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Anti human cd68 pecy7

Manufactured by BioLegend

Anti-human CD68-PECy7 is a fluorochrome-conjugated antibody that binds to the CD68 antigen expressed on human monocytes, macrophages, and dendritic cells. It can be used for the identification and enumeration of these cell populations in flow cytometry applications.

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2 protocols using anti human cd68 pecy7

1

Detecting CD68 in Leukemia Cells

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Leukaemia cell lines were blocked in FACS buffer (PBS + 0.5% FBS) containing human Fc block (BD Biosciences; 564220) for 20 min at room temperature. Cells were fixed, permeabilized using the BD Cytofix/Cytoperm fixation/permeabilization kit (BD Bioscience) and later stained with anti-human CD68-PECy7 (BioLegend; 333816). Sample acquisition was performed on a BD LSR II flow cytometer and the mean fluorescence intensities (MFI) were calculated using FlowJo 9.3 software for Mac OS X (TreeStar).
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2

Detecting CD68 in Leukemia Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Leukaemia cell lines were blocked in FACS buffer (PBS + 0.5% FBS) containing human Fc block (BD Biosciences; 564220) for 20 min at room temperature. Cells were fixed, permeabilized using the BD Cytofix/Cytoperm fixation/permeabilization kit (BD Bioscience) and later stained with anti-human CD68-PECy7 (BioLegend; 333816). Sample acquisition was performed on a BD LSR II flow cytometer and the mean fluorescence intensities (MFI) were calculated using FlowJo 9.3 software for Mac OS X (TreeStar).
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