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Mouse vegf quantikine elisa kit

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The Mouse VEGF Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure mouse Vascular Endothelial Growth Factor (VEGF) levels in cell culture supernates, serum, and plasma. The kit uses a monoclonal antibody specific for mouse VEGF coated on a microplate to capture the VEGF present in the sample. An enzyme-linked polyclonal antibody specific for mouse VEGF is then added, and after a series of washes, a substrate solution is added to the wells and color develops in proportion to the amount of mouse VEGF bound.

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Lab products found in correlation

Human vegf quantikine elisa kit, by R&D Systems (3 mentions) Protease inhibitor cocktail, by Merck Group (2 mentions) Mouse rat pdgf bb quantikine elisa kit, by R&D Systems (2 mentions) Ratlapstm eia kit, by Immunodiagnostic Systems (2 mentions) Crosslaps for culture elisa kit, by Immunodiagnostic Systems (2 mentions) Mouse osteocalcin eia kit, by Biomedical Technologies (2 mentions) Mouse il 6 quantikine elisa kit, by R&D Systems (2 mentions) Igepal ca 630, by Merck Group (1 mentions) Facsversetm flow cytometer, by BD (1 mentions) Mouse epo quantikine elisa kit, by R&D Systems (1 mentions) Protein assay dye reagent concentrate, by Bio-Rad (1 mentions) M per buffer, by Thermo Fisher Scientific (1 mentions) Quant it picogreen dsdna assay kit, by Thermo Fisher Scientific (1 mentions) Serum collection tube, by Sarstedt (1 mentions) Multimode plate reader, by Agilent Technologies (1 mentions) Mouse il 6 elisa ready set go, by Thermo Fisher Scientific (1 mentions) Tgf β quantikine elisa kit, by R&D Systems (1 mentions) Mouse il 1β il 1f2 duoset elisa, by R&D Systems (1 mentions) Mouse tnf α duoset elisa, by R&D Systems (1 mentions) Mouse il 10 elisa kit, by ABclonal (1 mentions)

55 protocols using mouse vegf quantikine elisa kit

1

Cytokine profiling of murine liver

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Snap-frozen liver tissues were homogenized using the mixer mill MM 301 (Retsch, Haan, Germany) and subsequently reconstituted in PBS + 1% IGEPAL ® CA-630 (Sigma Aldrich), as well as a protease inhibitor cocktail (Sigma-Aldrich) in order to avoid proteolytic degradation. After centrifugation at 10,000× g, supernatants were kept at −80°C until use. For determination of liver inflammation, the LEGENDplexTM Murine Inflammation Panel (Bio-Legend) was utilized according to the manufacturer`s instructions. Data acquisition was carried out on a FACS VerseTM flow cytometer (Beckton Dickinson) and analysis was done using the provided software (VigeneTech). Additionally, murine VEGF concentrations were determined by utilizing the Mouse VEGF Quantikine ELISA Kit (R&D Systems) according to the manufacturer’s instructions. Photometric measurements were conducted on an InfiniteR 200PRO Microplate Reader (Tecan, Maennedorf, Switzerland). Detected cytokine concentrations were normalized to corresponding protein values of the liver homogenates.
Miarka L., Hauser C., Helm O., Holdhof D., Beckinger S., Egberts J.H., Gundlach J.P., Lenk L., Rahn S., Mikulits W., Trauzold A, & Sebens S. (2019). The Hepatic Microenvironment and TRAIL-R2 Impact Outgrowth of Liver Metastases in Pancreatic Cancer after Surgical Resection. Cancers, 11(6), 745.
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2

Quantifying Bone and Angiogenic Markers

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We performed osteocalcin or CTX ELISA analysis of serum using a mouse osteocalcin EIA kit (Biomedical Technologies Inc.) or a RatLapsTM EIA kit (Immunodiagnosticsystems). We performed CTX ELISA analysis of conditioned media using a CrossLaps® for Culture ELISA kit (Immunodiagnosticsystems). We performed PDGF-BB or VEGF ELISA analysis of conditioned medium, serum or bone marrow supernatant using a Mouse/Rat PDGF-BB Quantikine ELISA kit or a Mouse VEGF Quantikine ELISA Kit (R&D Systems). We did all ELISA assays according to the manufacturers' instructions.
Xie H., Cui Z., Wang L., Xia Z., Hu Y., Xian L., Li C., Xie L., Crane J., Wan M., Zhen G., Bian Q., Yu B., Chang W., Qiu T., Pickarski M., Duong L.T., Windle J.J., Luo X., Liao E, & Cao X. (2014). PDGF-BB secreted by preosteoclasts induces CD31hiEmcnhi vessel subtype in coupling osteogenesis. Nature medicine, 20(11), 1270-1278.
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3

Quantifying Bone and Angiogenic Markers

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We performed osteocalcin or CTX ELISA analysis of serum using a mouse osteocalcin EIA kit (Biomedical Technologies Inc.) or a RatLapsTM EIA kit (Immunodiagnosticsystems). We performed CTX ELISA analysis of conditioned media using a CrossLaps® for Culture ELISA kit (Immunodiagnosticsystems). We performed PDGF-BB or VEGF ELISA analysis of conditioned medium, serum or bone marrow supernatant using a Mouse/Rat PDGF-BB Quantikine ELISA kit or a Mouse VEGF Quantikine ELISA Kit (R&D Systems). We did all ELISA assays according to the manufacturers' instructions.
Xie H., Cui Z., Wang L., Xia Z., Hu Y., Xian L., Li C., Xie L., Crane J., Wan M., Zhen G., Bian Q., Yu B., Chang W., Qiu T., Pickarski M., Duong L.T., Windle J.J., Luo X., Liao E, & Cao X. (2014). PDGF-BB secreted by preosteoclasts induces CD31hiEmcnhi vessel subtype in coupling osteogenesis. Nature medicine, 20(11), 1270-1278.
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4

VEGF Secretion in Hypoxic PBMCs

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PBMCs were obtained using the Ficoll-Paque centrifugation (GE Healthcare, 17–5446–02), according to the manufacturer’s instructions. Primary monocytes were isolated from the PBMCs by MACS CD11b (Miltenyi Biotec, 130-049-601).
To investigate the secretion of VEGF from PBMCs after OGD, the conditioned media from PBMCs was used. Briefly, after OGD was performed using primary PBMCs, the level of VEGF in the conditioned media was measured using the human VEGF Quantikine® ELISA Kit (DVE00, R&D Systems, Minneapolis, MN, USA) and the mouse VEGF Quantikine® ELISA Kit (RRV00, R&D Systems)45 (link), according to the manufacturer’s instructions (N = 4~6).
Hatakeyama M., Kanazawa M., Ninomiya I., Omae K., Kimura Y., Takahashi T., Onodera O., Fukushima M, & Shimohata T. (2019). A novel therapeutic approach using peripheral blood mononuclear cells preconditioned by oxygen-glucose deprivation. Scientific Reports, 9, 16819.
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5

Quantifying VEGF in Mouse Ascites and Sera

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Blood samples were collected by jugular vein puncture before the mice were killed. After the mice were killed, the VEGF concentrations in the ascites and sera were measured using a mouse VEGF Quantikine ELISA kit (MMV00; R&D Systems) according to the manufacturer’s instructions.
Kawata Y., Nagasaka K., Oda K., Makii C., Takeuchi M., Oki S., Honjo H., Kojima M., Miyagawa Y., Taguchi A., Tanikawa M., Sone K., Hiraike H., Matsumoto Y., Wada‐Hiraike O., Ayabe T., Osuga Y, & Fujii T. (2020). Effect of murine double‐minute 2 inhibitors in preclinical models of advanced clear cell carcinomas originating from ovaries and kidneys. Cancer Science, 111(10), 3824-3834.
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6

Quantitative ELISA for Plasma and Tissue Proteins

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The following commercially available ELISA kits were used to measure protein levels in plasma and tissue lysates: mouse VEGF Quantikine ELISA kit (R&D Systems; MV00), human sFlt Quantikine ELISA kit (R&D Systems; DVR100B), and mouse Epo Quantikine ELISA kit (R&D Systems; MEP00). Blood for plasma collection was drawn via the tail vein with 12 mM EDTA or heparin as an anticoagulant and then centrifuged for 20 min at 2,000 g. Tissue lysates for ELISA were homogenized in PBS using a Next Advance Bullet Blender homogenizer, stored overnight at −20°C, and lysed with two freeze–thaw cycles followed by centrifugation for 5 min at 5,000 g and collection of the supernatant. The Bradford assay was performed to standardize tissue lysate concentrations according to the manufacturer’s instructions (Bio-Rad Protein Assay Dye Reagent Concentrate; 500-0006). ELISAs were read at 450 nm with a reference value of 540 nm using a Tecan Infinite f200 Pro 96-well plate reader.
Greenwald A.C., Licht T., Kumar S., Oladipupo S.S., Iyer S., Grunewald M, & Keshet E. (2019). VEGF expands erythropoiesis via hypoxia-independent induction of erythropoietin in noncanonical perivascular stromal cells. The Journal of Experimental Medicine, 216(1), 215-230.
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7

VEGF-A Secretion in Bbs4 Knockout Islets

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Isolated islets from control and Bbs4-/- animals were cultured overnight. 30 islets from each group were picked into four well plates containing 450 μl culture medium per well. 72 hr later, supernatants were collected and stored at −80°C. Islets were collected and lysed in 50 μl M-PER buffer (Thermo Fisher Scientific, USA), for quantification of DNA contents by Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, USA) later according to manufacturer’s instructions. Secreted VEGF-A in the supernatants was measured with mouse VEGF Quantikine ELISA Kit (R&D Systems, USA), according to manufacturer’s instructions.
Xiong Y., Scerbo M.J., Seelig A., Volta F., O'Brien N., Dicker A., Padula D., Lickert H., Gerdes J.M, & Berggren P.O. (2020). Islet vascularization is regulated by primary endothelial cilia via VEGF-A-dependent signaling. eLife, 9, e56914.
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8

Serum VEGF and TNF Quantification

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Blood was collected into serum collection tubes (Sarstedt, #41.1500.005) by cardiac puncture and centrifuged at 13,000 rpm for 15 min at 4°C to isolate serum. VEGF and TNF levels were quantified using a mouse VEGF Quantikine ELISA kit and TNF Quantikine ELISA kit (R&D Systems), respectively, according to the manufacturer’s instructions. All samples were analyzed in duplicate.
Branche E., Tang W.W., Viramontes K.M., Young M.P., Sheets N., Joo Y., Nguyen A.V, & Shresta S. (2018). Synergism Between the Tyrosine Kinase Inhibitor Sunitinib and Anti-TNF Antibody Protects Against Lethal Dengue Infection. Antiviral research, 158, 1-7.
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9

VEGF Quantification in Mouse Retina

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Mouse retinal tissues or mRMECs were homogenized or lysed in an ice-cold lysis buffer containing a 1X protease inhibitor cocktail and 1 mM PMSF by sonication on ice. Protein concentrations were determined using BCA protein assay reagent. The VEGF levels were measured using mouse VEGF Quantikine ELISA kit (R&D Systems) as instructed in the manual. The amount of VEGF was normalized with total protein concentrations.
Chen S., Sun Q., Sun D., Willette-Brown J., Anderson M.J., Gu Q., Lewandoski M., Hu Y., Zhu F., Wei F, & Zhang J. (2020). C-CBL is required for inhibition of angiogenesis through modulating JAK2/STAT3 activity in ROP development. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 132, 110856.
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10

Measuring Lung Cell Cytokine Levels

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The concentrations of VEGF, transforming growth factor-beta (TGF-β), interleukin 6 (IL-6), interleukin 1-beta (IL-1β), and tumor necrosis factor-alpha (TNF-α) expressed by lung cells were measured by performing ELISA using the following commercial kits: mouse VEGF Quantikine ELISA Kit, cat# MMV00; mouse/rat/porcine/canine TGF-β Quantikine ELISA Kit, cat# MB100B; mouse IL-1β/IL-1F2 DuoSet ELISA, cat# DY401-05; and mouse TNF-α DuoSet ELISA, cat# DY410-05 (R&D Systems, Minneapolis, MN, USA) and mouse IL-6 ELISA Ready-SET-Go, cat#: 88–7064 (eBioscience, San Diego, CA, USA). ELISA plates were read on a multimode plate reader (BioTek, Winooski, VT, USA). For ELISA, the number of animals used per group was as follows: normal control (n = 4), day 0 (n = 4), day 1 (n = 4), day 3 (n = 4), day 7 (n = 6), and day 14 (n = 6).
Lin C.K., Huang T.H., Yang C.T, & Shi C.S. (2021). Roles of lung-recruited monocytes and pulmonary Vascular Endothelial Growth Factor (VEGF) in resolving Ventilator-Induced Lung Injury (VILI). PLoS ONE, 16(3), e0248959.
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