Cay10502

Manufactured by Cayman Chemical

CAY10502 is a laboratory reagent manufactured by Cayman Chemical. It is a chemical compound that can be used in research applications.

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Lab products found in correlation

Lightcycler 480 sybr green 1 master mix, by Roche (1 mentions) α tubulin antibody, by Santa Cruz Biotechnology (1 mentions) Pge2 elisa kit, by Cayman Chemical (1 mentions) Hybond c nitrocellulose membrane, by GE Healthcare (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Rnalater, by Thermo Fisher Scientific (1 mentions) Quantitect reverse transcription kit, by Roche (1 mentions) Rneasy mini kit, by Roche (1 mentions) Leupeptin, by Roche (1 mentions) Pepstatin, by Roche (1 mentions) Fsl 1, by InvivoGen (1 mentions) Pam3csk4, by InvivoGen (1 mentions) Hybond, by Cytiva (1 mentions) Arachidonyl trifluoromethylketone, by Merck Group (1 mentions) Aacocf3, by Merck Group (1 mentions)

2 protocols using cay10502

Inflammatory Cytokine Signaling Assay

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PBS was from Oxoid. DNAse- and RNAse-free water was from VWR. Recombinant human TNF and IL-6 ELISA Duoset were from R&D systems. Quantitect primer assays for TLR1-7 and 18S were from Qiagen. QuantiTect Reverse Transcription kit, RNeasy minikit, Leupeptin, pepstatin and LightCycler 480 SYBR Green I Master mix were from Roche Molecular Biochemicals. RNAlater was from Life technologies. FSL-1 and Pam₃CSK₄ were from Invivogen. [³H]-arachidonic acid ([³H]-AA), and liquid scintillation cocktail Ultima Gold were from NEN Perkin Elmer. AVX002 and Inhibitor 28 were provided by Avexxin AS (Trondheim, Norway). Arachidonyl trifluoromethyl ketone (AACOCF3, ATK) was from Enzo Life Sciences. Varespladib (LY315920) was from Selleckchem. CAY10502, CAY10590 and PGE₂ ELISA kit were from Cayman Chemicals. Phospho-cPLA2 (Ser505) antibody was from Cell Signal Technology. α-tubulin antibody was from Santa Cruz Biotechnology. Polyclonal goat anti-mouse immunoglobulins horse radish peroxidase-conjugated secondary antibody was from Dako. Hybond-C nitrocellulose membranes were from GE healthcare. NuPAGE gel system (10% Bis-Tris gel) was from Invitrogen. Hybond-C nitrocellulose membranes were from GE healthcare. SuperSignal West Femto Maximum Sensivity substrate was from Thermo Scientific. All other reagents were from Sigma-Aldrich.

Sommerfelt R.M., Feuerherm A.J., Skuland T, & Johansen B. (2015). Cytosolic Phospholipase A2 Modulates TLR2 Signaling in Synoviocytes. PLoS ONE, 10(4), e0119088.

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Quantifying Cell Injury and Survival in RPE Cells

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Lactate dehydrogenase (LDH) release was used to quantitatively access cell injury in cells exposed to SI. Inhibitors of PLA₂ were used to identify their influence on RPE cell survival. The iPLA₂-VIA-specific inhibitors included bromoenol lactone (BEL; BioNordika, Herlev, Denmark, Cayman Chemicals, Product number 13179) and 1, 1, 1, 2, 2- pentafluoro- 7- phenyl- 3- heptanone (FKGK; Cayman). Additionally, the cPLA₂-IVA inhibitor CAY 10502 (Cayman) as well as the combined cPLA₂-IVA and iPLA₂-VIA inhibitor arachidonyl trifluoromethylketone (AACOCF3; Sigma) were used. LDH-release was assayed using a MWG Discovery HT-R spectrophotometer. In addition, cell viability was determined by the colorimetric assay 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) according to the manufacturer's instructions.

Kolko M., Vohra R., Westlund van der Burght B., Poulsen K, & Nissen M.H. (2014). Calcium-independent phospholipase A2, group VIA, is critical for RPE cell survival. Molecular Vision, 20, 511-521.

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