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Sirt1 fluorometric assay kit

Manufactured by Merck Group
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Sourced in United States

The Sirt1 Fluorometric Assay kit is a laboratory tool designed to measure the activity of the Sirt1 enzyme. It utilizes a fluorogenic substrate to quantify Sirt1 deacetylase activity in a simple, sensitive, and high-throughput format.

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Lab products found in correlation

Nuclear cytosol fractionation kit, by Abcam (2 mentions)

Close spelling variants of this product

Fluorometric Assay Kit Fluorometric kits SIRT1

4 protocols using sirt1 fluorometric assay kit

1

Measuring Sirt1 Deacetylase Activity

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To measure cellular Sirt1 deacetylase activity, nuclear proteins were extracted from SH-SY5Y cells using a Nuclear/Cytosol Fractionation kit (BioVision, Milpitas, CA, USA). Sirt1 deacetylase activity was quantified following the protocols of the Sirt1 Fluorometric Assay kit (Sigma-Aldrich). Fluorescence intensities were measured with a microplate fluorometer (excitation wavelength = 360 nm, emission wavelength = 450 nm). The fluorescence intensities of Sirt1 deacetylase activity were normalized to the protein levels measured in the cell samples.
Lee J.H., Moon J.H., Kim S.W., Jeong J.K., Nazim U.M., Lee Y.J., Seol J.W, & Park S.Y. (2015). EGCG-mediated autophagy flux has a neuroprotection effect via a class III histone deacetylase in primary neuron cells. Oncotarget, 6(12), 9701-9717.
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2

SIRT1 Activity Quantification Protocol

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SIRT1 activity was quantified with a SIRT1 Fluorometric Assay Kit (Sigma, St. Louis, MO, USA) according to the manufacturer's protocol. Fluorescence intensities were measured with a microplate fluorometer (excitation wavelength=360 nm, emission wavelength=450 nm). Experimental values are represented as a percentage of control.
Chai R., Fu H., Zheng Z., Liu T., Ji S, & Li G. (2017). Resveratrol inhibits proliferation and migration through SIRT1 mediated post-translational modification of PI3K/AKT signaling in hepatocellular carcinoma cells. Molecular Medicine Reports, 16(6), 8037-8044.
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3

SIRT1 Activity Quantification

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SIRT1 activity was measured by SIRT1 Fluorometric Assay Kit (Sigma‐Aldrich) according to the instruction provided with the kit.
Du K., Fang X, & Li Z. (2021). Ferulic acid suppresses interleukin‐1β‐induced degeneration of chondrocytes isolated from patients with osteoarthritis through the SIRT1/AMPK/PGC‐1α signaling pathway. Immunity, Inflammation and Disease, 9(3), 710-720.
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4

Quantifying Sirt1 Deacetylase Activity

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To measure cellular Sirt1 deacetylase activity, nuclear proteins were extracted from HaCaT keratinocytes using a Nuclear/Cytosol Fractionation kit (BioVision, Milpitas, CA, USA). Sirt1 deacetylase activity was quantified following the protocols of the Sirt1 Fluorometric Assay kit (Sigma-Aldrich). Fluorescence intensities were measured with a microplate fluorometer (excitation wavelength = 360 nm, emission wavelength = 450 nm). The fluorescence intensities of Sirt1 deacetylase activity were normalized to the protein levels measured in the cell samples.
Lee J.H., Moon J.H., Nazim U.M., Lee Y.J., Seol J.W., Eo S.K., Lee J.H, & Park S.Y. (2016). Melatonin protects skin keratinocyte from hydrogen peroxide-mediated cell death via the SIRT1 pathway. Oncotarget, 7(11), 12075-12088.
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