Cell culture and DNA transfection reagent, lipofectamine were purchased from Invitrogen Inc. (Carlsbad, CA). Normal primary human bone marrow mononuclear cells were purchased from ATCC (Manassas, VA) and peripheral blood monocytes (PBMC) were obtained from Stem Cell Technologies Inc., (Vancouver, BC). RANKL and M-CSF were obtained from R&D systems Inc. (Minneapolis, MN). Anti-c-Fos, anti-SIRPβ1 and peroxidase-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-DAP12, Syk and p-Syk antibodies were obtained from the Cell Signaling (Danvers, MA). SuperSignal enhanced chemiluminescence (ECL) reagent was purchased from the Amersham Bioscience (Piscataway, NJ), and nitrocellulose membranes were from Millipore (Bedford, MA).
Supersignal enhanced chemiluminescence ecl reagent
Manufactured by Cytiva
SuperSignal enhanced chemiluminescence (ECL) reagent is a luminol-based solution designed for the detection of proteins on Western blots. It generates a chemiluminescent signal when in contact with peroxidase-conjugated antibodies, enabling the visualization of target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (2 mentions)
Nitrocellulose membrane, by Merck Group (1 mentions)
Lipofectamine, by Thermo Fisher Scientific (1 mentions)
Anti c fos, by Santa Cruz Biotechnology (1 mentions)
Anti dap12, by Cell Signaling Technology (1 mentions)
M csf, by R&D Systems (1 mentions)
Rankl, by R&D Systems (1 mentions)
Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Draq5, by Thermo Fisher Scientific (1 mentions)
3 3 diaminobenzidine (dab), by Vector Laboratories (1 mentions)
Alexa 488 conjugated anti rabbit igg secondary antibody, by Thermo Fisher Scientific (1 mentions)
Hrp labeled secondary antibody, by Vector Laboratories (1 mentions)
Anti lc3 2, by Cell Signaling Technology (1 mentions)
2 protocols using supersignal enhanced chemiluminescence ecl reagent
1
Osteoclast Differentiation Regulation
2
Autophagy-Related Protein Detection
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