the following antibodies were used at a concentration of 1 μg Ab/mL sample: EpCAM (Biolegend), CD151 (Miltenyi), and EGFR (Thermo Fisher). The isotype controls used were the Biolegend IgGk2b (to match EpCAM), Miltenyi REA Isotype control (to match CD151), and the Biolegend IgGK1 (to match EGFR). Notably, the liver cancer EV subpopulation isolation required filtering with a .45 μm filter unit (GE Whatman) to achieve strong specificity versus isotype control. Samples were run at a flow rate of = 2.5 mL/hr.
Iggk2b
Manufactured by Miltenyi Biotec
The IgGk2b is a laboratory equipment product offered by Miltenyi Biotec. It is a type of antibody that can be used for various research and analysis applications. The core function of the IgGk2b is to facilitate the detection and quantification of specific target molecules or cell types in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Cd151, by Miltenyi Biotec (4 mentions)
Epcam, by Miltenyi Biotec (4 mentions)
Epcam, by BioLegend (4 mentions)
Rea isotype control, by BioLegend (2 mentions)
Cd151, by BioLegend (2 mentions)
Iggk1, by BioLegend (2 mentions)
Tspan8, by Miltenyi Biotec (2 mentions)
One step biotinylation kit, by Miltenyi Biotec (2 mentions)
Rea isotype control, by Novus Biologicals (2 mentions)
Cd151, by Novus Biologicals (2 mentions)
Tspan8, by Novus Biologicals (2 mentions)
Rabbit igg, by Novus Biologicals (2 mentions)
4 protocols using iggk2b
1
Liver Cancer EV Subpopulation Isolation
2
Multiparameter Flow Cytometry of Cell Surface Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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the following antibodies were used at a concentration of 1 μg Ab/mL sample: EpCAM (Biolegend), EGFR (Novus, biotinylated via the Miltenyi one-step biotinylation kit), CD151 (Miltenyi), and Tspan8 (Miltenyi). The isotype controls used were the Biolegend IgGk2b (to match EpCAM), Miltenyi REA Isotype control (to match CD151 and Tspan8), and Novus Rabbit IgG (to match EGFR). Samples were run at a flow rate of ξ = 2.5 mL/hr.
3
Liver Cancer EV Subpopulation Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies were used at a concentration of 1 µg Ab/mL sample: EpCAM (Biolegend), CD151 (Miltenyi), and EGFR (Thermo Fisher). The isotype controls used were the Biolegend IgGk2b (to match EpCAM), Miltenyi REA Isotype control (to match CD151), and the Biolegend IgGK1 (to match EGFR). Notably, the liver cancer EV subpopulation isolation required filtering with a 0.45 µm filter unit (GE Whatman) to achieve strong specificity versus isotype control. Samples were run at a flow rate of ɸ = 2.5 mL/h.
4
Immunophenotyping of Cell Surface Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies were used at a concentration of 1 µg Ab/mL sample: EpCAM (Biolegend), EGFR (Novus, biotinylated via the Miltenyi one-step biotinylation kit), CD151 (Miltenyi), and Tspan8 (Miltenyi). The isotype controls used were the Biolegend IgGk2b (to match EpCAM), Miltenyi REA Isotype control (to match CD151 and Tspan8), and Novus Rabbit IgG (to match EGFR). Samples were run at a flow rate of ɸ = 2.5 mL/h.
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