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Fluorescent tunel staining kit

Manufactured by Roche
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The Fluorescent TUNEL staining kit is a laboratory product that enables the detection of DNA fragmentation, a hallmark of apoptosis or programmed cell death. The kit utilizes the TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) technique to label the free 3'-hydroxyl termini of fragmented DNA with fluorescent dUTP. This allows for the visualization and quantification of apoptotic cells using fluorescence microscopy or flow cytometry.

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Lab products found in correlation

Prism 7, by GraphPad (2 mentions)

Spelling variants of this product

TUNEL kit (509 citations) TUNEL staining kit (135 citations) TUNEL staining (96 citations) TUNEL fluorescent kit (5 citations) Fluorescent TUNEL staining (2 citations)

2 protocols using fluorescent tunel staining kit

1

Apoptosis Quantification in Duck Embryos

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10 μm tissue sections of duck embryos 24 h after treatment with SU5402, SB431542, or DMSO soaked beads were processed using a fluorescent TUNEL staining kit (Roche, Basel, Switzerland). As a positive control, DNase was added to a subset of DMSO-treated tissue sections. The percentage of cell death was quantified using 3D microscopy processing software Imaris (Bitplane, Belfast, United Kingdom). Image intensity was rendered in 3D and Hoescht (Sigma-Aldrich, St. Louis, MO) and TUNEL-stained nuclei within 100 μm of the implanted bead were counted using software-enabled volumetric criteria (surface detail = 5 μm, background subtraction = 12 μm, seed point diameter = 30 μm). Statistical significance was determined by ordinary one-way ANOVA (Prism 7, GraphPad Software, Inc., La Jolla, CA).
Woronowicz K.C., Gline S.E., Herfat S.T., Fields A.J, & Schneider R.A. (2018). FGF and TGFβ signaling link form and function during jaw development and evolution. Developmental biology, 444(Suppl 1), S219-S236.
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2

Apoptosis Quantification in Duck Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols
10 μm tissue sections of duck embryos 24 h after treatment with SU5402, SB431542, or DMSO soaked beads were processed using a fluorescent TUNEL staining kit (Roche, Basel, Switzerland). As a positive control, DNase was added to a subset of DMSO-treated tissue sections. The percentage of cell death was quantified using 3D microscopy processing software Imaris (Bitplane, Belfast, United Kingdom). Image intensity was rendered in 3D and Hoescht (Sigma-Aldrich, St. Louis, MO) and TUNEL-stained nuclei within 100 μm of the implanted bead were counted using software-enabled volumetric criteria (surface detail = 5 μm, background subtraction = 12 μm, seed point diameter = 30 μm). Statistical significance was determined by ordinary one-way ANOVA (Prism 7, GraphPad Software, Inc., La Jolla, CA).
Woronowicz K.C., Gline S.E., Herfat S.T., Fields A.J, & Schneider R.A. (2018). FGF and TGFβ signaling link form and function during jaw development and evolution. Developmental biology, 444(Suppl 1), S219-S236.
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