The following fluorochrome-labeled antibodies (Abs) specific to mouse markers and their corresponding isotype controls were purchased from eBioscience (San Diego, CA): PE-conjugated anti-IL-22 (1H8PWSR), APC-conjugated anti-IL-17A (eBio17B7), FITC- or APC-conjugated anti-IFN-γ (XMG1.2), APC-conjugated anti-TCRγδ (eBioGL3), PE-Cy7-conjugated anti-CD3 (17A2), Pacific Blue-conjugated anti-CD4 (GK1.5), PerCp-Cy5.5-conjugated anti-CD8 (53–6.7), FITC- or APC-conjugated anti-NK1.1 (PK136), FITC- or PerCp-Cy5.5-conjugated anti-CD11b (M1/70), FITC-conjugated anti-CD11c (N418), APC-conjugated anti-Gr-1 (clone: RB6-8C5), APC-conjugated anti-RORγt (B2D), Cell proliferation dye eFluor 670 and eFluor 506-conjugated fixable viability dye. Purified anti-CD16/32 (2.4G2) was purchased from BD Pharmingen (San Diego, CA). APC-Cy7-conjugated anti-CD3 (17A2) and Pacific Blue-conjugated anti-CD8 (53–6.7) were purchased from Biolegend (San Diego, CA). Recombinant murine IL-23, IL-22 and GM-CSF were from Peprotech (Rocky Hill, NJ). The all-trans RA was from Enzo Life Sciences (Farmingdale, NY). The Rapamycin, Ly294003, Wortmannin and rabbit mAb β-actin (D6A8), Phospho-p44/42 MAPK (Erk1/2), p44/42 MAPK (Erk1/2), Phospho-IκBα (Ser32) and IκBα (44D4)) were purchased from Cell Signaling Technology (Beverly, MA). Anti-S100A4 Ab (ab27957) was from Abcam (Cambridge, MA).
Iκbα 44d4
Manufactured by Cell Signaling Technology
Sourced in United States
IκBα (44D4) is a monoclonal antibody that recognizes the inhibitor of NF-κB alpha (IκBα) protein. IκBα is a key regulator of the NF-κB signaling pathway, which plays a crucial role in immune response, inflammation, and cell survival.
Automatically generated - may contain errors
Lab products found in correlation
Cell proliferation dye efluor 670, by Thermo Fisher Scientific (1 mentions)
Gm csf, by Thermo Fisher Scientific (1 mentions)
Rapamycin, by Cell Signaling Technology (1 mentions)
Recombinant murine il 23, by Thermo Fisher Scientific (1 mentions)
Wortmannin, by Cell Signaling Technology (1 mentions)
Il 22, by Thermo Fisher Scientific (1 mentions)
P enos, by Cell Signaling Technology (1 mentions)
β actin 13e5, by Cell Signaling Technology (1 mentions)
Phosphorylated akt p akt, by Cell Signaling Technology (1 mentions)
Anti inos, by Abcam (1 mentions)
Ecl plus, by GE Healthcare (1 mentions)
Kenpaullone, by Merck Group (1 mentions)
Tumor necrosis factor (tnf), by Merck Group (1 mentions)
Bcl 3, by Santa Cruz Biotechnology (1 mentions)
Horseradish peroxidase coupled secondary antibody, by Cell Signaling Technology (1 mentions)
P ikkα β, by Cell Signaling Technology (1 mentions)
Staurosporine, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Limulus amoebocyte lysate assay, by Lonza (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
4 protocols using iκbα 44d4
1
Multiparameter Flow Cytometry of Immune Cells
2
Protein Expression Analysis in Aortic and Cardiac Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The thoracic aorta and heart were homogenized and lysed, and proteins (25 μg) were separated on 10% SDS-PAGE. Blots were probed with the following antibodies: phosphorylated-AKT (p-AKT) (rabbit anti-rat AKT, phosphorylated (Ser473)), AKT (AKT-pan (C67E7)), phosphorylated endothelial nitric oxide synthase (p-eNOS) (rabbit anti-rat eNOS, phosphorylated (Ser1177)), endothelial nitric oxide synthase (eNOS) (rabbit anti-rat eNOS), phosphorylated NF-κB (Phospho-NF-κB p65 (Ser536) (93H1)) and IκBα ((44D4)), all from Cell Signaling Technology, Saint Quentin Yvelines, France; anti-iNOS (Abcam, Paris, France) and β-actin (13E5) (Cell Signaling Technology, Saint Quentin Yvelines, France). Proteins were transferred onto nitrocellulose membranes after which bound antibodies were detected with a secondary peroxidase-conjugated anti-rabbit IgG (Promega, Madison, WI, USA). The blots were visualized using an enhanced chemiluminescence system (ECL Plus; Amersham, GE Healthcare Europe, Velizy-Villacoublay, France).
3
Kinase Inhibition for Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For stimulation and long-term incubation experiments, TNF from Sigma Aldrich was used. To suppress protein degradation in cell lysates, the complete ethylenediaminetetraacetic acid-free protease inhibitor cocktail (Roche, Rotkreuz, Switzerland) was applied. Inhibitor experiments were performed using the kinase inhibitor Staurosporine (Stauro) and the GSK3α/β inhibitor Kenpaullone (Ken; Sigma Aldrich). For Western blotting, antibodies specific for PKCβ (D3E70), p-PKCα/βII (Thr638/641), GSK3β (27C10), p-GSK3α/β (Ser21/9), p65 (L8F6), p-p65 (Ser536), IKKα, p-IKKα/β (Ser176/180), IκBα (44D4; Cell Signalling, Danvers, USA), B cell lymphoma (Bcl) 3 (150–3.5; Santa Cruz Biotechnology, Santa Cruz, USA), actin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Sigma-Aldrich) were used. Horseradish peroxidase-coupled secondary antibodies were purchased from Cell Signalling or Santa Cruz. All media and reagents were of the best available grade and routinely tested for endotoxins with the Limulus Amoebocyte Lysate assay (Lonza, Basel, Switzerland).
4
Evaluating Anti-Inflammatory Agents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TSA and SAHA were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum were purchased from Gibco-BRL, Life Technologies (Carlsbad, CA, USA). Malondialdehyde (MDA), nitric monoxide (NO), and myeloperoxidase (MPO) detection kits were purchased from Jiancheng Bioengineering Institute (Nanjing, China). Fluorescent-labeled anti-GR1 and anti-CD3ε MAb were purchased from BD Biosciences (San Jose, CA, USA). Human and mouse interleukin (IL)-6, tumor necrosis factor-α (TNF-α) and IL-8/or KC enzyme-linked immunosorbent assay (ELISA) kits were purchased from Shanghai ExCell Biology Inc., (Shanghai, China) or eBioscience (San Diego, CA, USA). The antibodies against IκBα (44D4), total Stat3 (D3Z2G), phospho-Stat3 (Tyr705; D3A7), cyclo-oxygenase-2 (COX-2; D5H5), ERK (137F5), phospho-ERK (D13.14.4E), JNK, phospho-JNK (81E11), p-38 (D13E1), phospho-p-38 (D3F9), HDAC2 (3F3), acetyl-histone H3 (Ac-H3; C5B11), and GAPDH were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!