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Tcldm9

Manufactured by Thorlabs
Sourced in United States

The TCLDM9 is a thermoelectric cooler module that is designed to control the temperature of various optical and electronic components. It features a compact and rugged design, making it suitable for integration into a wide range of applications.

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9 protocols using tcldm9

1

Characterization of Synthesized Phosphor Powder

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The crystal structure of the synthesized phosphor powder was measured using an X-ray diffraction apparatus (XRD, X'Pert PRO MPD, 40 kV, 30 mA) having Cu-Kα radiation (wavelength: 1.5406 Å) at a scan rate of 4° min−1 at a diffraction angle of 10° to 70°. The size and microscopic surface shape of the crystal grains were photographed with a field emission scanning electron microscope (FE-SEM, CZ, MIRA I LMH, TESCAN). To measure the fluorescence spectrum by up-conversion, a semiconductor pulse laser (TCLDM9, Thorlabs) that emitted an output of 200 mW at a wavelength of 980 nm as excitation, and a spectrometer (HR4000, Ocean Optics) with a photomultiplier connected were used to measure the emission spectrum. To analyze the fluorescence mechanism by up-conversion, the energy absorption and energy transfer processes in the excited state were analyzed by changing the intensity of the pulsed laser and measuring the changes in the intensity of the fluorescence.
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2

Phototriggered Liposomal Delivery in Tumor Studies

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To examine phototriggering of liposomes in our animal studies, a 658 nm nominal wavelength laser diode was used. When the tumors reached a volume of 200–300 mm3, 200 μL of either Formulation IV (with calcein and HPPH) liposomes or Formulation V (with calcein only) liposomes were injected into the tail vein. Typical amounts of HPPH and lipid in 0.2 mL of liposomes corresponded to 30 μg HPPH and 0.6 mg of lipid, corresponding to 4×1012 liposomes (for calculations, see Supplementary material). Four hours after injection, the mice were anesthetized with isoflurane and one tumor per mouse was treated for 5 minutes with a cw-diode laser (Thorlabs, TCLDM9) emitting 90 mW (spot size 0.9 cm Ø) at a wavelength of 658 nm. The second tumor was left untreated as a reference. The mice in the biodistribution group were left untreated.
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3

Compact Continuous-Wave Photoacoustic Tomography

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Figure S2b shows the schematic drawing of the CW-PAT system, utilizing an inexpensive, compact, and durable laser diode (L808P1000MM, Thorlabs, maximum power: 1000 mW; wavelength: 808 nm) as the excitation source. The laser diode was installed in a laser mount (TCLDM9, Thorlabs), which was driven by a laser diode current controller (LDC240C, Thorlabs). We modulate the power of the laser diode at 1 MHz with 100% modulation depth by applying an 8.5 V square wave to the RF input of laser mount. In front of the laser diode, we used a convex lens (C230TMD-B, Thorlabs) to collimate the diode output. Similar to the pulsed PA experiment, we sealed the contrast in a tube, which was covered by a slice of 3-mm thick breast tissue. Position of the sample was adjusted to ensure that the tube phantom could generate the highest PA signal. The light intensity on the surface of chicken tissue was quantified to be 500 mW/cm2, which is within the ANSI safety limit of 808 nm (3.29 W/cm2). A 1 MHz single element transducer (E1012-SU, Mana) with a 12.7 mm diameter is co-axially placed beneath the laser beam to detect the generated PA signal, which was first amplified 80 dB by a low noise amplifier (351A-3-50-NI, Analog Modules) and then acquired by an oscilloscope (Tektronix DP3034).
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4

Laser-Based Temperature Monitoring of PMMA Solutions

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In a poly(methyl methacrylate) (PMMA) cuvette (with a 4.5 mm window width and 12.5 mm depth), 300 μL of solution was illuminated by an 805 nm temperature controlled laser diode with 500 mW output (TCLDM9, Thorlabs). The solution temperature was measured every minute with an immobilized FLIR E6 thermal imager (FLIR Systems, Inc.) from above to avoid obstruction by the cuvette walls. A sample of phosphate-buffered saline (PBS), pH 7.4, was illuminated and used as a baseline.
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5

Electronically Synchronized Optical Pulses

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To generate optical pulses with electronically defined time delay, we first generated electronic pulses synchronized to the femtosecond laser. The femtosecond laser output (PHAROS, Light Conversion) had a repetition rate of 150 kHz defined by a regenerative amplifier, whose internal clock was used to trigger an electronic pulse generator (8082A, Hewlett-Packard). The output electronic pulse had a pulse duration of ~3 ns, which was then converted to optical pulses by an rf-coupled laser diode module (TCLDM9, Thorlabs). The relative time delay between the femtosecond laser output and the diode laser output could thereby be accurately controlled with the electronic delay in the pulse generator. The pulse duration of the nanosecond optical pulse was characterized by a fast photodiode and oscilloscope, from which we determined a pulse width of ~3 ns.
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6

Optical Characterization of Nanorings

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The samples were prepared by dropcasting a diluted solution of nanorings (dilution up to 5000 times) onto ~ 5 x 5 mm glass slides. They were then mounted on the cold finger of a cryostat (Oxford Instrument) allowing the control of the temperature from ≈ 4 K to room temperature. The samples were excited with a continuous wave diode-pumped solid state laser (Thorlabs DJ532) emitting at 533 nm, mounted in its temperature controlled laser mount (Thorlabs TCLDM9). The excitation was focused using a microscope objective (NA = 0.6, spot size ≈ 1 μm). The incident power density was kept around 5 μW/μm². The luminescence was collected using the same optic and spectrally analyzed with an ACTON SP2760i Roper Scientific-Princeton Instruments spectrometer coupled to a nitrogen-cooled SPEC10-2KB-LN (RS-PI) CCD camera (1200 lines per mm grating).
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7

Laser-based Visual Stimulation for Retinal Implants

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A Large Spot slit-lamp adapter (Iridex Corp., Mountain View, CA, USA) was used to project a 3-mm-diameter laser through a slit-lamp (Haag-Streit, Mason, OH, USA) into the eye. Two laser diodes (DJ532-40 and L852P150; Thorlabs, Newton, NJ, USA) were used to emit 10-ms pulses every 500 ms of either 532-nm 100-μW/mm2 green light or 852-nm 3.4-mW/mm2 infrared light. Green 532-nm light was used as a positive control to verify natural VEPs were elicited from retina over the implant. Although the device is sensitive to both visible and IR light, we used 852-nm IR light to activate the device to avoid natural stimulation of the rabbit photoreceptors. The laser diodes were mounted in a temperature-controlled mount (TCLDM9; Thorlabs) and driven by a Benchtop Laser Controller (ITC4020; Thorlabs). In patients, a glasses-mounted camera will capture the visual scene and project patterns of IR light into the implanted eye via a DLP pico display (Texas Instruments, Dallas, TX, USA) while still permitting visible light to pass through allowing for residual natural vision.
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8

Characterization of Rare-Earth Phosphor Powder

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TThe crystal structure of the synthesized phosphor powder was determined using an X-ray diffraction apparatus (XRD, X’Pert PRO MPD, 40 kV, 30mA) with CuKα radiation (wavelength: 1.5406 Å) at a scan rate of 4°/min and a diffraction angle of 10 to 70°. Field-emission scanning electron microscopy (FESEM) was used to characterize the size, microscopic surface, and shape of the crystal grains (FESEM, Brono, CZ, MIRA I LMH, TESCAN). A semicon-ductor pulse laser (TCLDM9, Thorlabs, Jessup, MD, USA) with an emission output of 100 mW at an excitation wavelength of 980 nm and a spectrometer (HR4000, Ocean Optics, Ostfildern, Germany) connected with a photomultiplier was used to measure the fluores-cence spectrum by UC and the emission spectrum. Raman spectroscopy (JP/NRS-3300, 532 nm, 100 mW solid-state primary laser) was mainly employed to understand the fluorescence mechanism of UC. The energy absorption and energy transfer processes in the excited state were analyzed by varying the intensity of the pulsed laser and meas-uring the changes in the fluorescence intensity using Raman spectroscopy.
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9

Chiral Photocurrent Characterization in IGZO/Au Nanoparticle Photodetector

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Photocurrent at IGZO/chiral gold nanoparticle photodetector was measured when the device was illuminated with CPL at normal incident angle. Photocurrent was calculated by subtracting current measured without light illumination from that with light illumination. The output curve and transfer curve were inspected using semiconductor characterization system (Keithley SCS 4200). The linearly polarized light was created using temperature-controlled laser diode mount (Thorlabs, TCLDM9), and lasing wavelength is tailored by mounting different laser diodes having characteristic wavelength at 635 nm (Thorlabs, HL5322G) and 780 nm (Thorlabs, L780P010). Circularly polarized light (CPL) was precisely generated with a manually aligned quarter-wave plate (Thorlabs, AQWP05M-600), and ideality of circular polarization was confirmed by commercial polarimeter (Thorlabs, PAN5710VIS). The power of incident light was measured by power meter (Newport, 1918-R), and the intensity of LCP and RCP light is precisely calibrated to calculate photoresponsivity.
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