Il 17a mice

Rag2−/− mice (008449), Tcrδ−/− mice (002120), muMT−/− mice (002288), Tcrα−/− mice (002116), Il17a−/− mice (016879) were from Jackson Lab. Il17f−/− mice were from Dr. Sarah L. Gaffen and Dr.Yoichiro Iwakura’s lab, Il17rc−/− and Il17ra−/− were from Amgen, Il17RC^flox/flox mice were from Dr. Jay K. Kolls’ lab³¹ . Vγ6Vδ1 Tg mice were from Dr. Diane Mathis’ lab. Adiponectin Cre mice (028020) and UCP1 Cre mice (024670) were from Jackson lab³² . VGLUT3-ires-Cre mice were from Dr. Bradford Lowell’s lab³³ . TGFβ antibody (Bioxcell # BE0057 Clone 1D11.16.8) and Isotype control antibody (Bioxcell # BE0083) were i.p. injected to WT B6 mice for 3 weeks at 10mg/kg 3 times a week. sb431542 was i.p. injected to WT B6 mice at 4.2mg/kg/day for 3 weeks. All animal studies were approved by the Institutional Animal Care and Use Committee of Beth Israel Deaconess Medical Center.

All animal procedures were approved by the Animal Care and Use Committee of Indiana State University, the host campus of IUSM-Terre Haute. BALB/c or C57BL6/J mice were obtained from Envigo or Jackson Laboratory, IL-17A−/− mice were obtained from Dr. David Wilkes, TCRδ−/− mice aged 5 weeks were obtained from Jackson Laboratory. Mice were allowed to rest 1–4 weeks prior to experiments. A subset of mice were bred at the IUSM-Terre Haute animal facility with offspring used in subsequent experiments at 7–10 weeks of age. For repeated aspiration, suspensions of 2 × 10⁶ conidia were delivered involuntarily as previously described [25 (link)]. After two weeks of twice weekly aspiration, mice were rested for two weeks, when a final aspiration was administered. Mice were euthanized 72 hrs after the final challenge with sodium pentobarbitol, and lungs were perfused with 10 ml phosphate buffered saline (PBS). Bronchoalveolar lavage fluid (BALF) was collected from the perfused lungs as previously described [26 (link)].