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3000 dalton cutoff centrifuge tube

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The 3000 Dalton cutoff centrifuge tube is a laboratory equipment designed to separate and isolate molecules and particles based on their molecular weight. The tube has a specific molecular weight cutoff of 3000 Daltons, which means it can effectively separate molecules with a molecular weight above or below this threshold during the centrifugation process.

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Centrifuge tube (6 citations) Dalton (1 citations)

2 protocols using 3000 dalton cutoff centrifuge tube

1

Murine Astrocyte Isolation and Conditioned Media

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Murine cortical primary astrocytes were cultured from postnatal day 1-3 pups as described (51 (link),52 (link)). Astrocyte-enriched glial cell cultures were checked for purity and only preparations with >90% GFAP+ cells were used. To obtain 10X concentrated astrocytic-conditioned media (CM), 100% confluent astrocyte cultures were rinsed and incubated for 72 h with serum-free media containing different hormonal treatments. Supernatants were collected and cleared from cell debris and then concentrated by passing it through a 3000 Dalton cutoff centrifuge tube (Millipore) until volume was 1/10 of the initial volume. CM was used immediately or stored at −80C.
Sartorius C.A., Hanna C.T., Gril B., Cruz H., Serkova N.J., Huber K.M., Kabos P., Schedin T.B., Borges V.F., Steeg P.S, & Cittelly D.M. (2015). Estrogen promotes the brain metastatic colonization of triple negative breast cancer cells via an astrocyte-mediated paracrine mechanism. Oncogene, 35(22), 2881-2892.
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2

Murine Astrocyte Isolation and Conditioned Media

Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine cortical primary astrocytes were cultured from postnatal day 1-3 pups as described (51 (link),52 (link)). Astrocyte-enriched glial cell cultures were checked for purity and only preparations with >90% GFAP+ cells were used. To obtain 10X concentrated astrocytic-conditioned media (CM), 100% confluent astrocyte cultures were rinsed and incubated for 72 h with serum-free media containing different hormonal treatments. Supernatants were collected and cleared from cell debris and then concentrated by passing it through a 3000 Dalton cutoff centrifuge tube (Millipore) until volume was 1/10 of the initial volume. CM was used immediately or stored at −80C.
Sartorius C.A., Hanna C.T., Gril B., Cruz H., Serkova N.J., Huber K.M., Kabos P., Schedin T.B., Borges V.F., Steeg P.S, & Cittelly D.M. (2015). Estrogen promotes the brain metastatic colonization of triple negative breast cancer cells via an astrocyte-mediated paracrine mechanism. Oncogene, 35(22), 2881-2892.
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