Cd103

Manufactured by Thermo Fisher Scientific

Sourced in United States

The CD103 is a lab equipment product from Thermo Fisher Scientific. It is a device used for the detection and analysis of specific cell surface markers. The core function of the CD103 is to provide researchers with the ability to identify and quantify cell populations expressing the CD103 antigen.

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Lab products found in correlation

Cd11b, by Thermo Fisher Scientific (3 mentions) Mhcii, by BioLegend (2 mentions) Cd11b, by BioLegend (2 mentions) Granzyme b, by Thermo Fisher Scientific (2 mentions) Cd62l, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Cd8 53 6, by BD (1 mentions) Cd117 2b8, by BD (1 mentions) Mhcii, by Thermo Fisher Scientific (1 mentions) Cd4 gk1, by BD (1 mentions) B220 ra3 6b2, by Thermo Fisher Scientific (1 mentions) Cd49b dx5, by Thermo Fisher Scientific (1 mentions) Siglecf e50 2440, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Diva software, by BD (1 mentions) Facscanto, by BD (1 mentions) P2ry12, by BioLegend (1 mentions) Ifn γ, by BioLegend (1 mentions) Lsrfortossa flow cytometer, by BD (1 mentions) Granzyme b, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Anti-CD103 CD103-PE CD103 (clone 2E7, CD103-PE-Cy7 Biotinylated anti-CD103 antibody CD103-FITC CD103 (2E7), CD103-APC CD103 (B-Ly7, Anti-CD103-APC

14 protocols using cd103

Comprehensive Flow Cytometry Panels

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There were two flow cytometry panels used; a DC and a lymphocyte panel. These panels were further subdivided into cytokine measures to identify which cells were releasing cytokines as well as markers of cell activation. The fluorochrome-conjugated antibodies used in the DC cytokine panel were: Fixable Viability Stain 450, CD45 BV510, HLA-DR BV605, CD56 PerCP-Cy5.5, CD123 PE, CD14 Pe-Cy5, CD19 PE-Cy5, CD11c AF700, CD3 APC-H7, IL-6 FITC, IFN-γ PE-Cy7, IFN-α AF647 and IL-10 PE-CF594. Antibodies used in the DC activation marker panel were: Fixable Viability Stain 450, CD45-BV510, CD56 PerCP-Cy5.5, CD123 PE, CD14 Pe-Cy5, CD19 PE-Cy5, CD11c AF700, CD3 APC-H7, HLA-DR APC, CD80 PE-Cy7, CD86 PE-CF594 and HLA-A, B, C FITC. Antibodies used in the lymphocyte cytokine panel were: Fixable Viability Stain 450, CD45 BV510, CD56 BV605, CD4 PerCP-Cy5.5, FoxP3 PE, CD25 APC, CD8 AF700, CD3 APC-H7, IL-6 FITC, IFN-γ PE-Cy7 and IL-10 PE-CF594. Antibodies used in the lymphocyte activation marker panel were: Fixable Viability Stain 450, CD45 BV510, CD56 BV605, CD4 PerCP-Cy5.5, FoxP3 PE, CD25 APC, CD8 AF700, CD3 APC-H7, CD103 PE-Cy7, CTLA-4 PE-CF594 and GITR AF488. All were from BD Bioscience, CA, USA except CD14, CD19, GITR and CD103 (ebioscience, USA). Appropriate controls were used in all experiments.

Tolosa J.M., Parsons K.S., Hansbro P.M., Smith R, & Wark P.A. (2015). The Placental Protein Syncytin-1 Impairs Antiviral Responses and Exaggerates Inflammatory Responses to Influenza. PLoS ONE, 10(4), e0118629.

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Multiparametric Flow Cytometry Analysis

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Ears were collected and processed as described^{54 (link)}. All cells were first pre-incubated with anti-mouse CD16/CD32 for blockade of Fc γ receptors, then were washed and incubated for 40 min with the appropriate monoclonal antibody conjugates in a total volume of 200 μl PBS containing 2 mM EDTA and 2% (vol/vol) bovine serum. DAPI (Invitrogen) was used to distinguish live cells from dead cells during cell analysis. Stained cells were analyzed on a FACS Canto or LSRII machine using the Diva software (BD Bioscience). Data were analyzed with FlowJo software (TreeStar). The following fluorochrome-conjugated anti-mouse antibodies were used at indicated dilutions: CD103 (2E7, 1:200), CD11c (N418, 1:200), CD24 (30-F11, 1:200), CD11b (M1/70, 1:200), MHC-II (M5/114.15.2, 1:400), CD45 (30-F11, 1:200), CD64 (X54-5/7.1, 1:200), Ly6C (HK1.4, 1:200), TCRb (H57-597, 1:200), CD3 (145-2C11, 1:200), TCRgd (EBIOGL3, 1:200), B220 (RA3-6B2, 1:200), CD49b (DX5, 1:200) and FCeR1 (MAR-1, 1:200) were from eBioscience; Siglec-F (E50-2440, 1:200), Ly6G (1A8, 1:200), CD117 (2B8, 1:200), CD8 (53-6.7, 1:200), and CD4 (GK1.5, 1:200) were from BD Bioscience.

Chen L., Deshpande M., Grisotto M., Smaldini P., Garcia R., He Z., Gulko P.S., Lira S.A, & Furtado G.C. (2020). Skin expression of IL-23 drives the development of psoriasis and psoriatic arthritis in mice. Scientific Reports, 10, 8259.

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Multiparameter flow cytometry analysis of immune cell subsets

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All antibodies used at 1:200: CD11b (Clone M1/70, Biolegend, Cat 10137), CD45 (Clone 30‐F11, eBioscience, Cat 56–0451–82), MHCII (Clone M5/114.15.2, Biolegend, Cat 107626), CD80 (Clone 16‐10A1, Biolegend, Cat 104706), CD103 (Clone 2E7, eBioscience, Cat 48‐1031‐80), CD8a (Clone 53‐6.7, Biolegend, Cat 100712), CD4 (Clone RM4‐5, BD Biosciences, Cat 550954), CD44 (Clone IM7, Biolegend Cat 103011), P2RY12 (Clone S16007D, Biolegend Cat 848006), IFNγ (Clone XMG1.2, Biolegend Cat 505826), CD68 (Clone FA‐11, Biolegend Cat 137017). WNV‐specific CD8+ T cells were identified with fluorescent‐labeled immunodominant Db‐restricted NS4B peptide.

Funk K.E., Arutyunov A.D., Desai P., White J.P., Soung A.L., Rosen S.F., Diamond M.S, & Klein R.S. (2021). Decreased antiviral immune response within the central nervous system of aged mice is associated with increased lethality of West Nile virus encephalitis. Aging Cell, 20(8), e13412.

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Flow Cytometric Analysis of Immune Cells

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The following fluorescent mouse Abs from Biolegend (San Diego, CA, USA) were used for flow cytometry analysis: CD3, CD4, CD8, CD103, CD25, CD62L, CD69, CD86, CD138, Foxp3, and Ki-67; from eBioscience (San Diego, CA, USA): B220, granzyme B, perforin; from Santa Cruz (Dallas, TX, USA): granzyme A. Cell subsets were stained with mAbs and isotype control as indicated above and analyzed on BD LSRFortossa™ flow cytometer (BD Biosciences, San Diego, CA, USA) using FACSDiva Software (BD Biosciences). For intracellular staining, such as Foxp3, Granzyme A, GranzymeB, perforin, and Ki-67, cells were first stained with surface marker, and further fixed and permeabilized for intracellular staining using Fix and Perm (eBioscience). Final plmiceot/histogram figures were prepared using FlowJo Software (Tree Star, Ashland, OR, USA).

Zhong H., Liu Y., Xu Z., Liang P., Yang H., Zhang X., Zhao J., Chen J., Fu S., Tang Y., Lv J., Wang J., Olsen N., Xu A, & Zheng S.G. (2018). TGF-β-Induced CD8+CD103+ Regulatory T Cells Show Potent Therapeutic Effect on Chronic Graft-versus-Host Disease Lupus by Suppressing B Cells. Frontiers in Immunology, 9, 35.

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Parotid lymph node cell analysis

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A single-cell suspension was made by squeezing the right superficial parotid lymph node and suspending the cells in PBS from all animals immediately after euthanasia. The cell suspension was passed through a 100 µm nylon mesh. Samples were stained with either (i) CD3, CD8α, CD19b, and NKT or (ii) CD4, CD103, and intracellular FoxP3 according to manufacturer’s protocol (eBiosciences, San Diego, CA). Samples were analyzed using a BD Accuri C6 cytometer and BD FLOW CYTOMETRYDIVA software (Accuri Cytometers Inc., Ann Arbor, MI). The gating strategy is outlined in Supplementary Fig. S1.

Laigaard A., Krych L., Zachariassen L.F., Ellegaard-Jensen L., Nielsen D.S., Hansen A.K, & Hansen C.H. (2020). Dietary prebiotics promote intestinal Prevotella in association with a low-responding phenotype in a murine oxazolone-induced model of atopic dermatitis. Scientific Reports, 10, 21204.

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Multiparametric Flow Cytometry Assay

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Cells were labeled with Abs against CD45, B220, I-A/I-E, CD11c, CD11b, CD8α, CD103, CD80, CD86, PD-L1, H2-D^k, MHC-I^SIINFEKL, Thy1.1, CD25, CD69, IFNγ, and GranzymeB (all obtained from eBioscience, San Diego, CA). For cell surface labeling, 1 × 10⁶ cells were blocked with anti-CD16/CD32 (2.4G2; University of Virginia, Charlottesville, VA) and incubated with the corresponding Abs for 30 min at 4°C in staining buffer (PBS with 2% FBS and 0.1% NaN₃). For cytokine staining, 1 × 10⁶ cells were incubated for 5 hours in IMDM supplemented with 10% FBS, 10 U/mL penicillin G, 2 mM L-glutamine, 5 mM β-mercaptoethanol, and 1μL/mL of GolgiPlug/GolgiStop (BD Biosicences). OT-I cells were restimulated with 2 μg/mL SIINFEKL peptide (AnaSpec). After incubation, the cells were surface labeled as described above, fixed using Cytofix/Cytoperm (BD Biosciences) according to the manufacturer’s instructions prior to intracellular IFNγ staining. All samples were run on a BD FACS Canto II (BD Immunocytometry Systems, San Jose, CA) and analyzed using FlowJo software 8.8.6 (Tree Star Inc., Ashland, OR).

Krueger P.D., Kim T.S., Sung S.S., Braciale T.J, & Hahn Y.S. (2015). Liver-resident CD103+ dendritic cells prime anti-viral CD8+ T cells in situ. Journal of immunology (Baltimore, Md. : 1950), 194(7), 3213-3222.

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Multiparameter Flow Cytometry Analysis

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Cell suspensions were obtained from intestines, spleens, and MLNs and stained in PBS 2% FCS with conjugated antibodies to the following markers: TCR-β (eBioscience), TCR-γδ (eBioscience), CD3 (eBioscience), CD45 (eBioscience), CD4 (eBioscience), CD8a (eBioscience), CD62L (eBioscience), CD44 (BD), CD45R (B220; eBioscience), CD357 (GITR; eBioscience), CCR9 (eBioscience), α4β7 (BioLegend), Foxp3 (eBioscience), IgA (eBioscience), IgM (eBioscience), CD25 (eBioscience), CD103 (eBioscience), CD45RB (BioLegend), and Nrp-1 (R&D Systems). Live/dead cell discrimination was obtained using the Aqua Dead Cell Stain kit (Invitrogen). For cytokine production, cells were stimulated for 4 h with 20 ng/ml PMA (Sigma-Aldrich) and 0.5 µg/ml ionomycin (Sigma-Aldrich). Golgi Stop (1,000×; BD) was added during the last 3 h of stimulation. Cells were fixed and permeabilized using intracellular fixation and permeabilization buffer kit (eBioscience) and stained for conjugated anti–IL-17A (eBioscience) and anti–IFN-γ (eBioscience). Flow cytometry data were acquired at FACSCanto II. Data were analyzed with FlowJo software (version 7.6.5).

Rigoni R., Fontana E., Guglielmetti S., Fosso B., D’Erchia A.M., Maina V., Taverniti V., Castiello M.C., Mantero S., Pacchiana G., Musio S., Pedotti R., Selmi C., Mora J.R., Pesole G., Vezzoni P., Poliani P.L., Grassi F., Villa A, & Cassani B. (2016). Intestinal microbiota sustains inflammation and autoimmunity induced by hypomorphic RAG defects. The Journal of Experimental Medicine, 213(3), 355-375.

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Multicolor Flow Cytometry Phenotyping

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The following antibodies were used for flow cytometry: NK1.1 (PK 136), CD19 (6D5 or 1D3), CD3e (17A2 or 145‐2C11), MHC II(IA/I‐E) (M5/114.15.2), CD11b (M1/70), CD8α (53‐6.7), Ly6C (HK1.4 or AL‐21), CD45.2 (104), B220 (RA3‐6B2), CD45.1 (A20), TCRβ (H57‐597), CD8β (53‐5.8 or YST156.7.7 or eBioH35‐17.2), CD4 (RM4‐5 or GK1.5 or eBioGK1.5), Ter119 (TER‐119), IFN‐γ (XMG 1.2), Siglec H (551), CD11c (N418), IL17A (TC11‐18‐10.1), FoxP3 (FJK‐165), CD103 (M290), Siglec F (E50‐2440), CD64, (X54‐5/7.1), CD135 (A2F10), CD117 (2B8), CD45.2 (104) and XCR1 (ZET) all from eBioscience, BioLegend or BD Biosciences. Antibody to GP2 (2F11 C3) was from MBL.
Flow cytometry was performed according to standard procedures.³⁷ Dead cells identified by addition of propidium iodide (Invitrogen), Viability Dye eFluor®450 (eBioScience), or Red or Aqua LIVE/DEAD Fixable Dead Cell Staining Kit (Life Technologies), and cell aggregates (identified on FSC‐A vs FSC‐W scatterplots) were excluded from analyses. Intracellular staining was performed using the FoxP3 Fixation/Permeabilization Kit (eBioscience) according to manufacturer's instructions. Data were acquired on a FACSAriaII or LSRII (BD Biosciences) and analysed using FlowJo software (Tree Star). Sorting was performed on a FACSAriaII or on a MoFlow®Astrios (Beckman Coulter).

Luda K.M., Da Silva C., Ahmadi F., Mowat A.M., Ohno H., Kotarsky K, & Agace W.W. (2022). Identification and characterization of murine glycoprotein 2‐expressing intestinal dendritic cells. Scandinavian Journal of Immunology, 96(5), e13219.

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Comprehensive Immune Cell Profiling

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Single cells suspensions were stained with fixable Viability Dye eFluor
780 (eBiosciences) in PBS for 10 min at RT. Cells were stained with directly
conjugated Abs in PBS with 0.5% BCS for 20 min at 4°C. Abs purchased from
BioLegend: CD4 (RM4–5), TCRβ (H57–597), CD44 (IM7), CD62L
(MEL-14), CD25 (PC61), ICOS (C398.4A), TIGIT (1G9), CTLA4 (UC10–4B9),
GITR (DTA.1), CD69 (53–7.3). Abs purchased from eBiosciences: KLRG1 (2F1)
and CD103 (2E7). Intracellular stains were performed using a FixPerm Kit
(eBiosciences). Data acquired on an LSR II (BD Biosciences) and analyzed using
FlowJo software (TreeStar).

Sullivan J.M., Höllbacher B, & Campbell D.J. (2018). Dynamic expression of Id3 defines the stepwise differentiation of tissue-resident regulatory T cells. Journal of immunology (Baltimore, Md. : 1950), 202(1), 31-36.

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Multicolor Flow Cytometry Analysis

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All used at 1:200: CD11b (Clone M1/70, Biolegend, Cat 10137), CD45 (Clone 30-F11, eBioscience, Cat 56-0451-82), MHCII (Clone M5/114.15.2, Biolegend, Cat 107626), CD86 (Clone GL1, BD Biosciences, 553691), CD80 (Clone 16-10A1, Biolegend, 104706), CD11c (Clone N418, Biolegend, Cat 117335), CD103 (Clone 2E7, eBioscience, Cat 48-1031-80), CD8a (Clone 53-6.7, Biolegend, Cat 100712), CD4 (Clone RM4-5, BD Biosciences, Cat 550954), CD69 (Clone H1.2F3, eBiosciene, Cat 12-0691-81), Ly6C (Clone HK1.4, Biolegend, Cat 128003), Ly6G (Clone 1A8, Biolegend, Cat 127616), CD160 (Clone 7H1, Biolegend, Cat 143007), Rat-anti-P2RY12 (Clone S16007D, Biolegend, Cat 848002), and Goat-anti-Rat-AlexaFluor 350 (Invitrogen, Cat A21093). WNV-specific CD8+ T cells were identified with fluorescent-labeled immunodominant D^b-restricted NS4B peptide.

Funk K.E, & Klein R.S. (2019). CSF1R antagonism limits local restimulation of antiviral CD8+ T cells during viral encephalitis. Journal of Neuroinflammation, 16, 22.

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