Murine il 1β

Manufactured by R&D Systems

Murine IL-1β is a recombinant mouse interleukin-1 beta protein. Interleukin-1 beta is a pro-inflammatory cytokine produced by various cell types, including macrophages, dendritic cells, and epithelial cells.

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Lab products found in correlation

Lipopolysaccharides (lps), by Merck Group (2 mentions) Human g csf, by R&D Systems (1 mentions) Nω nitro l arginine methyl ester hydrochloride l name, by Merck Group (1 mentions) Phenol red free dmem, by Thermo Fisher Scientific (1 mentions) Murine tnf α, by R&D Systems (1 mentions) Cell recovery solution, by BD (1 mentions) Murine ifn γ, by Thermo Fisher Scientific (1 mentions) Cleaved casp7, by Cell Signaling Technology (1 mentions) Af 401 na, by Cell Signaling Technology (1 mentions) Ag 20b 0042 c100, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Recombinant murine IL-1β Human or murine IL-1β ELISA kits Murine IL-1α and IL-1β ELISA kits IL-1β

5 protocols using murine il 1β

Induction of mBSA Arthritis in Mice

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For the induction of mBSA arthritis models (23 (link)), mice were injected with 200 μg of mBSA in 10 μl into the left knee, and saline was injected into the contralateral knee, followed by s.c. injection on days 0–2 of either murine IL-1β (250 ng; R&D Systems), human G-CSF (250 ng; R&D Systems), or saline. In some experiments, indomethacin (1 mg/kg) or the cyclooxygenase (COX)-2 selective inhibitor, SC581258 (1 mg/kg), was given i.p. once pain was evident and daily thereafter.

Lee M.C., McCubbin J.A., Christensen A.D., Poole D.P., Rajasekhar P., Lieu T., Bunnett N.W., Garcia-Caraballo S., Erickson A., Brierley S.M., Saleh R., Achuthan A., Fleetwood A.J., Anderson R.L., Hamilton J.A, & Cook A.D. (2017). G-CSF Receptor Blockade Ameliorates Arthritic Pain and Disease. Journal of immunology (Baltimore, Md. : 1950), 198(9), 3565-3575.

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Preparation of Murine Inflammatory Agents

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Murine IL-1β (R&D Systems, Minneapolis, MN), murine TNF-α (R&D Systems), and l-leucine methyl ester hydrochloride (l-LME; Sigma, St. Louis, MO) were dissolved in PBS. LPS (Sigma) was dissolved in PBS + 0.1% bovine serum albumin (BSA). Nω-Nitro-l-arginine methyl ester hydrochloride (l-NAME; Sigma) was dissolved in phenol red-free DMEM (#31053, Life Technologies, Carlsbad, CA) supplemented with l-glutamine (2 mM) and gentamicin (50 μg/mL).

Krasnow S.M., Knoll J.G., Verghese S.C., Levasseur P.R, & Marks D.L. (2017). Amplification and propagation of interleukin-1β signaling by murine brain endothelial and glial cells. Journal of Neuroinflammation, 14, 133.

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Isolation and Immunoblotting of Intestinal Crypts

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Colon sections (3 cm) were untreated or treated with 1 μg/ml LPS (Sigma; E. coli 0111.B4); 20 ng/ml murine IFNγ (Peprotech; 315–05); or 20 ng/ml murine IL-1β (R&D; 401-ML-005) for 24 h. Sections were agitated (4 °C, 2 h) in 0.5 ml Cell Recovery Solution (BD). Crypts were dislodged by vigorous vortex and separated from the lamina propria fraction by aspiration. Fractions were centrifuged at (350 × g, 10 min), pellets lysed (100 μl ice-cold RIPA buffer) and protein concentration was calculated (BCA method) before immunoblotting.

Flood B., Manils J., Nulty C., Flis E., Kenealy S., Barber G., Fay J., Mills K.H., Kay E.W, & Creagh E.M. (2018). Caspase-11 regulates the tumour suppressor function of STAT1 in a murine model of colitis-associated carcinogenesis. Oncogene, 38(14), 2658-2674.

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Protein Extraction and Western Blot Analysis

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Protein extraction from macrophages was performed using TRIzol reagent according to the manufacturer's instructions. Briefly, after phase separation using chloroform, 100% ethanol was added to the interphase/phenol–chloroform layer to precipitate genomic DNA. Subsequently, the phenol–ethanol supernatant was mixed with isopropanol to isolate proteins. The Bradford method was used to determine protein concentrations in cell lysates. Equal amounts of protein or supernatants were separated by 13.5% SDS–PAGE and transferred to a polyvinylidene fluoride (PVDF) membrane. Membranes were incubated overnight with antibodies against CASP11 (Cell Signaling, 14340), murine CASP1 (Adipogen, AG‐20B‐0042‐C100), human cleaved CASP1 (Cell Signaling Technology, 4199), human CASP1 (Cell Signaling Technology, 2225), cleaved CASP7 (Cell Signaling Technology, 9491), human CASP4 (MBL, M0293), murine IL‐1β (R&D Systems, AF‐401‐NA), and GAPDH (Cell Signaling Technology, 2118). Corresponding secondary antibodies conjugated with horseradish peroxidase and in combination with enhanced chemiluminescence reagent were used to visualize protein bands. Densitometry analyses were performed by normalizing target protein bands to their respective loading control (GAPDH) using ImageJ software as previously described 8, 9.

Krause K., Daily K., Estfanous S., Hamilton K., Badr A., Abu Khweek A., Hegazi R., Anne M.N., Klamer B., Zhang X., Gavrilin M.A., Pancholi V, & Amer A.O. (2019). Caspase‐11 counteracts mitochondrial ROS‐mediated clearance of Staphylococcus aureus in macrophages. EMBO Reports, 20(12), e48109.

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Vulvar Biopsy Cytokine Assay

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Six mm punch biopsies were collected, encompassing the entire vulvar area immediately posterior to the vaginal opening, and each biopsy tissue was oriented to permit non-tangential cross-sectioning and was equally bisected to create two samples. Each biopsy half was washed several times in sterile saline prior to being placed in a 24 well plate. Twelve mice donated a total of 24 biopsies pieces, which were divided among treatments; 3 pieces from different individuals (n=3) were used for each treatment. Each well, containing a single biopsy piece, was flooded with 0.5 ml serum-free MEM, supplemented with GlutaMAX, gentamicin, and antibiotic/antimycotic (Gibco). 3D cultures were pre-treated with lipoxin A₄, resolvin D₂, or maresin 1 at a concentration of 1, 10, or 100 nM for 24 h. Each treatment was applied to three pieces of biopsy tissue. After 24 h, cells were treated a second time with lipoxin A₄, resolvin D₂, or maresin 1 30 minutes prior to treatment with 10 pg/ml murine IL-1β (R&D Systems) for another 24 h before harvesting supernatants and assaying for PGE₂, as described earlier.

Falsetta M.L., Wood R.W., Linder M.A., Bonham A.D., Honn K.V., Maddipati K.R., Phipps R.P., Haidaris C.G, & Foster D.C. (2021). Specialized pro-resolving mediators reduce pro-nociceptive inflammatory mediator production in models of localized provoked vulvodynia. The journal of pain, 22(10), 1195-1209.

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