Potassium chloride p3911

Medium molecular weight chitosan with a deacetylation degree of 75–85% (448877) and high molecular weight chitosan with a >75% deacetylation degree (419419) were purchased from Sigma-Aldrich (Burlington, MA, USA)and used as received. Ascorbic acid (AC05150) and acetic acid (AC0344) were purchased from Scharlau (Spain), and Tween^® 20 (Polysorbate) (97062-332) was purchased from VWR (Radnor, PA, USA) and used as received. Grape seed essential oil was purchased from Herbavit (Bucharest, Romania), and sea buckthorn essential oil was purchased from Hofigal (Bucharest, Romania) and used as received. Folin–Ciocalteu reagent (F9252), xylene (214736), sodium bicarbonate (S6014), sodium acetate (S8750), 2,2-diphenyl-1-picrylhydrazyl (DPPH) (247642), and potassium chloride (P3911) were purchased from Merck (Darmstadt, Germany).

All standard solutions used for calibration purposes were prepared dissolving the chemical compound in bi-distilled water at the higher concentration and by following dilutions. All chemical standards were purchased at Sigma Aldrich (Merck KGaA, Darmstadt, Germany): potassium chloride (P3911), sodium chloride (S7653), sodium bicarbonate (S5761), calcium chloride (C5670), magnesium chloride (M8266), Sodium hydrosulfide hydrate (161527), dopamine hydrochloride (H8502). All these samples were prepared and measured in glass test-tubes.
The BIONOTE was initially challenged against these CSF constituents individually as described in [13 (link)]. Seven calibration curves were prepared using the chemical standard compounds reported in Table 1.