To discard the possibility that the extract possesses the non-specific muscle relaxant or sedative effect, which might result in the false positive interpretation of the antinociceptive activity of MEDL, the mice receiving MEDL was also subjected to the rotarod test as described [14 (link)]. The apparatus consisted of a horizontal bar with a diameter of 3 cm and subdivided into five compartments (Ugo Basile, model 47600). Twenty-four hours before the experiment, the untreated mice underwent the selection processes wherein each of them was trained on the apparatus by placing them on the rotarod at a fixed speed of 20 rpm and those that were able to remain on the apparatus for 120 sec without falling were selected. The selected mice (n = 6) were treated (p.o.) with vehicle (2% DMSO), diazepam (DZP; 4 mg/kg; standard drug) or MEDL (300 mg/kg, p.o.) 60 min before being subjected to the test. The latency took to remain on the apparatus before falling was recorded using a chronometer for 120 s at 5, 10, and 15 min. The average time the mice took to stay on the rotarod equipment for each group was expressed as a result.
Model 47600
Manufactured by Ugo Basile
Sourced in Italy
The Model 47600 is a versatile instrument designed for the assessment of thermal nociception in rodents. It utilizes a thermal stimulation method to measure the latency of paw withdrawal response, providing an objective measure of thermal sensitivity.
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Lab products found in correlation
24 protocols using model 47600
1
Assessing Non-Specific Muscle Relaxation of MEDL
2
Neuroprotective AAV9 Treatment in Mice
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Mice were fed standard chow (LabDiet 5013) and maintained at 22 ± 2°C with a humidity of 50% ± 10% and a 14 h light/10 h dark cycle. Pregnant C57BL/6 females at gestational day 17 were obtained from Charles River, housed in individual cages and closely monitored until delivery. Between 18 and 24h after birth, pups of either sex were randomly assigned to the Nudt7cyt or GFP group and injected through the superficial temporal vein with 3.0*1011 genome copies of the respective scAAV9 in PBS (100 μl) containing 0.001% pluronic F-68 [33 (link)]. The grip strength of the front limbs was measured using a grip-strength meter (Ugo Basile, model 47200). The rotarod test was conducted using an accelerating rotarod (Ugo Basile, model 47600). The mice were trained over 2 consecutive days by placing them on the drum rotating at 4, 8, 15, 24, 30 and 40 rpm for 60 sec, allowing 5 min rest before each increase in speed. During the following 3 days the mice were tested for a maximum of 300 seconds at each speed, with 5 min breaks in between. For each mouse, the time spent on the rotarod before falling at a particular speed was recorded and averaged over the 3 days. For all the experiments, the number of mice/group is indicated in the correspondent figure or figure legend.
3
Accelerated Rotarod Test for Mice
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Mice were tested by a tester blinded to the treatment groups on an accelerated rotarod apparatus (model 47600; Ugo Basile, Varese, Italy). For distribution to groups of equal abilities at baseline, mice were first tested at 5 weeks of age prior to treatment. Mice were habituated to the rotarod for 4 minutes, then subjected to three trials per day (with at least 30 minutes of rest between trials) for 4 consecutive days. For each trial, acceleration was from 4 to 40rpm over 5 minutes, and then speed was maintained at 40rpm. Latency to fall (or if mice hung on for 2 consecutive rotations without running) was recorded for each mouse per trial. Trials were stopped at 500 seconds, and mice remaining on the rod at that time were scored as 500 seconds. Two‐way analysis of variance followed by a Tukey post hoc analysis was used to assess for significant differences. Variables were time and treatment.
4
Accelerated Rotarod Assay for Mice
5
Assessing Motor Coordination in Rodents
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The Rotarod is a motor-coordination test, which consists of a rotating drum (Ugo Basile, model 47600, Comerio, Italy), in which animals are placed, while the drum is accelerating from 4 to 40 rpm. Maximal velocity is achieved after 260 s (maximum testing time 300 s). The time until each animal drops off the drum is measured [23 (link), 24 (link)]. Animals were trained three times each on three consecutive days before MCAO. Following a baseline evaluation, animals were tested at 3 days, 7 days and then weekly until 56 day post-MCAO.
6
Assessing Motor Coordination with RotaRod
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The RotaRod is a rotating drum with a speed accelerating from 6 to 40 rpm (model 47600; Ugo Basile, Comerio, Italy), which allows to assess motor coordination skills (Kilic et al., 2010 (link)). Maximum speed is reached after 245 s, and the time at which the animal drops off the drum is evaluated (maximum testing time: 300 s). Measurements were performed five times each on the same occasion when grip strength was evaluated. For all five measurements, mean values were computed, from which percentage values (post-ischemic vs. pre-ischemic) were calculated. Pre-ischemic data did not differ between groups.
7
Rotarod Performance Evaluation
8
Rotarod Test for Motor Coordination
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Motor co-ordination was assessed by using a rotarod test (Model 47600, Ugo Basile, Varese, Italy). During the training sessions, mice were placed on a rotating drum set to accelerate from 4 to 80 rpm over a 5-min period for three trials with 30-min intervals every day for 3 consecutive days. During the testing sessions, mice were given three trials on the rotarod apparatus set to accelerate from 4 to 80 rpm with a maximum time of 300 s and 30 min intertrial rest interval at Day 2, 4 and 6 post-injury. The latency to fall was recorded as a measure of motor function.
9
Rotarod Motor Coordination Test Protocol
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The Rotarod is a motor coordination test, which consists of a rotating drum (Ugo Basile, model 47,600, Comerio, Italy), in which animals are placed while the drum is accelerating. The time until each animal drops off the drum is measured (maximum testing time 300 s) [16 , 17 ]. Animals were trained three times each on three consecutive days before MCAO. Following a baseline evaluation, animals were tested weekly until 42 dpi, as shown in Fig. 1 . The test was performed three times at each time point. Means were calculated for each time point. Our group has previously used this protocol for evaluating the efficacy of restorative stroke therapies [16 , 17 ].
10
Rota-Rod Assay for Sedative Effect
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The method used was conducted similarly to the one that was described previously [11 (link)]. The apparatus consisted of a horizontal bar with a diameter of 3 cm, and subdivided into five compartments (UgoBasile, model 47600). The animals were placed on the rota–rod at a fixed speed of 20 revolutions per min. Those that were able to remain on the apparatus for 120 s without falling were selected 24 h before test. The mice (n = 6) were treated with the vehicle (10% DMSO, 10 mL/kg, p.o.), DZP (4 mg/kg, p.o., positive control), or PECN (500 mg/kg, p.o.) 60 min before the test. The average time the mice spent to stay on the rota–rod was measured for 120 s at 5, 10, and 15 min after the administration of PECN, respectively.
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