Erb 041

Cell cultures were treated with 100 nmol/L UCN1, 40 nmol/L of PPT, a selective agonist for ERA or 50 nmol/L of ERB‐041, a selective agonist for ERB (all from Tocris Bioscience), and incubated for 24 h before harvesting.

The human ovarian cancer cell line OVCAR-3 was obtained from American Type Culture Collection (ATCC #HTB-161, Manassas, USA), and OAW-42 ovarian cancer cells were obtained from Sigma Aldrich (#85073102, St. Louis, USA). The cells were maintained in phenol red-free DMEM culture medium that was obtained from Invitrogen (Karlsruhe, Germany) containing FCS that was purchased from PAA (Pasching, Austria). RNeasy Mini Kit was obtained from Qiagen (Hilden, Germany). Transfectin reagent was obtained from BioRad (Hercules, USA). OptiMEM medium were purchased at Invitrogen (Karlsruhe, Germany). ESR2 and control siRNAs were from Ambion (Life Technologies, USA). Serum Replacement 2 (SR2) cell culture supplement and 17-β estradiol were from Sigma-Aldrich (Deisenhofen, Germany). ERβ agonists ERB-041 and WAY-200070 were from Tocris (Bristol, UK). 5α-androstane-3β, 17β-diol (3β-Adiol) was from Sigma (Deisenhofen, Germany) and Liquiritigenin from Extrasynthese (Lyon, France).

Phenol red-free DMEM culture medium was obtained from Invitrogen (Karlsruhe, Germany), FCS was purchased from PAA (Pasching, Austria). MDA-MB-231 and HS578T breast cancer cells were obtained from American Type Culture Collection (Manassas, USA). RNeasy Mini Kit was obtained from Qiagen (Hilden, Germany). Transfectin reagent was obtained from BioRad (Hercules, USA). OptiMEM medium were purchased at Invitrogen (Karlsruhe, Germany). ESR2 and control siRNAs were from Ambion (Life Technologies, USA). Serum Replacement 2 (SR2) cell culture supplement was from Sigma-Aldrich (Deisenhofen, Germany). ERβ agonists ERB-041 and WAY-200070 were from Tocris (Bristol, UK). 5α-androstane-3β, 17β-diol (3β-Adiol) was from Sigma (Deisenhofen, Germany) and Liquiritigenin from Extrasynthese (Lyon, France).