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Eclipse model e600 microscope

Manufactured by Nikon
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Sourced in Japan

The Nikon Eclipse Model E600 is a microscope designed for laboratory use. It features high-quality optics and advanced illumination systems to provide clear, high-resolution images. The microscope is capable of a wide range of magnification levels to accommodate various sample types and research requirements.

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Lab products found in correlation

Formalin, by Merck Group (4 mentions)

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Eclipse E600 (911 citations) Eclipse E600 microscope (471 citations) Eclipse microscope (191 citations) E600 microscope (119 citations) Nikon Eclipse E600 model (2 citations)

5 protocols using eclipse model e600 microscope

1

Lung Histological Assessment Post-Lavage

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Following lung lavage, whole lungs were excised and slowly inflated (15 cm H2O pressure) with 10% formalin (Sigma, St. Louis, MO) for 24 h to preserve pulmonary architecture as previously described [15 (link)]. Fixed lungs were processed, embedded in paraffin, and entire lung sections were cut (4-5 μm) and stained with hematoxylin and eosin (H&E). Each slide was entirely reviewed at scanning magnifications (2X, 4X, and 10X objectives; Nikon Eclipse Model E600 microscope, Nikon, Tokyo, Japan) and semi-quantitatively assessed for the degree and distribution of lung inflammation by a pathologist (W.W.W.), blinded to the treatment conditions, utilizing a previously published scoring system [15 (link)]. This scoring system evaluates the spectrum of inflammatory changes for: 1) alveolar compartment inflammation, 2) bronchiolar compartment inflammation, and 3) intrapulmonary lymphoid aggregates and is compared to a standard set of photomicrographs. The bronchiolar compartment consists of inflammatory infiltrates in both the peri-bronchiolar and per-vascular space. Each parameter was independently assigned a value from 0 to 3, with a higher score indicating greater inflammatory changes in the lung.
Poole J.A., Mikuls T.R., Duryee M.J., Warren K.J., Wyatt T.A., Nelson A.J., Romberger D.J., West W.W, & Thiele G.M. (2017). A role for B cells in organic dust induced lung inflammation. Respiratory Research, 18, 214.
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2

Lung Inflammation Scoring Protocol

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Following lung lavage, whole lungs were excised and slowly inflated (20 cm H2O pressure) with 10% formalin (Sigma) for 24 hours to preserve pulmonary architecture as previously described [6 (link)]. Fixed lungs were processed, embedded in paraffin, and entire lung sections were cut (4–5 μm) and stained with hematoxylin and eosin (H&E). Each slide was entirely reviewed at scanning magnifications (2X, 4X, and 10X objectives; Nikon Eclipse Model E600 microscope, Nikon, Tokyo, Japan) and semi-quantitatively assessed for the degree and distribution of lung inflammation by a pathologist (W.W.W.), blinded to the treatment conditions, utilizing a previously published scoring system [6 (link)]. This scoring system evaluates the spectrum of inflammatory changes for: 1) alveolar compartment inflammation, 2) bronchiolar compartment inflammation, and 3) intrapulmonary cellular aggregates. Each parameter was independently assigned a value from 0 to 3, and the greater the score, the greater the inflammatory changes in the lung.
Warren K.J., Wyatt T.A., Romberger D.J., Ailts I., West W.W., Nelson A.J., Nordgren T.M., Staab E., Heires A.J, & Poole J.A. (2017). Post-injury and resolution response to repetitive inhalation exposure to agricultural organic dust in mice. Safety (Basel, Switzerland), 3(1), 10.
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3

Lung Inflammation Scoring Protocol

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Following lung lavage, whole lungs were excised and slowly inflated (20 cm H2O pressure) with 10% formalin (Sigma) for 24 hours to preserve pulmonary architecture as previously described [6 (link)]. Fixed lungs were processed, embedded in paraffin, and entire lung sections were cut (4–5 μm) and stained with hematoxylin and eosin (H&E). Each slide was entirely reviewed at scanning magnifications (2X, 4X, and 10X objectives; Nikon Eclipse Model E600 microscope, Nikon, Tokyo, Japan) and semi-quantitatively assessed for the degree and distribution of lung inflammation by a pathologist (W.W.W.), blinded to the treatment conditions, utilizing a previously published scoring system [6 (link)]. This scoring system evaluates the spectrum of inflammatory changes for: 1) alveolar compartment inflammation, 2) bronchiolar compartment inflammation, and 3) intrapulmonary cellular aggregates. Each parameter was independently assigned a value from 0 to 3, and the greater the score, the greater the inflammatory changes in the lung.
Warren K.J., Wyatt T.A., Romberger D.J., Ailts I., West W.W., Nelson A.J., Nordgren T.M., Staab E., Heires A.J, & Poole J.A. (2017). Post-injury and resolution response to repetitive inhalation exposure to agricultural organic dust in mice. Safety (Basel, Switzerland), 3(1), 10.
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4

Standardized Lung Tissue Preservation and Histological Analysis

Cited 1 time
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After whole lung lavage, lungs were harvested, inflated with 1 ml 10% formalin, and hung under a pressure of 20 cmH2O for 1 day while submerged in 10% formalin for optimal preservation of lung parenchymal architecture as previously described (Poole, et al. 2014 (link)). Fixed lung tissues were standardly processed and embedded in paraffin, and sections (4–5 μM) cut and stained with hematoxylin and eosin. Each slide was reviewed in entirety, using (2x, 4x, and 10x objectives; Nikon Eclipse Model E600 microscope, Nikon, Tokyo, Japan) and semi-quantitatively assessed for the degree and distribution of lung inflammation utilizing a previously published scoring system by a lung pathologist blinded to the treatment conditions (Poole, et al. 2009 (link)). The scoring system is a Likert scale (0–3) with higher values representing increased histopathologic inflammation.
Poole J.A., Romberger D.J., Wyatt T.A., Staab E., VanDeGraaff J., Thiele G.M., Dusad A., Klassen L.W., Duryee M.J., Mikuls T.R., West W.W., Wang D, & Bailey K.L. (2015). Age Impacts Pulmonary Inflammation and Systemic Bone Response to Inhaled Organic Dust Exposure. Journal of toxicology and environmental health. Part A, 78(19), 1201-1216.
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5

Lung Inflammation Histological Assessment

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Following lung lavage, the whole lungs were excised and slowly inflated (and hung under 20 cm H2O pressure) with 10% formalin (Sigma) for 24 hr. Next, by routine histology processing, the fixed lung tissue was embedded in paraffin and entire lung sections cut (4–5 µm) and stained with hematoxylin and eosin (H&E). Each slide was entirely reviewed at scanning magnifications (2X, 4X, and 10X objectives; Nikon Eclipse Model E600 microscope, Nikon, Tokyo, Japan) and semi-quantitatively assessed for the degree and distribution of lung inflammation by a pathologist (W.W.W.), blinded to the treatment conditions, utilizing a previously published scoring system (Poole et al., 2009 ).
Poole J.A., Anderson L., Gleason A.M., West W.W., Romberger D.J, & Wyatt T.A. (2014). Pattern Recognition Scavenger Receptor A/CD204 Regulates Airway Inflammatory Homeostasis Following Organic Dust Extract Exposures. Journal of immunotoxicology, 12(1), 64-73.
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