Animals were dark-adapted for more than 1 h before experiments. Under a dim red light, a goldfish was double-pithed and eyes were enucleated. The following procedure was performed under a stereomicroscope equipped with infrared (IR) image converter (C5100, Hamamatsu photonics) and IR illuminator (HVL-IRM, Sony). After the cornea and lens were ablated, the eye cup was treated with a mixture of hyaluronidase and collagenase (4 mg/mL each, Sigma-Aldrich Corp.) for a few min. A small cut was made at the dorsal part of the eye cup as a landmark and thus the ventral retina isolated from the pigment epithelium was properly oriented and positioned on the multi-electrode array.
C5100
Manufactured by Hamamatsu Photonics
The C5100 is a highly sensitive digital CCD camera system designed for low-light imaging applications. It features a back-illuminated CCD sensor with high quantum efficiency and low noise characteristics. The C5100 provides high-resolution image capture and can be used in a variety of scientific and industrial applications.
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Lab products found in correlation
2 protocols using c5100
1
Goldfish Retina Extraction Protocol
2
Goldfish Retina Isolation for Electrophysiology
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Goldfish were dark-adapted for more than 1 h before experiments. Under a dim red light, a goldfish was double-pithed, and eyes were enucleated. The following procedure was performed under a stereomicroscope equipped with infrared (IR) image converter (C5100, Hamamatsu photonics) and IR illuminator (HVL-IRM, Sony). After the cornea and lens were ablated, the eye cup was treated with a mixture of hyaluronidase and collagenase (4 mg/mL each, Sigma-Aldrich Corp.) for a few min. A small cut was made at the dorsal part of the eye cup as a landmark and thus the ventral retina isolated from the pigment epithelium was properly oriented and positioned on the multi-electrode array or in the recording chamber for whole-cell recordings.
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