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Streptavidin phycoerythrin solution

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Streptavidin phycoerythrin solution is a fluorescent labeling reagent used in various bioanalytical applications. It consists of the protein streptavidin conjugated to the fluorescent dye phycoerythrin. This solution can be used to detect and quantify biotinylated biomolecules, such as proteins, nucleic acids, and cells, through specific binding interactions.

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Lab products found in correlation

Genechip scanner 3000, by Thermo Fisher Scientific (6 mentions) Biotinylated anti streptavidin, by Vector Laboratories (6 mentions) Nanodrop, by Thermo Fisher Scientific (5 mentions) Bioanalyzer, by Agilent Technologies (5 mentions) Fluidics station, by Thermo Fisher Scientific (3 mentions) Agcc software, by Thermo Fisher Scientific (2 mentions) Cytoscan hd genechip, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Streptavidin-phycoerythrin (17 citations) Streptavidin solution (2 citations)

6 protocols using streptavidin phycoerythrin solution

1

Rat Genome 2.0 ST GeneChip Analysis

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RNA samples were prepared from three independent SCG axons and parental soma. Samples were prepared according to Affymetrix protocols (Affymetrix). RNA quality and quantity was monitored using the Bioanalyzer (Agilent) and NanoDrop (ThermoFisher Scientific) respectively. Per RNA labeling, 200 nanograms of total RNA was used in conjunction with the Affymetrix recommended protocol for the GeneChip 2.0 ST chips. The hybridization cocktail containing the fragmented and labeled cDNAs was hybridized to the Affymetrix Rat Genome 2.0 ST GeneChip. The chips were washed and stained by the Affymetrix Fluidics Station using the standard format and protocols as described by Affymetrix. The probe arrays were stained with streptavidin phycoerythrin solution (Molecular Probes, Carlsbad, CA) and enhanced by using an antibody solution containing 0.5 mg/mL of biotinylated anti-streptavidin (Vector Laboratories, Burlingame, CA). An Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays. Gene expression intensities were calculated using Affymetrix AGCC software. Partek Genomic Suite was used to normalize for Robust Multi-array Average (Robust Multichip Analysis), summarize the probes, log2 transform the data and conduct ANOVA analysis.
Aschrafi A., Kar A.N., Gale J., Elkahloun A.G., Vargas J.N., Sales N., Wilson G., Tompkins M., Gioio A.E, & Kaplan B.B. (2016). A Heterogeneous Population of Nuclear-Encoded Mitochondrial mRNAs Is Present in the Axons of Primary Sympathetic Neurons. Mitochondrion, 30, 18-23.
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2

Whole-Genome Microarray Analysis Protocol

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Samples were prepared according to Affymetrix protocols (Affymetrix, Inc). DNA quality and quantity was ensured using Bioanalyzer (Agilent, Inc) and NanoDrop (Thermo Scientific, Inc) respectively. Per DNA labeling, 200 nanograms of genomic DNA were used in conjunction with the Affymetrix recommended protocol for CytoScan HD array kit and reagents (catalog# 901835). The hybridization cocktail containing the fragmented and labeled DNAs was hybridized to The Affymetrix CytoScan HD GeneChip. The chips were washed and stained by the Affymetrix Fluidics Station using the standard format and protocols as described by Affymetrix. The probe arrays were stained with streptavidin phycoerythrin solution (Molecular Probes, Carlsbad, CA) and enhanced by using an antibody solution containing 0.5 mg/mL of biotinylated anti-streptavidin (Vector Laboratories, Burlingame, CA). An Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays. The .Cel files were generated from the scanned images using Affymetrix AGCC software and the .cyhd.cychp files were generated by the Chromosome Analysis Suite (ChAS) Version 2.1 software.
All the analyses were done with ChAS default parameters for LOH and Copy Number State (CNS). A description of the samples used for this analysis is listed in Supplementary Table 5.
Arafeh R., Qutob N., Emmanuel R., Keren-Paz A., Madore J., Elkahloun A., Wilmott J.S., Gartner J.J., Di Pizio A., Winograd-Katz S., Sindiri S., Rotkopf R., Dutton-Regester K., Johansson P., Pritchard A., Waddell N., Hill V.K., Lin J.C., Hevroni Y., Rosenberg S.A., Khan J., Ben-Dor S., Niv M.Y., Ulitsky I., Mann G.J., Scolyer R.A., Hayward N.K, & Samuels Y. (2015). Recurrent inactivating RASA2 mutations in melanoma. Nature genetics, 47(12), 1408-1410.
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3

Affymetrix Microarray Gene Expression Analysis

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Per RNA labeling, 500 ηg of total RNA was used in conjunction with the Illumina® TotalPrep RNA Amplification Protocol (Ambion/Applied Biosystems, Cat#AMIL1791). The hybridization cocktail containing the fragmented and labeled cRNAs was hybridized to the Affymetrix Human Genome U133 2.0 Gene Chip. The chips were washed and stained by the Affymetrix Fluidics Station using the standard format and protocols as described by Affymetrix. The probe arrays were stained with streptavidin phycoerythrin solution (Molecular Probes, Carlsbad, CA) and enhanced by using an antibody solution containing 0.5 mg/mL of biotinylated anti-streptavidin (Vector Laboratories, Burlingame, CA). An Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays. Probe cell intensity data (Affymetrix CEL files) were generated using Affymetrix GeneChip Command Console (AGCC) software. The microarray platform and data have been submitted to the Gene Expression Omnibus public database at NCBI following MIAME guidelines (GEO accession: GSE71416) [26 (link)].
Doumatey A.P., Xu H., Huang H., Trivedi N.S., Lei L., Elkahloun A., Adeyemo A, & Rotimi C.N. (2015). Global Gene Expression Profiling in Omental Adipose Tissue of Morbidly Obese Diabetic African Americans. Journal of endocrinology and metabolism, 5(3), 199-210.
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4

Transcriptome Profiling using Affymetrix Mouse GeneChip

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RNA collected per sample was prepared for hybridization to the Affymetrix Clariom S Mouse GeneChip using protocols recommended by the GeneChip manufacturer (Affymetrix). Quality and quantity of the RNA was ensured beforehand using the Bioanalyzer (Agilent) and NanoDrop (Thermofisher Scientific) respectively. Post hybridization, GeneChips were washed using the Affymetrix Fluidics Station, stained with streptavidin phycoerythrin solution (Molecular Probes), then enhanced by using an antibody solution containing 0.5 mg/mL of biotinylated anti-streptavidin (Vector Laboratories). Post wash and stain, the Affymetrix Gene Chip Scanner 3000 was used to scan the GeneChips and the Affymetrix AGCC software used to generate one CEL file per hybridized RNA.
Bernstock J.D., Totten A.H., Elkahloun A.G., Johnson K.R., Hurst A.C., Goldman F., Groves A.K., Mikhail F.M, & Atkinson T.P. (2019). Recurrent microdeletions at chromosome 2p11.2 are associated with thymic hypoplasia and features resembling DiGeorge Syndrome. The Journal of allergy and clinical immunology, 145(1), 358-367.e2.
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5

Whole-Genome Microarray Analysis Protocol

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Samples were prepared according to Affymetrix protocols (Affymetrix, Inc). DNA quality and quantity was ensured using Bioanalyzer (Agilent, Inc) and NanoDrop (Thermo Scientific, Inc) respectively. Per DNA labeling, 200 nanograms of genomic DNA were used in conjunction with the Affymetrix recommended protocol for CytoScan HD array kit and reagents (catalog# 901835). The hybridization cocktail containing the fragmented and labeled DNAs was hybridized to The Affymetrix CytoScan HD GeneChip. The chips were washed and stained by the Affymetrix Fluidics Station using the standard format and protocols as described by Affymetrix. The probe arrays were stained with streptavidin phycoerythrin solution (Molecular Probes, Carlsbad, CA) and enhanced by using an antibody solution containing 0.5 mg/mL of biotinylated anti-streptavidin (Vector Laboratories, Burlingame, CA). An Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays. The .Cel files were generated from the scanned images using Affymetrix AGCC software and the .cyhd.cychp files were generated by the Chromosome Analysis Suite (ChAS) Version 2.1 software.
All the analyses were done with ChAS default parameters for LOH and Copy Number State (CNS). A description of the samples used for this analysis is listed in Supplementary Table 5.
Arafeh R., Qutob N., Emmanuel R., Keren-Paz A., Madore J., Elkahloun A., Wilmott J.S., Gartner J.J., Di Pizio A., Winograd-Katz S., Sindiri S., Rotkopf R., Dutton-Regester K., Johansson P., Pritchard A., Waddell N., Hill V.K., Lin J.C., Hevroni Y., Rosenberg S.A., Khan J., Ben-Dor S., Niv M.Y., Ulitsky I., Mann G.J., Scolyer R.A., Hayward N.K, & Samuels Y. (2015). Recurrent inactivating RASA2 mutations in melanoma. Nature genetics, 47(12), 1408-1410.
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6

Murine Microarray Transcriptome Analysis

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Three separate MTT cell samples were prepared according to Affymetrix protocols (Affymetrix, Inc.). RNA quality and quantity was ensured using a Bioanalyzer (Agilent, Inc.) and NanoDrop (Thermo Scientific, Inc.) respectively. Per RNA labeling, 200 ng of total RNA was used in conjunction with the Affymetrix recommended protocol for GeneChip 1.0 ST chips.
The hybridization cocktail containing fragmented and labeled cDNAs was hybridized to the Affymetrix Mouse Genome ST 1.0 GeneChip. The chips were washed and stained by the Affymetrix Fluidics Station using the standard format and protocols as described by Affymetrix. The probe arrays were stained with streptavidin phycoerythrin solution (Molecular Probes, Carlsbad, CA) and enhanced using an antibody solution containing 0.5 mg/ml of biotinylated anti-streptavidin (Vector Laboratories, Burlingame, CA). An Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays. Gene expression intensities were calculated using Affymetrix AGCC software.
Partek Genomic Suite was used to RMA normalize (Robust Multichip Analysis), summarize, logtransform the data, and run ANOVA analysis and hierarchical clustering. The Series entry for the murine microarray in the NCBI Gene Expression Omnibus (GEO) database has been approaved with the following number: GSE51832 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE51832).
Giubellino A., Shankavaram U., Bullova P., Schovanek J., Zhang Y., Shen M., Patel N., Elkahloun A., Lee M.J., Trepel J., Ferrer M, & Pacak K. (2014). High-Throughput Screening for the Identification of New Therapeutic Options for Metastatic Pheochromocytoma and Paraganglioma. PLoS ONE, 9(4), e90458.
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