Gapdh antibody

Manufactured by Fujifilm

Sourced in United States, Japan

The GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) antibody is a laboratory reagent used in various research applications. GAPDH is a ubiquitous enzyme involved in the glycolytic pathway, making it a common loading control or reference protein in experiments. The GAPDH antibody can be used to detect and quantify the GAPDH protein in biological samples through techniques such as Western blotting, immunohistochemistry, and immunoprecipitation.

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Lab products found in correlation

Anti α sma antibody, by Agilent Technologies (1 mentions) Anti mouse igg horseradish peroxidase, by Cell Signaling Technology (1 mentions) Ecl prime reagent, by GE Healthcare (1 mentions) Pab240, by Abcam (1 mentions) Horseradish peroxidase hrp conjugated goat anti rabbit and anti mouse igg antibodies, by Jackson ImmunoResearch (1 mentions) Iblot 2 dry blotting system, by Thermo Fisher Scientific (1 mentions) Imagequant las 500, by GE Healthcare (1 mentions) Ab28364, by Abcam (1 mentions)

Close spelling variants of this product

GAPDH (29 citations) Anti-GAPDH antibody (8 citations) Anti-GAPDH monoclonal antibody (4 citations) HRP-conjugated anti-GAPDH antibody (2 citations)

4 protocols using gapdh antibody

Monocrotaline-induced Pulmonary Hypertension

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Reagent sources: Monocrotaline (Wako, Osaka, Japan) and recombinant mouse canstatin (produced by our laboratory [8 (link)]).
Antibody sources: anti-α-SMA antibody (Dako, Glostrup, Denmark), anti-canstatin antibody (Boster Biological Technology, Pleasanton, CA, USA), anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (Wako), anti-rabbit IgG horseradish peroxidase whole antibody and anti-mouse IgG horseradish peroxidase (Cell Signaling Technology, Danvers, MA, USA).

Sugiyama A., Kaisho M., Okada M., Otani K, & Yamawaki H. (2020). Decreased Expression of Canstatin in Rat Model of Monocrotaline-Induced Pulmonary Arterial Hypertension: Protective Effect of Canstatin on Right Ventricular Remodeling. International Journal of Molecular Sciences, 21(18), 6797.

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RACGAP1 Expression Detection Using Western Blotting and Immunostaining

Cited 3 times

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For western blotting and immunostaining detection of RACGAP1 expression, anti-RACGAP1 antibodies (product ID: ab2270) were used according to the manufacturer’s instructions (Abcam Plc, Cambridge, UK). An anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (product ID: SAF6698; Wako, Osaka, Japan) was used as an internal loading control for western blotting. Details of these methods have been described previously [42 (link),43 (link),44 (link),45 (link)].

Khalid M., Idichi T., Seki N., Wada M., Yamada Y., Fukuhisa H., Toda H., Kita Y., Kawasaki Y., Tanoue K., Kurahara H., Mataki Y., Maemura K, & Natsugoe S. (2019). Gene Regulation by Antitumor miR-204-5p in Pancreatic Ductal Adenocarcinoma: The Clinical Significance of Direct RACGAP1 Regulation. Cancers, 11(3), 327.

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Quantitative Analysis of Nucleolin and p53

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Soluble whole-cell lysates of the hMBs treated with 30 μM of iSN04 or AS1411 in DM for 48 h were prepared as described above. The lysates were denatured with 50 mM Tris-HCl (pH 6.8), 10% glycerol, and 2% SDS at 95°C for 5 min. Ten microgram of protein samples were subjected to SDS-PAGE on a 10% polyacrylamide gel followed by Western blotting using an iBlot 2 Dry Blotting System (Thermo Fisher Scientific). 1.0 μg/ml each of rabbit polyclonal anti-nucleolin antibody, mouse monoclonal anti-p53 antibody (PAb 240; Abcam), and mouse monoclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (5A12; Wako) were used as primary antibodies. 0.1 μg/ml each of horseradish peroxidase (HRP)-conjugated goat anti-rabbit and anti-mouse IgG antibodies (Jackson ImmunoResearch) were used as secondary antibodies, respectively. HRP activity was detected using ECL Prime reagents (GE Healthcare) and ImageQuant LAS 500. The quantities of nucleolin and p53 proteins were normalized to that of GAPDH using ImageJ software.

Shinji S., Umezawa K., Nihashi Y., Nakamura S., Shimosato T, & Takaya T. (2021). Identification of the Myogenetic Oligodeoxynucleotides (myoDNs) That Promote Differentiation of Skeletal Muscle Myoblasts by Targeting Nucleolin. Frontiers in Cell and Developmental Biology, 8, 616706.

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Antibody Characterization and Validation

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The following antibodies were purchased from the manufacturers as indicated: rabbit anti‐CD31 antibody (ab28364, dilution 1:1000; Abcam), mouse anti‐PSMA antibody (Clone 3E6, M3620, dilution 1:1000 for immunohistochemistry; Dako), donkey Alexa488‐conjugated anti‐rabbit immunoglobulin G (IgG) antibody (A21206, dilution 1:500; Life Technologies Corporation), donkey Alexa594‐conjugated anti‐mouse IgG antibody (A21203, dilution 1:500; Life Technologies Corporation), rabbit anti‐PSMA antibody (12702S, dilution 1:1000 for western blotting and immunofluorescence; Cell Signaling Technology), mouse anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) antibody (5A12, dilution 1:6000; Wako), goat Cy3‐conjugated anti‐rabbit IgG antibody (A10520, dilution 1:2000; Molecular Probes), goat Alexa488‐conjugated anti‐mouse IgG antibody (A11001, dilution 1:2000; Molecular Probes), horseradish peroxidase (HRP)‐conjugated anti‐rabbit IgG antibody (W4011, dilution 1:2000; Promega), and HRP‐conjugated anti‐mouse IgG antibody (W4021, dilution 1:2000; Promega).

Watanabe R., Maekawa M., Kiyoi T., Kurata M., Miura N., Kikugawa T., Higashiyama S, & Saika T. (2021). PSMA‐positive membranes secreted from prostate cancer cells have potency to transform vascular endothelial cells into an angiogenic state. The Prostate, 81(16), 1390-1401.

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