Potassium hexacyanoferrate 3

Acetic acid (99.5%), aluminum chloride hexahydrate, copper(II) chloride dihydrate, disodium hydrogen phosphate dihydrate, 96% ethanol, 36% hydrochloric acid, iron(III) chloride hexahydrate, methanol, phosphoric acid, potassium persulfate, potassium dihydrogen phosphate, potassium hexacyanoferrate(III), sodium acetate anhydrous, sodium carbonate anhydrous, sodium hydroxide, sodium nitrite, and trichloroAcetic acid were purchased from Chempur, Poland. Neocuproine was delivered by J&K Scientific, Germany. Folin–Ciocalteu reagent, acetonitrile, iron(II) sulfate heptahydrate, gallic acid were supplied by Merck, Germany, whereas rutin trihydrate by Roth, Germany. ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-tris(2-pyridyl)-s-triazine), Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), caffeine were purchased from Sigma-Aldrich, USA. All the chemicals were of analytical grade.

Phosphate-buffered saline (PBS), 4-aminobenzoic acid, N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), and PCR primers were purchased from Sigma-Aldrich (Germany). Potassium hexacyanoferrate (III), hexaammineruthenium (III), sodium nitrite, hydrochloric acid, and dipotassium phosphate monopotassium phosphate were purchased from ChemPur (Poland). An ExtractMe DNA Bacteria kit was purchased from Blirt (Poland), and a SensiFAST SYBR No-ROX kit was purchased from Bioline (UK). Phosphate buffer with different pH (5.68, 7.01, 7.99) was prepared by mixing the K₂HPO₄ and KH₂PO₄ in different ratios. All reagents were used without further purification. Aqueous solutions were prepared with the usage of double-distilled sterile water (ddH₂O).
All biomaterials utilised in this manuscript are accurately described elsewhere [30 (link)]. Herein, we present them briefly. The monoclonal mouse antibodies were produced by the Nanobioengineering Laboratory at the Polish Center for Technology Development Ltd. through procedures described elsewhere [31 ], with slight changes. Recombinant protein D was isolated at the Institute of Biotechnology and Molecular Medicine following the standard procedures. The efficiency and quality of antibody production were verified with the ELISA test (data are not shown).