Pe anti human pd l1

AURKA inhibitors VX‐680 and MLN8237 and bromodomain and extra‐terminal (BET) bromodomain inhibitor JQ1 were obtained from Selleck Chemicals (Houston, TX, USA) and dissolved in dimethyl sulfoxide (DMSO). MDA‐MB‐231 cells were treated with 0.1, 0.2, and 0.4 μmol/L VX‐680 and 0.1, 0.2, 0.4, and 0.8 μmol/L MLN8237 for 24 h. MDA‐MB‐231 cells with NLS‐AURKA were treated with 1 and 5 μmol/L JQ1 for 48 h for Western blotting detection. Antibodies against AURKA and phospho‐AURKA (T288) were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH), goat anti‐mouse IgG‐horse radish peroxidase (HRP), and goat anti‐rabbit IgG‐HRP were obtained from Cell Signaling (Danvers, MA, USA). c‐Myc was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). PD‐L1 was obtained from Abcam (Cambridge, MA, USA). TruStain FcX™ anti‐mouse CD16/32, PerCP anti‐mouse CD45, FITC anti‐mouse CD3, PE anti‐mouse CD4, APC anti‐mouse CD8a, and PE anti‐mouse CD69 used in flow cytometric analysis were purchased from Biolegend (San Diego, CA, USA). PE anti‐human PD‐L1 and its isotype control PE anti‐human IgGk1 were obtained from BD Pharmingen (San Diego, CA, USA).