ΔΨm was determined using a JC-1 dye. The cells were seeded in 24-well plates at a density of 5 × 104 cells/well. To assess the integrity of ΔΨm, the cells were pre-treated with OLE and RUT in different concentrations (1, 10, 50 μM) for 6 h, followed by 100 μM of 6-OHDA treatment for 8 h and then stained with 10 μM JC-1 at 37 °C for 30 min in darkness. Then the cells were washed twice with PBS. The JC-1 fluorescence images were captured and processed using Leica LAS AF7000 software. Images were analyzed in terms of fluorescence integrities and the ratios of red (non- apoptotic cells)/green (apoptotic cells) were calculated by Image J Software (NIH Image, USA) compared with control and 6-OHDA treated cells.
Las af7000
Manufactured by Leica
The LAS AF7000 software is an imaging platform developed by Leica for advanced microscopy applications. The software provides a comprehensive suite of tools for image acquisition, processing, and analysis. It supports a wide range of Leica microscopes and imaging devices, allowing users to capture and manage high-quality digital images. The LAS AF7000 software is designed to deliver efficient and streamlined workflows for researchers and scientists working in various fields, such as life sciences and materials science.
Automatically generated - may contain errors
2 protocols using las af7000
1
Mitochondrial Membrane Potential Assay
2
Paraffin-Embedded Gynoecia Sectioning and Staining
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Tissues were fixed for 16 h at 25°C in 3.7% formaldehyde, 5% acetic acid, and 50% ethanol and subsequently dehydrated through an ethanol series until 70%. The tissues were embedded in paraffin. An RM 2125 rotary microtome (Leica) was used to make 10 µm transverse sections of Col, spt-12 and hat3 athb4 gynoecia at stage 12. Paraffin was removed from the sections by two rounds of incubation in 100% Histoclear (National Diagnostics) for 10 min at room temperature, followed by two washes in 100% ethanol for 2 min at room temperature, air-dried for 30 min and stained for 10 min by an aqueous solution containing 0.005% Toluidine blue O (Acros Organics). Slides were washed for 1 min in water; sections were mounted in a histological mounting medium Histomount (National Diagnostic) and examined under Leica DM600 light microscopy. Images were taken using the Leica LAS AF7000 software.
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