Hydrochloroauric acid trihydrate (HAuCl4∙3H2O), caffeic acid, 4-nitrophenol, 4-aminophenol, ninhydrin, acetic acid and NaBH4 were purchased from Sigma-Aldrich (St. Louis, MO, USA). DMSO-d6 was obtained from Armar Chemicals (Dottingen, Switzerland). All other reagents were of analytical grade. TLC analyses were performed using Merck pre-coated TLC plates (silica gel 60 GF254, 0.25 mm, Germany). Syringe filtration was conducted using Minisart RC syringe filters (hydrophilic, 0.2 μm, Sartorius Stedim Biotech GmbH, Goettingen, Germany). All solutions were prepared in deionized water.
Minisart rc syringe filters
Manufactured by Sartorius
Sourced in Germany
Minisart RC syringe filters are a type of lab equipment designed for rapid filtration of small sample volumes. They feature a hydrophilic regenerated cellulose membrane that effectively removes particulates and bacteria from samples. These syringe filters are easy to use and available in a range of pore sizes to suit various filtration needs.
Automatically generated - may contain errors
Lab products found in correlation
Caffeic acid, by Merck Group (1 mentions)
Nabh4, by Merck Group (1 mentions)
Ninhydrin, by Merck Group (1 mentions)
Hydrochloroauric acid trihydrate, by Merck Group (1 mentions)
4 nitrophenol, by Merck Group (1 mentions)
4 aminophenol, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
2 protocols using minisart rc syringe filters
1
Chemical Synthesis Protocol Optimization
2
Extraction of Crude Protein from Germinated Chestnuts
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The crude protein solution was prepared according to the schematic diagram shown in Fig. S1 . The germinated chestnuts (5.0 kg) were ground using a blender in 7.0 L of 100 mM Tris-HCl (pH 7.0). Four liters of n-hexane/diethyl ether (1:1, v/v) were added to the mixture, which was stirred at 4 °C for 1 h. Then, the mixture was centrifuged at 3000×g for 15 min at 4 °C to separate the lipid and aqueous layers. A separatory funnel was used to collect the aqueous phase, and the solution was centrifuged at 10,000×g for 30 min at 4 °C. The precipitate was then removed, and the supernatant was filtered through 0.45-μm Minisart RC syringe filters (Sartorius, Goettingen, Germany). The crude protein sample was collected into a bottle and stored at 4 °C for subsequent experiments.
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