D-(-)-2-Amino-5-phosphonopentanoic acid (AP-5), Nomega-Nitro-L-arginine methyl ester hydrochloride (L-NAME), (S)-1-(2-Amino-2-carboxyethyl)-3-(2 -carboxy-thiophene-3-yl-methyl)-5-methylpyrimidine-2,4-dione (UBP 310), picrotoxin, and Ro 25-6981 were purchased from HelloBio (Princeton, NJ, USA). Nimodipine, ODQ, BAPTA, Rp-8-pCPT-cGMP, and picrotoxin were bought from Sigma-Aldrich (St Louis, MO, USA). Drugs were prepared as stock solutions for frozen aliquots at −20 °C. All drugs were diluted from the stock solution to the final concentration in the ACSF before being applied to brain slices.
Ro 25 6981
Manufactured by Hello Bio
Sourced in United States
Ro 25-6981 is a selective, potent, and non-competitive NMDA receptor antagonist. It acts by binding to the NR2B subunit of the NMDA receptor.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using ro 25 6981
1
Pharmacological Modulation of Neuronal Activity
2
Pharmacological Modulation of Olfactory Processing
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For behavior, Ro25–6981 (Ro25; 5mg/kg, saline) and methyl-piperidino-pyrazole (MPP; 0.6 mg/kg, 2% DMSO/saline) were injected intraperitoneally 30 and 60 min before exposure to odors, respectively. For electrophysiology, compounds were introduced to the slice bath via a syringe pump (6ml/hr) into the aCSF infusion line for final bath concentrations: MK801 (30μM; Tocris, 0924), APV (100μM; Hello Bio, HB0225), DNQX (20μM; Hello Bio, HB0261), picrotoxin (30μM; Sigma-Aldrich, P1675) in water. MPP (3μM; Tocris, 1991) and Ro25–6981 (3μM; Hello Bio, HB0554) were dissolved with DMSO (≤0.01%).
3
Pharmacological Agents in Neuroscience
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The chemical and drugs used in this study were as follows: AP-5, CNQX and Ro 25-6981 purchased from HelloBio (Princeton, NJ, USA), PPDA, UBP145 and PEAQX were purchased from Tocris Cookson (Bristol, UK), PTX was bought from Sigma-Aldrich (St Louis, MO, USA). Drugs were prepared as stock solutions for frozen aliquots at -20℃. All drugs were diluted from the stock solution to the final desired concentration in the ACSF before being applied to the perfusion solution.
4
Methamphetamine Reinstatement and NMDAR Antagonist
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(+) Methamphetamine HCL (METH; Sigma-Aldrich, St. Louis, MO) was dissolved in 0.9% saline and self-administered i.v. at a unit dose of 0.1 mg/kg/infusion (calculated as the weight of the salt). Thirty min prior to the reinstatement session, rats were injected with METH (1 mg/kg i.p.). Ro 25-6981, an antagonist selective for the GluN2B subunit of the NMDAR, was obtained from Hello Bio, Inc (Princeton, NJ) and dissolved in 1 part dimethyl sulfoxide (DMSO) and 2 parts 0.9% saline. Ro 25-6981 or vehicle solution was injected i.p. immediately after each of the four short extinction sessions at a dose of 6 mg/kg (1 ml/kg volume).
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