Mammary tumors, LNs, and the main mouse organs were harvested, fixed, dehydrated, embedded in paraffin and sectioned in 5-μm slides. Then, samples were deparaffined, hydrated, and autoclaved for 11 min at 126 °C in Target Retrieval Solution (Dako). Endogenous peroxidase activity was quenched (3% H2O2 in PBS), and sections were blocked. For lymphatic vasculature detection, a primary polyclonal LYVE-1 antibody was used (1/100, R&D Systems) and samples were revealed with the Phenol Red chromogen kit (Dako). To detect MDA-MB-231/Luc+ cells, a mouse monoclonal anti-human-ki-67 antibody (1/100, clone MIB-1, M7240, Dako) was applied and the signal was developed by treatment with 3,3’-diaminobenzidine (DAB, Dako). Finally, samples were counterstained with hematoxylin/eosin, washed, dehydrated, mounted, and visualized.
Lyve 1 antibody
Manufactured by R&D Systems
The LYVE-1 antibody is a laboratory research tool used to detect and analyze the LYVE-1 protein. LYVE-1 is a lymphatic endothelial hyaluronan receptor that plays a role in lymphatic vessel development and function. The antibody can be used in various immunoassay techniques to identify and study the LYVE-1 protein in biological samples.
Automatically generated - may contain errors
2 protocols using lyve 1 antibody
1
Immunohistochemical Analysis of Mammary Tumor
2
Imaging Liver-Infiltrating T Cells and Antigen Presentation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Formalin‐fixed paraffin liver sections were stained with haematoxylin and eosin to visualize biliary inflammation. For evaluation of the liver‐infiltrating congenic OT‐1 cells (CD45.1+) and their interaction with cholangiocytes (CK19+), cryosections of liver tissue were stained with PE‐labelled antibody to CD45.1 (BioLegend) and primary antibody to CK19 (DSHB Hybridoma Product TROMA‐III) together with AF488‐labelled secondary antibody. Nuclei were stained with Hoechst 33342. To visualize MHC I‐restricted SIINFEKL presentation by cholangiocytes or cross‐presentation by LSECs, SIINFEKL‐H2Kb (eBioscience) was costained with CK19 antibody (DSHB Hybridoma Product TROMA‐III) or LYVE‐1 antibody (R&D systems). As indicated in the figure legends, AF488‐ or AF546‐labelled secondary antibodies have been used (Invitrogen).
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