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2 protocols using mouse anti ku70

1

Antibody Reagents for Protein Analysis

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The following first antibodies were purchased: rabbit anti-HNRNPU (#A3917), rabbit anti-PUF60 (#A6709), rabbit anti-YBX1(#A7704), rabbit anti-DDX9 (#A4563), rabbit anti-NCL (#A5904), rabbit anti-ILF3 (#A8186), rabbit anti-DKC1(#A1862), rabbit anti-TOP2A (#A16440), rabbit anti-RPA70 (#A0990) and rabbit anti-STAU1 (#A4131) from Abclonal (Woburn, MA); mouse anti-Ku70 (#SC-17789) from Santa Cruz (Dallas, TX); rabbit anti-Ku80 (#2180S) and rabbit anti-His (#12698S) from Cell Signaling; mouse anti-Flag (#200-301-B13) from Rockland (Limerick, PA); mouse anti-β-actin (#A5441) from MilliporeSigma; anti-GST (#SC-138) from Santa Cruz (Dallas, TX); rabbit anti-Strep-tag II (#ab183907) from Abcam (Waltham, MA);
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2

Antibody Production and Validation

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Rat anti-HBoV1 NS1C antibody was produced previously (108 (link)). The following primary antibodies were purchased: mouse anti-Flag (number 200-301-B13) from Rockland (Limerick, PA); rabbit anti-Strep-tag II (number ab183907) from Abcam (Waltham, MA); mouse anti-β-actin (number A5441) from MilliporeSigma; mouse anti-Ku70 (number SC-17789) and mouse anti-GST (number SC-138) from Santa Cruz (Dallas, TX); rabbit anti-Ku70 (number A7330), rabbit anti-HSPA8 (number A14001), rabbit anti-GTF2F1 (number A2489), rabbit anti-ORC3 (number A15415), rabbit anti-DNA-PKcs (number A1419), rabbit anti-DHX9 (number A4563), rabbit anti-SNW1 (number A14580), rabbit anti-PABPC1 (number A14872), rabbit anti-DDX17 (number A17078), rabbit anti-RPA1 (number A0990), and rabbit anti-MCM3 (number A1060) from Abclonal (Woburn, MA); rabbit anti-SFPQ (number A301-321A-T) from Bethyl (Montgomery, TX); rabbit anti-HSPA1b (number A59434-020) from EpiGentek (Farmingdale, NY); rat anti-HSPA8 (number NBP1-97868) from Novus Bio (Centennial, CO); anti-BrdU (clone B44) from BD Biosciences (San Jose, CA); and anti-GAPDH (number 2118S) from Cell Signaling (Danvers, MA).
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