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Km12c

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The KM12C is a type of cell line used in biological research. It is a colorectal adenocarcinoma cell line derived from a human colon cancer sample. The KM12C cell line is commonly used in studies related to cancer biology and drug development.

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Lab products found in correlation

Ht 29, by Korean Cell Line Bank (6 mentions) Hct116, by Korean Cell Line Bank (5 mentions) Sw480, by Korean Cell Line Bank (5 mentions) Dld 1, by Korean Cell Line Bank (5 mentions) Sw620, by Korean Cell Line Bank (5 mentions) Km12sm, by Korean Cell Line Bank (5 mentions) Km12l4, by Korean Cell Line Bank (3 mentions) Sw1116, by Korean Cell Line Bank (3 mentions) Ls174t, by Korean Cell Line Bank (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Minimum essential medium (mem), by Welgene (2 mentions) Hct 15, by Korean Cell Line Bank (2 mentions) Rpmi 1640, by Welgene (2 mentions) Dulbecco modified eagle medium (dmem), by Welgene (2 mentions) Colo205, by Korean Cell Line Bank (2 mentions) Caco 2, by Korean Cell Line Bank (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Recombinant human trail, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) L15 medium, by Welgene (1 mentions)

7 protocols using km12c

1

Colorectal Cancer Cell Lines Cultivation

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Four human colorectal cancer cell lines, HCT116, KM12C, HT29, and SW480, were purchased from the Korean Cell Line Bank (Korean Cell Line Research Foundation, Seoul, Korea) and maintained in RPMI1640 supplemented with 10% fetal bovine serum (Gibco BRL/Life Technologies, Inc., Gaithersburg, MD, USA). They were cultured at 37 °C in a humidified chamber containing 5% CO2. Human recombinant TRAIL purchased from Sigma were used at indicated concentrations.
Lee W.H., Han M.W., Kim S.H., Seong D., An J.H., Chang H.W., Kim S.Y., Kim S.W, & Lee J.C. (2020). Tristetraprolin Posttranscriptionally Downregulates TRAIL Death Receptors. Cells, 9(8), 1851.
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2

Colon Cancer Cell Line Culture

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Ten colon cancer cell lines (DLD1, HT29, HCT116, HCT15, SW620, LOVO, SW480, KM12C, KM12L4, and KM12SM) were obtained from the Korean Cell Line Bank (Seoul, Korea). The cells were cultured in MEM, DMEM, RPMI1640, or L15 medium (Welgene, Daegu, Korea) supplemented with 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Gibco), and maintained in a humidified incubator with 5% CO2 at 37 °C.
Jang B.G., Kim H.S., Bae J.M., Kim W.H., Hyun C.L, & Kang G.H. (2020). Expression Profile and Prognostic Significance of EPHB3 in Colorectal Cancer. Biomolecules, 10(4), 602.
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3

Comprehensive CRC Cell Line Characterization

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Forty-eight human CRC cell lines (SNU-61, SNU-70, SNU-81, SNU-175, SNU-254, SNU-283, SNU-407, SNU-479, SNU-503, SNU-769A, SNU-769B, SNU-796, SNU-977, SNU-1033, SNU-1040, SNU-1047, SNU-1181, SNU-1197A, SNU-1235, SNU-1411, SNU-1406, SNU-1544, SNU-1684, SNU-1746, SNU-C1, SNU-C2A, SNU-C4, SNU-C5, Caco-2, COLO201, COLO205, COLO320, DLD1, HCT-8, HCT-15, HCT-116, HT-29, LoVo, LS174T, NCI-H716, SW-403, SW-480, SW-620, SW-1116, WiDr, KM12C, KM12SM, and KM12L4) were obtained from the Korean Cell Line Bank. All cell lines were maintained in RPMI1640 media with 10% fetal bovine serum at 37°C and 5% CO2. All cell lines were tested and authenticated by Korean Cell Line Bank using DNA finger printing analysis.
Kim S.C., Hong C.W., Jang S.G., Kim Y.A., Yoo B.C., Shin Y.K., Jeong S.Y., Ku J.L, & Park J.G. (2018). Establishment and Characterization of Paired Primary and Peritoneal Seeding Human Colorectal Cancer Cell Lines: Identification of Genes That Mediate Metastatic Potential. Translational Oncology, 11(5), 1232-1243.
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4

Culturing Colorectal Cancer Cell Lines

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The colorectal cancer cell lines HCT-116, SW1116, LS174T, HT-29, KM12C, KM12SM, SW48, SW480, SW620, DLD-1, LOVO, COLO205 were obtained from the Korean Cell Line Bank (Seoul, Korea) and were maintained in DMEM (Gibco-BRL, Grand Island, NY) supplemented with 10% fetal bovine serum and 100 μg/ml antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin. Cells were maintained at 37°C in a humidified, 5% CO2/air atmosphere.
Park S.Y., Lee S.J., Cho H.J., Kim T.W., Kim J.T., Kim J.W., Lee C.H., Kim B.Y., Yeom Y.I., Lim J.S., Lee Y, & Lee H.G. (2016). Dehydropeptidase 1 promotes metastasis through regulation of E-cadherin expression in colon cancer. Oncotarget, 7(8), 9501-9512.
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5

Colon Cancer Cell Lines Cultivation

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Colon cancer cell lines SW480 (KCLB-10228; colon adenocarcinoma), SW620 (KCLB-10227; colorectal carcinoma), HT-29 (KCLB-30038; rectosigmoid colon adenocarcinoma), DLD1 (KCLB-10221; colon adenocarcinoma), Caco2 (KCLB-30037.1; colon adenocarcinoma), LoVo (KCLB-10229; colon adenocarcinoma, KM12C (KCLB-80015; colon carcinoma), and KM12SM (KCLB-80016; colon carcinoma) cells were purchased from the Korean Cell Line Bank (Seoul, Korea). RKO (ATCC CRL-2577; colon carcinoma), HCT116 (ATCC CCL-247; colorectal carcinoma), and LS174T (ATCC CL-188; colorectal adenocarcinoma) cells were purchased from the American Type Culture Collection (ATCC, USA). Each cell line was authenticated through STR profiling from the cell line bank. Cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS) (HyClone) and 100 µg/ml antibiotics [100 U/ml penicillin and 100 µg/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.)] at 37°C under an atmosphere of 5% CO2 in a humidified incubator.
Park S.Y., Kim J.T., Park E.S., Hwang Y.S., Yoon H.R., Baek K.E., Jung H., Yoon S.R., Kim B.Y., Cho H.J, & Lee H.G. (2021). Collapsin response mediator protein 4 enhances the radiosensitivity of colon cancer cells through calcium-mediated cell signaling. Oncology Reports, 45(4), 6.
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6

Culturing of Human Cell Lines for Experiments

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The human CRC cell lines KM12C was obtained from the Korean Cell Line Bank (catalog no.: 80015) (16 , 17 (link)). The cells were cultured as recommended in a complete medium consisting of Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, and 100 U/ml penicillin-streptomycin (all from Invitrogen-Thermo Fisher Scientific, Waltham, MA, USA). The human fibroblast cell lines MRC-5, derived from fetal lung tissue, and WS1, derived from fetal midscapular skin, were obtained from the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan) and were cultured as recommended in the Minimum Essential Medium (MEM) with 10% FBS, 2 mM L-glutamine, and 100 U/ml penicillin-streptomycin (all from Invitrogen). Conditioned medium (CM) was collected from the cell culture after 48 hours of culturing. All cell lines were authenticated using a short-tandem repeat profiling.
Peng K.Y., Jiang S.S., Lee Y.W., Tsai F.Y., Chang C.C., Chen L.T, & Yen B.L. (2021). Stromal Galectin-1 Promotes Colorectal Cancer Cancer-Initiating Cell Features and Disease Dissemination Through SOX9 and β-Catenin: Development of Niche-Based Biomarkers. Frontiers in Oncology, 11, 716055.
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7

Colorectal Cancer Cell Line Cultivation

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Twelve CRC cell lines (DLD1, HT29, SW620, HCT116, LoVo, KM12C, KM12SM, KM12L4, HCT116, LS174T, SW1116, and Colo205) were purchased from the Korean Cell Line Bank (https://cellbank.snu.ac.kr, Seoul, Korea). Cells were cultured in RPMI1640, MEM, DMEM, or L15 medium (Welgene, Daegu, Korea) at 37 °C in a humidified incubator with 5% CO2 according to the guidelines. All culture media contained 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA) and 1% penicillin/streptomycin (Gibco).
Jang B.G., Kim H.S., Bae J.M., Kim W.H., Kim H.U, & Kang G.H. (2020). SMOC2, an intestinal stem cell marker, is an independent prognostic marker associated with better survival in colorectal cancers. Scientific Reports, 10, 14591.
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