osaka, Japan). Cell anchoring molecule, BAM (SUNBRIGHT OE-020CS) (Fig. 1A ), was purchased from NOF Corporation (Tokyo, Japan). F-actin labeling kit was purchased from AAT Bioquest, Inc. (Sunnyvale, California, USA). Other reagents were purchased from Sigma-Aldrich (St. Louis, Missouri, USA), Wako Pure Chemical Industries Ltd. (Osaka, Japan), or Life Technologies Japan Ltd. (Tokyo, Japan).
Hek293 cells
Manufactured by Health Science Research Resources Bank
Sourced in Japan
HEK293 cells are immortalized human embryonic kidney cells commonly used in cell culture research. They serve as a model system for various experimental applications due to their ease of growth and transfection.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
F actin labeling kit, by AAT Bioquest (1 mentions)
Mscgm bulletkit, by Lonza (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Hela cells, by Health Science Research Resources Bank (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Hmscs, by Lonza (1 mentions)
3 protocols using hek293 cells
1
Cell Adhesion Protocol with BAM
2
Cell Culture Protocols of Various Cell Lines
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hMSCs at passage two (P = 2) were purchased from Lonza and cultured in MSCGM BulletKit (Lonza, Walkersville, MD, USA), a mesenchymal stem cell basal medium supplemented with mesenchymal cell growth supplement, l -glutamine, and gentamycin/amphotericin-B. HeLa cells (#JCRB9004), HEK293 cells at P = 42 (#JCRB9068), and MRC-5 cells at P = 23 (#JCRB9008) were obtained from the Health Science Research Resources Bank (Osaka, Japan) and were cultured in Eagle’s minimum essential medium supplemented with 10% fetal bovine serum (Merck, Darmstadt, Germany), 0.1 mM non-essential amino acids (Thermo Fisher Scientific, Waltham, MA, USA), 50 U/mL penicillin, and 50 μg/mL streptomycin (Thermo Fisher Scientific). Cells were maintained in the medium described above and passaged upon reaching 90% confluence until they were used for cell growth analysis.
3
DOTA Labeling of Anti-IGSF4 Antibody
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According to the protocol of one-pot three-component double click labeling of albumin with DOTA (preparation of 7b ), the labeling of anti-IGSF4 antibody with DOTA was carried out. The anti-IGSF4 mouse monoclonal antibody was raised against the extracellular domain of IGSF4 produced by HEK293 cells (Health Science Research Resources Bank, Osaka, Japan). Anti-IGSF4 antibody in PBS (3 × 10−5 M) was centrifuged with Amicon® with 14k rpm for 12 min and diluted with distilled water to give anti-IGSF4 antibody in water (2 × 10−5 M) prior to use. The labeling was performed with the antibody in water. The DOTA-attached anti-IGSF4 antibody 9a in water (2 × 10−5 M for each) were stocked for radiolabeling.
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