Murine MSCs derived from compact bone at passage 4 were collected for murine CCR7 detection. Splenic cells (SPC) from the same species served as positive controls. Human MSCs derived from bone marrow (hBM-MSCs, Cyagen) or umbilical cord (hUC-MSCs, Cyagen) at passage 5 were obtained for human CCR7 expression analysis. Human peripheral blood cells (hPBC) were served as positive control. The specific PCR primers were listed as followed. Murine CCR7: 5′-CAGCCTTCCTGTGTGATTTC-3′ (forward), 5′-TGGGAGAGGTCCTTGTAGTC-3′ (Reverse); Human CCR7: 5′-CCAGACAGGGGTAGTGCGAG-3′(Forward), 5′-AGGCAGAAGAGTCGCCTATG-3′ (Reverse); Murine GAPDH: 5′-GGAGCGAGACCCCACTAACA-3′ (Forward), 5′-ACATACTCAGCACCGGCCTC-3′ (Reverse); Human GAPDH: 5′-ATGGGGAAGGTGAAGGTCGGAGTCAA-3′ (Forward), 5′-CGGAGGGGCCATCCACAGTCTTCT-3′ (Forward). RT-PCR was performed as described by the manufacture (TOYOBO).
Hbmmscs
Manufactured by Cyagen
Sourced in China
HBMMSCs are human bone marrow-derived mesenchymal stem cells. They are multipotent progenitor cells capable of differentiating into various cell types, including osteoblasts, chondrocytes, and adipocytes.
Automatically generated - may contain errors
Lab products found in correlation
Hucmscs, by Cyagen (1 mentions)
Raw 264, by Merck Group (1 mentions)
Mouse bmmscs, by Cyagen (1 mentions)
Thp 1, by Merck Group (1 mentions)
Ifn γ, by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Minimal essential medium alpha αmem, by Thermo Fisher Scientific (1 mentions)
Raw264.7 cells, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin mixture, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Abcam (1 mentions)
4 protocols using hbmmscs
1
Murine and Human MSCs CCR7 Expression
2
Activation of Murine and Human Macrophages
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Human bone marrow-derived mesenchymal stem cells (hBMMSCs) and mouse BMMSCs were purchased from Cyagen Biosciences Co., Ltd (Guangzhou, China). Mouse macrophage cell line RAW264.7, mouse keratinocyte cell line JB6, human macrophage cell line THP-1 and human epithelial cell line HaCaT cell line were purchased from Beina Biotechnology Co., Ltd (Guangzhou, China). All cells were maintained following the company’s instructions. For in vitro activation, RAW264.7 cells were treated with 1 µg/mL LPS (Sigma-Aldrich) and 20 ng/mL IFN-γ (Sigma-Aldrich) for 12 h. THP-1 cells were treated with 1 µg/mL LPS (Sigma-Aldrich) and 20 ng/mL hIFN-γ (Sigma-Aldrich) for 24 h after pre-treatment with 200 ng/mL Phorbol-12-myristate-13-acetate (PMA) (Sigma-Aldrich) for 6 h.
3
Murine Macrophages and Human MSCs
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The murine-derived macrophage cell line RAW 264.7 cells (RAW cells, Cell Bank, purchased from the Chinese Academy of Sciences) and human bone marrow mesenchymal stem cells (hBMMSCs) were purchased from Cyagen Biosciences Inc. (Guangzhou, China). The RAW 264.7 cells were cultured using the Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco) and antibiotics (100 U/ml of penicillin and 100 mg/ml of streptomycin) (Thermo Fisher Scientific, United States). The hBMMSCs were cultured using Minimal Essential Medium Alpha (α-MEM, Gibco) supplemented with 10% (v/v) FBS and antibiotics (100 U/ml of penicillin and 100 mg/ml of streptomycin).
4
Osteogenic Differentiation of hBMMSCs
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α2M was obtained from Enzo Life Sciences (Farmingdale, NY, USA). hBMMSCs were obtained from Cyagen Biosciences (Guangzhou) Inc. (HUXMA-01001; Guangzhou, China). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin mixture and 0.25% trypsin-EDTA were purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Osteogenic induction medium was purchased from Cyagen Biosciences (Guangzhou) Inc. (GUXMX-90021). Antibodies against Beclin1 (1:1,000; cat. no. 11306-1-AP), microtubule-associated protein 1A/1B (LC-3; 1:1,000; cat. no. 14600-1-AP), sex determining region Y (Sox2; 1:5,000; cat. no. 11064-1-AP), Nanog (1:2,000; cat. no. 14295-1-AP), manganese superoxide dismutase (MnSOD; 1:3,000; cat. no. 24127-1-AP) and GAPDH (1:10,000; cat. no. 60004-1-Ig) were purchased from ProteinTech Group (Chicago, IL, USA). The runt-related transcription factor 2 (RUNX2; 1:1,000; cat. no. 12556) antibody was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), the osteoglycin (OGN; 1:1,000; cat. no. sc-365228) antibody was obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA), and the goat anti-rabbit (1:10,000; cat. no. ab6721) and goat anti-mouse (1:10,000; cat. no. ab6789) horseradish peroxidase (HRP) conjugated secondary antibodies were obtained from Abcam (Cambridge, MA, USA).
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