Rabbit polyclonal anti gapdh

The source and catalog number of the antibodies we used were as follows: rabbit IgG (Santa Cruz, SC2027); rabbit polyclonal anti-AGO1 (MBL, RN028PW); rat monoclonal anti-AGO2 (Sigma, SAB4200085); mouse monoclonal anti-AGO3 (Active Motif, 39787); mouse monoclonal anti-AGO4 (Merck Millipore, 05-967); mouse monoclonal anti-Flag (Sigma, A8592); rabbit polyclonal anti-GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (Bioworld, AP0063); rabbit polyclonal anti-HIWI (custom-made by GL Biochem); rabbit polyclonal anti-HIWI3 (custom-made by GL Biochem); rabbit polyclonal anti-HILI (custom-made by GL Biochem).

The antibodies used in this study are as follows: mouse monoclonal anti-p62 (Abcam, ab56416, 1:100,000), mouse monoclonal anti-FK2 specific to Ub-conjugated proteins (Millipore, 04-263, 1:1,000), rabbit polyclonal anti-LC3 (Sigma, L7543, 1: 10,000 for immunoblotting; 1:1,000 for immunofluorescence staining), anti-casapse-8 (Santa Cruz, sc-56070 1:2000 for immunoblotting, 1:200 for immunofluorescence staining), rabbit polyclonal anti-GAPDH (Santa Cruz, sc-25778, 1:2,000), rabbit polyclonal anti-GAPDH (BioWorld, AP0063, 1:20,000). The following secondary antibodies were used: alexa fluor 488 goat anti-rabbit IgG (Invitrogen, A11029, 1:200), texas red goat anti-mouse IgG (Invitrogen, T6390, 1:500), anti-rabbit IgG-HRP (Cell Signaling, 7074, 1:10,000), and anti-mouse IgG-HRP (Cell Signaling Technology, 7076, 1:10,000). Other reagents used in this study were bafilomycin A1 (Sigma); high capacity streptavidin agarose resin (Thermo Fisher Scientific).