Nicolet is50 ft ir instrument

All HPLC separations were performed using Waters Alliance 2629 separation module (Milford, MA, USA), connected with an in-line degasser, a quaternary solvent pump, an autosampler, and a thermostated column compartment. A Waters PDA detector (model 2475) was used to record the signals at 254 nm. All the chromatographic separations were carried out by maintaining the column at room temperature. N-Acetylglucosamine functionalized-silica (GlcNAc-silica) slurry was packed in stainless-steel columns using a constant pressure pump from Shandon Southern Products Ltd. (Rincon, Cheshire, UK). Data acquisition was performed using Empower 2 (Build 2154) software (Waters Chromatography), and then the offline chromatographic data were processed using OriginPro v8.5.1 (Origin Lab Corp., Northhampton, MA, USA). Fourier transform infrared (FTIR) analyses were carried out for the characterization of GlcNAc-silica sorbent using attenuated total reflectance mode on a Nicolet IS50 FT-IR instrument from Thermo Scientific Co. (Waltham, MA, USA). Furthermore, the functionalization of silica with surface bound N-acetylglucosamine was assessed via thermogravimetric analysis using a Q-50 thermogravimetric analyzer from TA instruments (New Castle, DE, USA). Approximately 8–10 mg of the samples was heated from 20 °C to 900 °C at a heating rate of 20 °C per min with a 40 mL/min continuous nitrogen gas flow.

The surface morphology of the MBCDs was examined by transmission electron microscopy (TEM, FEI Tecnai 30) and Atomic Force Microscope (AFM, Dimension Fastscan. The phase composition of the MBCDs was characterized by X-ray diffraction (Rigaku D/max 2550 VB + 18 kW with Cu Kα′ radiation) and X-ray photoelectron spectroscopy (PHI Quantera XPS). The Fourier transform infrared (FTIR) spectra of the MBCDs was collected by the Thermo Nicolet iS50 FT-IR instrument (Thermo Scientific, USA). The fluorescence properties were determined by fluorimeter (FluoroMax-4, HORIBA Jobin Yvon, France).