Sgc 7901

GC tissues were obtained from GC patients treated at the Affiliated People's Hospital of Jiangsu University (Zhenjiang, Jiangsu, China). The study protocol was approved by the Ethics Committee of Jiangsu University. Informed consent forms were obtained from all subjects. GCMSCs were isolated from human GC tissues as previously described 25 (link). Briefly, fresh GC tissues were cut into approximately 1 mm³-sized pieces, which were then adhered to 60 mm cell culture dishes (Corning, USA) and were cultured in MEM-ALPHA (Biological Industries, Israel) supplemented with 10% fetal bovine serum (FBS, Biological Industries) at 37°C with 5% CO_2. The culture medium was replaced every 3 days. When the fibroblast-like cells reached 85% confluence, the cells were trypsinized and expanded for up to five passages.
The human GC cell lines SGC-7901, MGC-803, HGC-27, and AGS were obtained from the Chinese Academy of Sciences Type Culture Collection Committee Cell Bank (Shanghai, China). SGC-7901, MGC-803, and HGC-27 were cultured in RPMI-1640 (Biological Industries) with 10% FBS. AGS were cultured in DMEM/F-12 (Biological Industries) with 10% FBS at 37°C with 5% CO_2.

Human gastric epithelial cell line GES-1 was purchased from Gefan Biological Technology (Shanghai, China). Human GC cell lines MGC-803, AGS, SGC-7901 and HGC-27 were bought from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All cell lines were regularly tested for Mycoplasma. MGC-803 cells were maintained in high-glucose DMEM (Gibco, USA). AGS cells were cultured in DMEM/F-12 medium (Bioind, Israel). SGC-7901 and HGC-27 cells were propagated in RPMI-1640 (Bioind, Israel). The cell-culture medium was supplemented with 10% fetal bovine serum (FBS; Bovogen, Australia) before use. All cell lines were cultured at 37 °C with a 5% CO₂ atmosphere following the standard protocol.