Plasma biochemistry analyses of glucose (GLU), total cholesterol (TC), triglycerides (TG), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total protein (TP), blood urea nitrogen (BUN), and creatinine (CRE) were performed using an autoanalyzer (JCA-BM2250, JEOL Ltd., Tokyo, Japan) according to the manufacturer’s protocol. The plasma non-esterified fatty acid (NEFA) concentration was measured using a Wako NEFA-C test commercial kit (Wako Pure Chemical Industries, Inc., Tokyo, Japan). Commercial kits were used for measurement of plasma MDA concentration (NWK-MDA01), SOD activity (NWK-SOD01) and GPx activity (NWK-GPX01, Northwest Life Science Specialties, LLC, Vancouver, Canada). Plasma LDH isozyme pattern was confirmed by a QuickGel LD Isoenzyme kit (Helena Laboratories, Texas, USA) with an Edbank III analysis software (Helena Laboratories, Texas, USA).
Jca bm2250
Manufactured by JEOL
Sourced in Japan
The JCA-BM2250 is a balanced-magnetron sputtering system designed for the deposition of thin films. It features a balanced-magnetron configuration for high-quality film deposition, with precise control over film thickness and uniformity. The system is suitable for a wide range of materials, including metals, alloys, and dielectric materials.
Automatically generated - may contain errors
Lab products found in correlation
Nefa c test, by Fujifilm (4 mentions)
Cat serum amyloid a elisa, by Life Diagnostics (2 mentions)
Edbank 3 analysis software, by Helena Laboratories (1 mentions)
Quickgel ld isoenzyme kit, by Helena Laboratories (1 mentions)
Labassay creatinine kit, by Fujifilm (1 mentions)
Pasteur pipette, by Thermo Fisher Scientific (1 mentions)
Nefa c test kit, by Fujifilm (1 mentions)
Glucose cii test wako, by Fujifilm (1 mentions)
18 protocols using jca bm2250
1
Comprehensive Plasma Biochemistry Analysis
2
Evaluating Blood Biomarkers in M-β-CyDs Treated Mice
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After 24 h of intravenous injection of M-β-CyDs (20 mg/kg) in mannitol solution (5%) to KB cells-xenografted mice, the blood was collected. The blood chemistry data of lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine (CRE), and blood urea nitrogen (BUN) in serum were analyzed using JCA-BM2250, a clinical chemistry analyzer (JEOL, Tokyo, Japan).
3
Metabolic Biomarkers in Plasma Analysis
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Pre-prandial blood was collected from jugular vein into heparinized plastic tubes. These samples were immediately centrifuged at 1,700 × g for 10 min at 4°C to obtain plasma, and the samples were stored at −80°C until use. Plasma GLU, TG, total cholesterol (TC) concentrations and plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT) activities were measured using autoanalyzer (JCA-BM2250, JEOL Ltd., Tokyo, Japan) with the manufacturer’s reagents at Monolis Inc., Tokyo, Japan. Plasma non-esterified fatty acid (NEFA) concentrations were measured with a commercial kit (NEFA-C test, Wako Pure Chemical Industries, Ltd., Tokyo, Japan). Plasma INS and ADN concentrations were measured with a commercial kit (Cat Insulin ELISA kit, Shibatagi Co., Ltd., Gunma, Japan; Mouse/Rat Adiponectin ELISA kit, Otsuka Pharmaceutical Co., Ltd., Tokyo, Japan), respectively. Statistical analysis for plasma parameters was performed by Mann–Whitney U-test (p < 0.01).
4
Plasma Biomarker Analysis Protocol
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Heparinized plasma was separated from the collected blood by centrifugation at 3000 ×g for 10 min. Plasma concentrations of alanine transaminase (ALT), aspartate aminotransferase (AST), total cholesterol (T-CHO), phospholipid (PL) and triglycerides (TG), and glucose in both the plasma and urine were measured using an automatic analyzer (JCA-BM 2250; JEOL Ltd., Tokyo, Japan). Plasma levels of insulin (Morinaga Institute of Biological Science Inc., Yokohama, Japan) and adiponectin (Otsuka Pharmaceutical Co. Ltd., Tokyo, Japan) were quantitated with ELISA kits.
5
Plasma and Serum Biomarker Analysis
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Plasma PT-INR and serum levels of Alb, alanine aminotransferase, ammonia (NH3), aspartate aminotransferase, and creatinine, as well as the platelet count and T-Bil, were determined by an autoanalyzer (JCA-BM2250; JEOL, Tokyo, Japan).
6
Serum Creatinine Quantification in Mice
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Serum mouse Cre concentrations were measured by using a Lab-AssayTM Creatinine Kit (Wako Pure Chemical Industries, Ltd., Osaka, Japan). BUN and Cre levels were analysed using the SIMENS Dimension RXL Max System (SIEMENS Co., Ltd.) or JCA-BM2250 (JEOL Ltd., Tokyo, Japan). The mice were selected for blood drawing in a randomised manner.
7
Plasma and Serum Biomarker Analysis
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Plasma PT and serum levels of ALT, AST, Cre, and T-Bil were determined using an autoanalyzer (JCA-BM2250; JEOL, Tokyo, Japan). Data were collected at ALF diagnosis.
8
Serum Biochemical Analysis in ICR Mice
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We carried out a clinical-biochemical test using serum collected from ICR females at 13 weeks of age. The mice consumed experimental diets for 7 weeks. Blood collection, separation of serum, and analysis was performed as previously described [20 (link)]. In brief, we collected 200 μl of blood from the retro-orbital sinus using a Pasteur pipette (Thermo Fisher Scientific Inc., Waltham, MA). The collected blood was transferred to a microtube containing Coagulant Wako solution (Wako Pure Chemical Industries Co, Osaka, Japan) and serum was separated by centrifuging twice (600–2500×g for 5–15 min). We measured total protein (TP), urea nitrogen (UN), albumin (ALB), total cholesterol (T-CHO), HDL-cholesterol (HDL-C), triglyceride (TG), and LDL-cholesterol (LD-C) in the serum with an automatic clinical-biochemistry analyzer (JCA-BM2250, JEOL, Ltd., Tokyo, Japan). Number of subjects: CD, 7; PR, 7; FA, 6.
9
Plasma Biomarkers Measurement
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Heparinized plasma was separated from the collected blood by centrifugation at 3000×g for 10 minutes. Plasma levels of glucose, as well as electrolytes including sodium, potassium and chlorine in heparinized plasma, were measured using an automatic analyzer (JCA‐BM 2250; JEOL, Tokyo, Japan). Plasma insulin was measured using a rat insulin ELISA kit (Morinaga Institute of Biological Science, Yokohama, Japan). The plasma 8‐iso‐prostaglandin F2α concentration was determined by a Plasma 8‐iso‐prostaglandin F2α ELISA kit (Cayman Chemical, Ann Arbor, MI, USA).
10
Serum Lipid Metabolite Profiling
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Serum lipid metabolites and enzyme activities were measured. Glucose (GLU), TG, total cholesterol (T-Cho), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein, blood urea nitrogen (BUN), and creatinine (CRE) were determined using a biochemical auto analyzer (JCA-BM2250, JEOL Ltd., Tokyo, Japan) with the manufacturer’s reagents. Oxidized LDL was determined using a Dog Oxidized low-density lipoprotein ELISA (Kameyama Biomedical Company, WA, USA). This ELISA kit uses a mouse monoclonal antibody for all of malondialdehyde (MDA)-LDL, Nepsilon (carboxymethyl) lysine (CML)-LDL, and detects advanced glycosylation end product (AGE)-LDL; it cannot distinguish between them. We constructed a calibration curve by plotting the average optical density for each calibrator on the horizontal (X) axis against the concentration on the vertical (Y) axis, and drawing the best-fit curve to generate a four parameter logistic (4-PL) curve-fit.
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